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Canine distemper virus infection of canine footpad epidermis.
Vet Dermatol. 2004 Jun; 15(3):159-67.VD

Abstract

Infection of the footpad epidermis can occur in natural canine distemper virus (CDV) infection of dogs. Footpads from 19 dogs experimentally inoculated with virulent distemper strain A75/17 and from two nonexposed dogs were examined histopathologically and assessed for the presence of viral antigen and nucleoprotein mRNA, as well as number of inflammatory and apoptotic cells. Dogs were divided into four groups based on inoculation status and postmortem examination: inoculated dogs with severe distemper (group 1, n = 7); inoculated dogs with mild distemper (group 2, n = 4); inoculated dogs without distemper (group 3, n = 8); and noninoculated dogs (group 4, n = 2). Footpads from dogs of all groups had a comparably thick epidermis. Eosinophilic viral inclusions and syncytial cells were present in footpad epidermis of one dog of group 1. Footpads of group 1 dogs contained viral antigen and mRNA in the epidermis with strongest staining in a subcorneal location. Additionally, in these dogs footpad dermal structures including eccrine glands and vascular walls were positive for virus particles. No CDV antigen or mRNA was present in the footpad epidermis and dermis of any other dog. Group 1 dogs had more CD3-positive cells and apoptotic cells within the basal layer of the epidermis when compared to the other groups. These findings demonstrate that in experimental infection CDV antigen and mRNA were colocalized in all layers of the infected canine footpad epidermis. The scarcity of overt pathological reactions with absence of keratinocyte degeneration indicates a noncytocidal persisting infection of footpad keratinocytes by CDV.

Authors+Show Affiliations

Institut für Tierpathologie, Abteilung Klinische Forschung Universität Bern, Berne, Switzerland. agroene@tiho-hannover.deNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15214952

Citation

Gröne, Andrea, et al. "Canine Distemper Virus Infection of Canine Footpad Epidermis." Veterinary Dermatology, vol. 15, no. 3, 2004, pp. 159-67.
Gröne A, Doherr MG, Zurbriggen A. Canine distemper virus infection of canine footpad epidermis. Vet Dermatol. 2004;15(3):159-67.
Gröne, A., Doherr, M. G., & Zurbriggen, A. (2004). Canine distemper virus infection of canine footpad epidermis. Veterinary Dermatology, 15(3), 159-67.
Gröne A, Doherr MG, Zurbriggen A. Canine Distemper Virus Infection of Canine Footpad Epidermis. Vet Dermatol. 2004;15(3):159-67. PubMed PMID: 15214952.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Canine distemper virus infection of canine footpad epidermis. AU - Gröne,Andrea, AU - Doherr,Marcus G, AU - Zurbriggen,Andreas, PY - 2004/6/25/pubmed PY - 2004/8/13/medline PY - 2004/6/25/entrez SP - 159 EP - 67 JF - Veterinary dermatology JO - Vet. Dermatol. VL - 15 IS - 3 N2 - Infection of the footpad epidermis can occur in natural canine distemper virus (CDV) infection of dogs. Footpads from 19 dogs experimentally inoculated with virulent distemper strain A75/17 and from two nonexposed dogs were examined histopathologically and assessed for the presence of viral antigen and nucleoprotein mRNA, as well as number of inflammatory and apoptotic cells. Dogs were divided into four groups based on inoculation status and postmortem examination: inoculated dogs with severe distemper (group 1, n = 7); inoculated dogs with mild distemper (group 2, n = 4); inoculated dogs without distemper (group 3, n = 8); and noninoculated dogs (group 4, n = 2). Footpads from dogs of all groups had a comparably thick epidermis. Eosinophilic viral inclusions and syncytial cells were present in footpad epidermis of one dog of group 1. Footpads of group 1 dogs contained viral antigen and mRNA in the epidermis with strongest staining in a subcorneal location. Additionally, in these dogs footpad dermal structures including eccrine glands and vascular walls were positive for virus particles. No CDV antigen or mRNA was present in the footpad epidermis and dermis of any other dog. Group 1 dogs had more CD3-positive cells and apoptotic cells within the basal layer of the epidermis when compared to the other groups. These findings demonstrate that in experimental infection CDV antigen and mRNA were colocalized in all layers of the infected canine footpad epidermis. The scarcity of overt pathological reactions with absence of keratinocyte degeneration indicates a noncytocidal persisting infection of footpad keratinocytes by CDV. SN - 0959-4493 UR - https://www.unboundmedicine.com/medline/citation/15214952/Canine_distemper_virus_infection_of_canine_footpad_epidermis_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0959-4493&date=2004&volume=15&issue=3&spage=159 DB - PRIME DP - Unbound Medicine ER -