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Involvement of steroids in anti-inflammatory effects of PK11195 in a murine model of pleurisy.
Mediators Inflamm. 2004 Apr; 13(2):93-103.MI

Abstract

BACKGROUND

Studies on peripheral benzodiazepine receptor function have yielded a diverse list of activities of which the anti-inflammatory effects need to be further examined.

AIMS

To evaluate the role of steroids, nitric oxide and adenosine-deaminase in the anti-inflammatory effect of PK11195.

METHODS

Pleurisy was induced by intrapleural injection of carrageenan in mice pre-treated or not with PK11195. Leukocytes, exudation, adenosine-deaminase (ADA) activity and nitric oxide (NO) level were measured. Steroid involvement was evaluated by pre-treatment with D,L-aminogluthetimide before PK11195.

RESULTS

Leukocytes, exudation and NO levels were reduced by PK11195 in the early (4 h) phase. In the late (48 h) phase, PK11195 decreased leukocytes and ADA activity. D,L-aminogluthetimide reversed the effect of PK11195 on exudate (4 h), as well as total and differential leukocytes and NO levels (48 h).

CONCLUSIONS

Steroids, NO and ADA are implicated in the anti-inflammatory action of PK11195.

Authors+Show Affiliations

Department of Clinical Analysis, Center of Health Sciences, Universidade Federal de Santa Catarina, Campus Universitário, Florianópolis, SC, Brazil.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15203550

Citation

da Silva, Marcelo Barreto Spillere, et al. "Involvement of Steroids in Anti-inflammatory Effects of PK11195 in a Murine Model of Pleurisy." Mediators of Inflammation, vol. 13, no. 2, 2004, pp. 93-103.
da Silva MB, Farges RC, Fröde TS. Involvement of steroids in anti-inflammatory effects of PK11195 in a murine model of pleurisy. Mediators Inflamm. 2004;13(2):93-103.
da Silva, M. B., Farges, R. C., & Fröde, T. S. (2004). Involvement of steroids in anti-inflammatory effects of PK11195 in a murine model of pleurisy. Mediators of Inflammation, 13(2), 93-103.
da Silva MB, Farges RC, Fröde TS. Involvement of Steroids in Anti-inflammatory Effects of PK11195 in a Murine Model of Pleurisy. Mediators Inflamm. 2004;13(2):93-103. PubMed PMID: 15203550.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of steroids in anti-inflammatory effects of PK11195 in a murine model of pleurisy. AU - da Silva,Marcelo Barreto Spillere, AU - Farges,Roseli Coimbra, AU - Fröde,Tânia Silvia, PY - 2004/6/19/pubmed PY - 2005/3/30/medline PY - 2004/6/19/entrez SP - 93 EP - 103 JF - Mediators of inflammation JO - Mediators Inflamm VL - 13 IS - 2 N2 - BACKGROUND: Studies on peripheral benzodiazepine receptor function have yielded a diverse list of activities of which the anti-inflammatory effects need to be further examined. AIMS: To evaluate the role of steroids, nitric oxide and adenosine-deaminase in the anti-inflammatory effect of PK11195. METHODS: Pleurisy was induced by intrapleural injection of carrageenan in mice pre-treated or not with PK11195. Leukocytes, exudation, adenosine-deaminase (ADA) activity and nitric oxide (NO) level were measured. Steroid involvement was evaluated by pre-treatment with D,L-aminogluthetimide before PK11195. RESULTS: Leukocytes, exudation and NO levels were reduced by PK11195 in the early (4 h) phase. In the late (48 h) phase, PK11195 decreased leukocytes and ADA activity. D,L-aminogluthetimide reversed the effect of PK11195 on exudate (4 h), as well as total and differential leukocytes and NO levels (48 h). CONCLUSIONS: Steroids, NO and ADA are implicated in the anti-inflammatory action of PK11195. SN - 0962-9351 UR - https://www.unboundmedicine.com/medline/citation/15203550/Involvement_of_steroids_in_anti_inflammatory_effects_of_PK11195_in_a_murine_model_of_pleurisy_ L2 - https://doi.org/10.1080/09629350410001688486 DB - PRIME DP - Unbound Medicine ER -