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Expression profiling and genetic alterations of the selenoproteins GI-GPx and SePP in colorectal carcinogenesis.
Nutr Cancer 2004; 48(1):6-14NC

Abstract

The trace element selenium is discussed as a chemopreventive agent in colorectal carcinogenesis. Selenocysteine-containing proteins, so-called selenoproteins, represent potential molecular targets for nutritive selenium supplementation. Due to their antioxidative potential, the selenoproteins gastrointestinal glutathione peroxidase (GI-GPx) and selenoprotein P (SePP) are considered to provide protection against reactive oxygen species (ROS), thereby reducing DNA damage and preventing development of colon cancer. GI-GPx and SePP are abundantly expressed in normal colon mucosa. Recently, we demonstrated both reduced SePP expression and increased GI-GPx expression in colorectal adenomas. In this study, we investigated the expression of SePP and GI-GPx in colorectal cancers compared with corresponding normal mucosa. Further, the occurrence of genetic alterations within the SePP and GI-GPx genes was analyzed. We observed a significant reduction or loss of SePP mRNA expression in colon cancers, whereas GI-GPx mRNA and protein expression varied between different tumor samples. In addition, we identified novel polymorphisms within the SePP and GI-GPx genes with so far unknown relevance for protein function. Our results argue against a general decrease of selenoprotein expression in colorectal carcinogenesis but imply specific differential regulation of expression of individual selenoproteins.

Authors+Show Affiliations

Medizinische Poliklinik, Universität Würzburg, Germany. al-taie.medpoli@mail.uni-wuerzburg.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15203372

Citation

Al-Taie, Oliver Hatem, et al. "Expression Profiling and Genetic Alterations of the Selenoproteins GI-GPx and SePP in Colorectal Carcinogenesis." Nutrition and Cancer, vol. 48, no. 1, 2004, pp. 6-14.
Al-Taie OH, Uceyler N, Eubner U, et al. Expression profiling and genetic alterations of the selenoproteins GI-GPx and SePP in colorectal carcinogenesis. Nutr Cancer. 2004;48(1):6-14.
Al-Taie, O. H., Uceyler, N., Eubner, U., Jakob, F., Mörk, H., Scheurlen, M., ... Köhrle, J. (2004). Expression profiling and genetic alterations of the selenoproteins GI-GPx and SePP in colorectal carcinogenesis. Nutrition and Cancer, 48(1), pp. 6-14.
Al-Taie OH, et al. Expression Profiling and Genetic Alterations of the Selenoproteins GI-GPx and SePP in Colorectal Carcinogenesis. Nutr Cancer. 2004;48(1):6-14. PubMed PMID: 15203372.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression profiling and genetic alterations of the selenoproteins GI-GPx and SePP in colorectal carcinogenesis. AU - Al-Taie,Oliver Hatem, AU - Uceyler,Nurcan, AU - Eubner,Ursula, AU - Jakob,Franz, AU - Mörk,Hubert, AU - Scheurlen,Michael, AU - Brigelius-Flohe,Regina, AU - Schöttker,Katrin, AU - Abel,Josef, AU - Thalheimer,Andreas, AU - Katzenberger,Tiemo, AU - Illert,Bertram, AU - Melcher,Ralf, AU - Köhrle,Josef, PY - 2004/6/19/pubmed PY - 2004/12/16/medline PY - 2004/6/19/entrez SP - 6 EP - 14 JF - Nutrition and cancer JO - Nutr Cancer VL - 48 IS - 1 N2 - The trace element selenium is discussed as a chemopreventive agent in colorectal carcinogenesis. Selenocysteine-containing proteins, so-called selenoproteins, represent potential molecular targets for nutritive selenium supplementation. Due to their antioxidative potential, the selenoproteins gastrointestinal glutathione peroxidase (GI-GPx) and selenoprotein P (SePP) are considered to provide protection against reactive oxygen species (ROS), thereby reducing DNA damage and preventing development of colon cancer. GI-GPx and SePP are abundantly expressed in normal colon mucosa. Recently, we demonstrated both reduced SePP expression and increased GI-GPx expression in colorectal adenomas. In this study, we investigated the expression of SePP and GI-GPx in colorectal cancers compared with corresponding normal mucosa. Further, the occurrence of genetic alterations within the SePP and GI-GPx genes was analyzed. We observed a significant reduction or loss of SePP mRNA expression in colon cancers, whereas GI-GPx mRNA and protein expression varied between different tumor samples. In addition, we identified novel polymorphisms within the SePP and GI-GPx genes with so far unknown relevance for protein function. Our results argue against a general decrease of selenoprotein expression in colorectal carcinogenesis but imply specific differential regulation of expression of individual selenoproteins. SN - 0163-5581 UR - https://www.unboundmedicine.com/medline/citation/15203372/Expression_profiling_and_genetic_alterations_of_the_selenoproteins_GI_GPx_and_SePP_in_colorectal_carcinogenesis_ L2 - http://www.tandfonline.com/doi/full/10.1207/s15327914nc4801_2 DB - PRIME DP - Unbound Medicine ER -