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Gastrointestinal effects of intracerebroventricularly injected nociceptin/orphaninFQ in rats.
Peptides. 2004 Jun; 25(6):1013-20.P

Abstract

Nociceptin/orphanin FQ/(N/OFQ), a novel heptadecapeptide recently isolated from porcine and rat brain, is the endogenous ligand of the N/OFQ peptide receptor (NOP, previously known as ORL-1). In this study we examined the effects of intracerebroventricularly (icv) injected N/OFQ on gastric emptying, gastrointestinal transit, colonic propulsion and gastric acid secretion in rats. N/OFQ (0.01-10 nmol/rat) significantly delayed gastric emptying of a phenol red meal, inhibited transit of a non-absorbable charcoal marker through the small intestine and increased the mean colonic bead expulsion time. These N/OFQ-motor effects were abolished by the NOP receptor selective antagonist [NPhe(1)]N/OFQ(1-13)-NH(2) (50 nmol/rat), but were unaltered by the classical opioid receptor antagonist, naloxone (9.2 micromol/kg). Icv injected N/OFQ (10 nmol/rat) decreased gastric acid secretion in 2-h pylorus ligated rats in a naloxone sensitive manner. [NPhe(1)]N/OFQ(1-13)-NH(2) (100 nmol/rat) icv administered alone stimulated gastric acid secretion. These results indicate that N/OFQ activates via NOP receptor stimulation a central inhibitory pathway modulating gastrointestinal propulsive activity and gastric acid secretion in rats.

Authors+Show Affiliations

Department of Human Physiology and Pharmacology, University "La Sapienza" Ple A. Moro 5-00185, Rome, Italy.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15203249

Citation

Broccardo, Maria, et al. "Gastrointestinal Effects of Intracerebroventricularly Injected nociceptin/orphaninFQ in Rats." Peptides, vol. 25, no. 6, 2004, pp. 1013-20.
Broccardo M, Guerrini R, Petrella C, et al. Gastrointestinal effects of intracerebroventricularly injected nociceptin/orphaninFQ in rats. Peptides. 2004;25(6):1013-20.
Broccardo, M., Guerrini, R., Petrella, C., & Improta, G. (2004). Gastrointestinal effects of intracerebroventricularly injected nociceptin/orphaninFQ in rats. Peptides, 25(6), 1013-20.
Broccardo M, et al. Gastrointestinal Effects of Intracerebroventricularly Injected nociceptin/orphaninFQ in Rats. Peptides. 2004;25(6):1013-20. PubMed PMID: 15203249.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gastrointestinal effects of intracerebroventricularly injected nociceptin/orphaninFQ in rats. AU - Broccardo,Maria, AU - Guerrini,Remo, AU - Petrella,Carla, AU - Improta,Giovanna, PY - 2004/02/17/received PY - 2004/03/26/revised PY - 2004/03/29/accepted PY - 2004/6/19/pubmed PY - 2005/2/8/medline PY - 2004/6/19/entrez SP - 1013 EP - 20 JF - Peptides JO - Peptides VL - 25 IS - 6 N2 - Nociceptin/orphanin FQ/(N/OFQ), a novel heptadecapeptide recently isolated from porcine and rat brain, is the endogenous ligand of the N/OFQ peptide receptor (NOP, previously known as ORL-1). In this study we examined the effects of intracerebroventricularly (icv) injected N/OFQ on gastric emptying, gastrointestinal transit, colonic propulsion and gastric acid secretion in rats. N/OFQ (0.01-10 nmol/rat) significantly delayed gastric emptying of a phenol red meal, inhibited transit of a non-absorbable charcoal marker through the small intestine and increased the mean colonic bead expulsion time. These N/OFQ-motor effects were abolished by the NOP receptor selective antagonist [NPhe(1)]N/OFQ(1-13)-NH(2) (50 nmol/rat), but were unaltered by the classical opioid receptor antagonist, naloxone (9.2 micromol/kg). Icv injected N/OFQ (10 nmol/rat) decreased gastric acid secretion in 2-h pylorus ligated rats in a naloxone sensitive manner. [NPhe(1)]N/OFQ(1-13)-NH(2) (100 nmol/rat) icv administered alone stimulated gastric acid secretion. These results indicate that N/OFQ activates via NOP receptor stimulation a central inhibitory pathway modulating gastrointestinal propulsive activity and gastric acid secretion in rats. SN - 0196-9781 UR - https://www.unboundmedicine.com/medline/citation/15203249/Gastrointestinal_effects_of_intracerebroventricularly_injected_nociceptin/orphaninFQ_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0196978104001482 DB - PRIME DP - Unbound Medicine ER -