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Association of potassium channel Kv3.4 subunits with pre- and post-synaptic structures in brainstem and spinal cord.
Neuroscience. 2004; 126(4):1001-10.N

Abstract

Voltage-gated K+ channels (Kv) are divided into eight subfamilies (Kv1-8) and play a major role in determining the excitability of neurones. Members of the Kv3 subfamily are highly abundant in the CNS, with each Kv3 gene (Kv3.1-Kv3.4) exhibiting a unique pattern of expression, although single neurones can express more than one subtype. Of the Kv3 subunits relatively little is known of the Kv3.4 subunit distribution in the nervous system, particularly in the brainstem and spinal cord of the rat. We performed immunohistochemistry to determine both the cellular and sub-cellular distribution of the Kv3.4 subunit in these areas. Kv3.4 subunit immunoreactivity (Kv3.4-IR) was widespread, with dense, punctate staining in many regions including the intermediolateral cell column (IML) and the dorsal vagal nucleus (DVN), nucleus ambiguus (NA) and nucleus tractus solitarius (NTS). In the ventral horn a presynaptic location was confirmed by co-localization of Kv3.4-IR with the synaptic vesicle protein, SV2 and also with the glutamate vesicle markers vesicular glutamate transporter (VGluT) 1, VGluT2 or the glycine transporter GlyT2, suggesting a role for the channel in both excitatory and inhibitory neurotransmission. Electron microscopy confirmed a presynaptic terminal location of Kv3.4-IR in the VH, IML, DVN, NA and NTS. Interestingly however, patches of Kv3.4-IR were also revealed postsynaptically in dendritic and somatic structures throughout these areas. This staining was striking due to its localization at synaptic junctions at terminals with morphological features consistent with excitatory functions, suggesting an association with the postsynaptic density. Therefore the pre and postsynaptic localization of Kv3.4-IR suggests a role both in the control of transmitter release and in regulating neuronal excitability.

Authors+Show Affiliations

School of Biomedical Sciences, University of Leeds, Leeds LS2 9NQ, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15207333

Citation

Brooke, R E., et al. "Association of Potassium Channel Kv3.4 Subunits With Pre- and Post-synaptic Structures in Brainstem and Spinal Cord." Neuroscience, vol. 126, no. 4, 2004, pp. 1001-10.
Brooke RE, Atkinson L, Batten TF, et al. Association of potassium channel Kv3.4 subunits with pre- and post-synaptic structures in brainstem and spinal cord. Neuroscience. 2004;126(4):1001-10.
Brooke, R. E., Atkinson, L., Batten, T. F., Deuchars, S. A., & Deuchars, J. (2004). Association of potassium channel Kv3.4 subunits with pre- and post-synaptic structures in brainstem and spinal cord. Neuroscience, 126(4), 1001-10.
Brooke RE, et al. Association of Potassium Channel Kv3.4 Subunits With Pre- and Post-synaptic Structures in Brainstem and Spinal Cord. Neuroscience. 2004;126(4):1001-10. PubMed PMID: 15207333.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association of potassium channel Kv3.4 subunits with pre- and post-synaptic structures in brainstem and spinal cord. AU - Brooke,R E, AU - Atkinson,L, AU - Batten,T F C, AU - Deuchars,S A, AU - Deuchars,J, PY - 2004/03/29/accepted PY - 2004/6/23/pubmed PY - 2004/10/9/medline PY - 2004/6/23/entrez SP - 1001 EP - 10 JF - Neuroscience JO - Neuroscience VL - 126 IS - 4 N2 - Voltage-gated K+ channels (Kv) are divided into eight subfamilies (Kv1-8) and play a major role in determining the excitability of neurones. Members of the Kv3 subfamily are highly abundant in the CNS, with each Kv3 gene (Kv3.1-Kv3.4) exhibiting a unique pattern of expression, although single neurones can express more than one subtype. Of the Kv3 subunits relatively little is known of the Kv3.4 subunit distribution in the nervous system, particularly in the brainstem and spinal cord of the rat. We performed immunohistochemistry to determine both the cellular and sub-cellular distribution of the Kv3.4 subunit in these areas. Kv3.4 subunit immunoreactivity (Kv3.4-IR) was widespread, with dense, punctate staining in many regions including the intermediolateral cell column (IML) and the dorsal vagal nucleus (DVN), nucleus ambiguus (NA) and nucleus tractus solitarius (NTS). In the ventral horn a presynaptic location was confirmed by co-localization of Kv3.4-IR with the synaptic vesicle protein, SV2 and also with the glutamate vesicle markers vesicular glutamate transporter (VGluT) 1, VGluT2 or the glycine transporter GlyT2, suggesting a role for the channel in both excitatory and inhibitory neurotransmission. Electron microscopy confirmed a presynaptic terminal location of Kv3.4-IR in the VH, IML, DVN, NA and NTS. Interestingly however, patches of Kv3.4-IR were also revealed postsynaptically in dendritic and somatic structures throughout these areas. This staining was striking due to its localization at synaptic junctions at terminals with morphological features consistent with excitatory functions, suggesting an association with the postsynaptic density. Therefore the pre and postsynaptic localization of Kv3.4-IR suggests a role both in the control of transmitter release and in regulating neuronal excitability. SN - 0306-4522 UR - https://www.unboundmedicine.com/medline/citation/15207333/Association_of_potassium_channel_Kv3_4_subunits_with_pre__and_post_synaptic_structures_in_brainstem_and_spinal_cord_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S030645220400243X DB - PRIME DP - Unbound Medicine ER -