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Fermentation performance of engineered and evolved xylose-fermenting Saccharomyces cerevisiae strains.
Biotechnol Bioeng. 2004 Jul 05; 87(1):90-8.BB

Abstract

Lignocellulose hydrolysate is an abundant substrate for bioethanol production. The ideal microorganism for such a fermentation process should combine rapid and efficient conversion of the available carbon sources to ethanol with high tolerance to ethanol and to inhibitory components in the hydrolysate. A particular biological problem are the pentoses, which are not naturally metabolized by the main industrial ethanol producer Saccharomyces cerevisiae. Several recombinant, mutated, and evolved xylose fermenting S. cerevisiae strains have been developed recently. We compare here the fermentation performance and robustness of eight recombinant strains and two evolved populations on glucose/xylose mixtures in defined and lignocellulose hydrolysate-containing medium. Generally, the polyploid industrial strains depleted xylose faster and were more resistant to the hydrolysate than the laboratory strains. The industrial strains accumulated, however, up to 30% more xylitol and therefore produced less ethanol than the haploid strains. The three most attractive strains were the mutated and selected, extremely rapid xylose consumer TMB3400, the evolved C5 strain with the highest achieved ethanol titer, and the engineered industrial F12 strain with by far the highest robustness to the lignocellulosic hydrolysate.

Authors+Show Affiliations

Institute of Biotechnology, ETH Zürich, CH-8093 Zürich, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15211492

Citation

Sonderegger, Marco, et al. "Fermentation Performance of Engineered and Evolved Xylose-fermenting Saccharomyces Cerevisiae Strains." Biotechnology and Bioengineering, vol. 87, no. 1, 2004, pp. 90-8.
Sonderegger M, Jeppsson M, Larsson C, et al. Fermentation performance of engineered and evolved xylose-fermenting Saccharomyces cerevisiae strains. Biotechnol Bioeng. 2004;87(1):90-8.
Sonderegger, M., Jeppsson, M., Larsson, C., Gorwa-Grauslund, M. F., Boles, E., Olsson, L., Spencer-Martins, I., Hahn-Hägerdal, B., & Sauer, U. (2004). Fermentation performance of engineered and evolved xylose-fermenting Saccharomyces cerevisiae strains. Biotechnology and Bioengineering, 87(1), 90-8.
Sonderegger M, et al. Fermentation Performance of Engineered and Evolved Xylose-fermenting Saccharomyces Cerevisiae Strains. Biotechnol Bioeng. 2004 Jul 5;87(1):90-8. PubMed PMID: 15211492.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fermentation performance of engineered and evolved xylose-fermenting Saccharomyces cerevisiae strains. AU - Sonderegger,Marco, AU - Jeppsson,Marie, AU - Larsson,Christer, AU - Gorwa-Grauslund,Marie-Françoise, AU - Boles,Eckhard, AU - Olsson,Lisbeth, AU - Spencer-Martins,Isabel, AU - Hahn-Hägerdal,Bärbel, AU - Sauer,Uwe, PY - 2004/6/24/pubmed PY - 2005/1/14/medline PY - 2004/6/24/entrez SP - 90 EP - 8 JF - Biotechnology and bioengineering JO - Biotechnol Bioeng VL - 87 IS - 1 N2 - Lignocellulose hydrolysate is an abundant substrate for bioethanol production. The ideal microorganism for such a fermentation process should combine rapid and efficient conversion of the available carbon sources to ethanol with high tolerance to ethanol and to inhibitory components in the hydrolysate. A particular biological problem are the pentoses, which are not naturally metabolized by the main industrial ethanol producer Saccharomyces cerevisiae. Several recombinant, mutated, and evolved xylose fermenting S. cerevisiae strains have been developed recently. We compare here the fermentation performance and robustness of eight recombinant strains and two evolved populations on glucose/xylose mixtures in defined and lignocellulose hydrolysate-containing medium. Generally, the polyploid industrial strains depleted xylose faster and were more resistant to the hydrolysate than the laboratory strains. The industrial strains accumulated, however, up to 30% more xylitol and therefore produced less ethanol than the haploid strains. The three most attractive strains were the mutated and selected, extremely rapid xylose consumer TMB3400, the evolved C5 strain with the highest achieved ethanol titer, and the engineered industrial F12 strain with by far the highest robustness to the lignocellulosic hydrolysate. SN - 0006-3592 UR - https://www.unboundmedicine.com/medline/citation/15211492/Fermentation_performance_of_engineered_and_evolved_xylose_fermenting_Saccharomyces_cerevisiae_strains_ DB - PRIME DP - Unbound Medicine ER -