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[The expression and activity detection of a variant N protein of SARS-CoV].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004 Jul; 20(4):422-4.XB

Abstract

AIM

To construct an expression vector pGEX-2T/N, and to express the fusion protein consisting of N protein of SARS-CoV and GST in E.coli.

METHODS

The N region gene of SARS-CoV was cloned by RT-PCR. The expression vector was constructed by DNA recombination. The recombinant plasmid was transformed into E.coli BL21(DE3). The expression of the fusion protein was detected by Western blot.

RESULTS

(1) As compared with the sequences in GenBank, 20 bp were deleted in DNA sequence of the cloned N protein. (2) The fusion protein GST-N was soluble. Western blot analysis showed that the reaction of GST-N to anti-SARS-CoV sera was positive.

CONCLUSION

The pGEX-2T/N has been constructed and expressed in the form of fusion protein GST-N successfully, which lays the foundation for further study of SARS-CoV N protein.

Authors+Show Affiliations

Department of Immunology, Institute of Infectious Diseases, 302th Hospital of PLA, Beijing 100039, China. qiy2004@sohu.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15207085

Citation

Qi, Yang, et al. "[The Expression and Activity Detection of a Variant N Protein of SARS-CoV]." Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, vol. 20, no. 4, 2004, pp. 422-4.
Qi Y, Zheng Y, Shu CL, et al. [The expression and activity detection of a variant N protein of SARS-CoV]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004;20(4):422-4.
Qi, Y., Zheng, Y., Shu, C. L., Jiang, L., Hu, Y., Mao, P. Y., & Cheng, Y. (2004). [The expression and activity detection of a variant N protein of SARS-CoV]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, 20(4), 422-4.
Qi Y, et al. [The Expression and Activity Detection of a Variant N Protein of SARS-CoV]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004;20(4):422-4. PubMed PMID: 15207085.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [The expression and activity detection of a variant N protein of SARS-CoV]. AU - Qi,Yang, AU - Zheng,Yu, AU - Shu,Cui-li, AU - Jiang,Li, AU - Hu,Yan, AU - Mao,Pan-yong, AU - Cheng,Yun, PY - 2004/6/23/pubmed PY - 2005/2/8/medline PY - 2004/6/23/entrez SP - 422 EP - 4 JF - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology JO - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi VL - 20 IS - 4 N2 - AIM: To construct an expression vector pGEX-2T/N, and to express the fusion protein consisting of N protein of SARS-CoV and GST in E.coli. METHODS: The N region gene of SARS-CoV was cloned by RT-PCR. The expression vector was constructed by DNA recombination. The recombinant plasmid was transformed into E.coli BL21(DE3). The expression of the fusion protein was detected by Western blot. RESULTS: (1) As compared with the sequences in GenBank, 20 bp were deleted in DNA sequence of the cloned N protein. (2) The fusion protein GST-N was soluble. Western blot analysis showed that the reaction of GST-N to anti-SARS-CoV sera was positive. CONCLUSION: The pGEX-2T/N has been constructed and expressed in the form of fusion protein GST-N successfully, which lays the foundation for further study of SARS-CoV N protein. SN - 1007-8738 UR - https://www.unboundmedicine.com/medline/citation/15207085/[The_expression_and_activity_detection_of_a_variant_N_protein_of_SARS_CoV]_ L2 - https://www.diseaseinfosearch.org/result/6415 DB - PRIME DP - Unbound Medicine ER -