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Transdermal delivery of insulin using microneedles in vivo.
Pharm Res. 2004 Jun; 21(6):947-52.PR

Abstract

PURPOSE

The purpose of this study was to design and fabricate arrays of solid microneedles and insert them into the skin of diabetic hairless rats for transdermal delivery of insulin to lower blood glucose level.

METHODS

Arrays containing 105 microneedles were laser-cut from stainless steel metal sheets and inserted into the skin of anesthetized hairless rats with streptozotocin-induced diabetes. During and after microneedle treatment, an insulin solution (100 or 500 U/ml) was placed in contact with the skin for 4 h. Microneedles were removed 10 s, 10 min, or 4 h after initiating transdermal insulin delivery. Blood glucose levels were measured electrochemically every 30 min. Plasma insulin concentration was determined by radioimmunoassay at the end of most experiments.

RESULTS

Arrays of microneedles were fabricated and demonstrated to insert fully into hairless rat skin in vivo. Microneedles increased skin permeability to insulin, which rapidly and steadily reduced blood glucose levels to an extent similar to 0.05-0.5 U insulin injected subcutaneously. Plasma insulin concentrations were directly measured to be 0.5-7.4 ng/ml. Higher donor solution insulin concentration, shorter insertion time, and fewer repeated insertions resulted in larger drops in blood glucose level and larger plasma insulin concentrations.

CONCLUSIONS

Solid metal microneedles are capable of increasing transdermal insulin delivery and lowering blood glucose levels by as much as 80% in diabetic hairless rats in vivo.

Authors+Show Affiliations

School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15212158

Citation

Martanto, Wijaya, et al. "Transdermal Delivery of Insulin Using Microneedles in Vivo." Pharmaceutical Research, vol. 21, no. 6, 2004, pp. 947-52.
Martanto W, Davis SP, Holiday NR, et al. Transdermal delivery of insulin using microneedles in vivo. Pharm Res. 2004;21(6):947-52.
Martanto, W., Davis, S. P., Holiday, N. R., Wang, J., Gill, H. S., & Prausnitz, M. R. (2004). Transdermal delivery of insulin using microneedles in vivo. Pharmaceutical Research, 21(6), 947-52.
Martanto W, et al. Transdermal Delivery of Insulin Using Microneedles in Vivo. Pharm Res. 2004;21(6):947-52. PubMed PMID: 15212158.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transdermal delivery of insulin using microneedles in vivo. AU - Martanto,Wijaya, AU - Davis,Shawn P, AU - Holiday,Nicholas R, AU - Wang,Jenny, AU - Gill,Harvinder S, AU - Prausnitz,Mark R, PY - 2004/6/24/pubmed PY - 2005/6/10/medline PY - 2004/6/24/entrez SP - 947 EP - 52 JF - Pharmaceutical research JO - Pharm Res VL - 21 IS - 6 N2 - PURPOSE: The purpose of this study was to design and fabricate arrays of solid microneedles and insert them into the skin of diabetic hairless rats for transdermal delivery of insulin to lower blood glucose level. METHODS: Arrays containing 105 microneedles were laser-cut from stainless steel metal sheets and inserted into the skin of anesthetized hairless rats with streptozotocin-induced diabetes. During and after microneedle treatment, an insulin solution (100 or 500 U/ml) was placed in contact with the skin for 4 h. Microneedles were removed 10 s, 10 min, or 4 h after initiating transdermal insulin delivery. Blood glucose levels were measured electrochemically every 30 min. Plasma insulin concentration was determined by radioimmunoassay at the end of most experiments. RESULTS: Arrays of microneedles were fabricated and demonstrated to insert fully into hairless rat skin in vivo. Microneedles increased skin permeability to insulin, which rapidly and steadily reduced blood glucose levels to an extent similar to 0.05-0.5 U insulin injected subcutaneously. Plasma insulin concentrations were directly measured to be 0.5-7.4 ng/ml. Higher donor solution insulin concentration, shorter insertion time, and fewer repeated insertions resulted in larger drops in blood glucose level and larger plasma insulin concentrations. CONCLUSIONS: Solid metal microneedles are capable of increasing transdermal insulin delivery and lowering blood glucose levels by as much as 80% in diabetic hairless rats in vivo. SN - 0724-8741 UR - https://www.unboundmedicine.com/medline/citation/15212158/Transdermal_delivery_of_insulin_using_microneedles_in_vivo_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=15212158.ui DB - PRIME DP - Unbound Medicine ER -