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Liquid chromatographic-mass spectrometric determination of endogenous gamma-hydroxybutyrate concentrations in rat brain regions and plasma.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Aug 05; 807(2):287-91.JC

Abstract

A new liquid chromatographic-mass spectrometric (LC-MS) method for determining trace concentrations of gamma-hydroxybutyric acid (GHB) in biological samples has been developed. This method utilizes solid-phase extraction for separation, deuterated GHB as an internal standard (IS) and multiple reaction monitoring (MRM) in the negative ion mode to detect the parent and product ions (103 and 57 for GHB, and 109 and 61 for D6-GHB, respectively). The assay produces excellent linearity and reproducibility, with a limit of quantification (LOQ) of about 0.1 microg/ml. The method has been applied for the determination of endogenous GHB in various rat brain regions.

Authors+Show Affiliations

Department of Pharmaceutical Sciences, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, SUNY, Buffalo, NY 14260-1200, USA. hlfung@buffalo.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15203042

Citation

Fung, Ho-Leung, et al. "Liquid Chromatographic-mass Spectrometric Determination of Endogenous Gamma-hydroxybutyrate Concentrations in Rat Brain Regions and Plasma." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 807, no. 2, 2004, pp. 287-91.
Fung HL, Haas E, Raybon J, et al. Liquid chromatographic-mass spectrometric determination of endogenous gamma-hydroxybutyrate concentrations in rat brain regions and plasma. J Chromatogr B Analyt Technol Biomed Life Sci. 2004;807(2):287-91.
Fung, H. L., Haas, E., Raybon, J., Xu, J., & Fung, S. M. (2004). Liquid chromatographic-mass spectrometric determination of endogenous gamma-hydroxybutyrate concentrations in rat brain regions and plasma. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 807(2), 287-91.
Fung HL, et al. Liquid Chromatographic-mass Spectrometric Determination of Endogenous Gamma-hydroxybutyrate Concentrations in Rat Brain Regions and Plasma. J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Aug 5;807(2):287-91. PubMed PMID: 15203042.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Liquid chromatographic-mass spectrometric determination of endogenous gamma-hydroxybutyrate concentrations in rat brain regions and plasma. AU - Fung,Ho-Leung, AU - Haas,Eric, AU - Raybon,Joseph, AU - Xu,Jian, AU - Fung,Sun-Mi, PY - 2004/01/27/received PY - 2004/04/22/revised PY - 2004/04/22/accepted PY - 2004/6/19/pubmed PY - 2005/1/19/medline PY - 2004/6/19/entrez SP - 287 EP - 91 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 807 IS - 2 N2 - A new liquid chromatographic-mass spectrometric (LC-MS) method for determining trace concentrations of gamma-hydroxybutyric acid (GHB) in biological samples has been developed. This method utilizes solid-phase extraction for separation, deuterated GHB as an internal standard (IS) and multiple reaction monitoring (MRM) in the negative ion mode to detect the parent and product ions (103 and 57 for GHB, and 109 and 61 for D6-GHB, respectively). The assay produces excellent linearity and reproducibility, with a limit of quantification (LOQ) of about 0.1 microg/ml. The method has been applied for the determination of endogenous GHB in various rat brain regions. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/15203042/Liquid_chromatographic_mass_spectrometric_determination_of_endogenous_gamma_hydroxybutyrate_concentrations_in_rat_brain_regions_and_plasma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570023204003563 DB - PRIME DP - Unbound Medicine ER -