Tags

Type your tag names separated by a space and hit enter

Selection of resistance of telithromycin against Haemophilus influenzae, Moraxella catarrhalis and streptococci in comparison with macrolides.
J Antimicrob Chemother. 2004 Aug; 54(2):542-5.JA

Abstract

OBJECTIVE

The in vitro abilities of telithromycin, azithromycin and clarithromycin to select for resistance were compared by testing isolates of Haemophilus influenzae, Moraxella catarrhalis, Streptococcus pneumoniae and beta-haemolytic streptococci.

METHODS

Five strains each of beta-lactamase-positive and beta-lactamase-negative H. influenzae, beta-lactamase-positive and beta-lactamase-negative M. catarrhalis, S. pneumoniae, beta-haemolytic group A, group C and group G streptococci and three strains of beta-lactamase-negative ampicillin-resistant H. influenzae were evaluated. Development of resistance was determined by multi-step and single-step methodologies. For multi-step studies, MIC values were determined after five serial passages on antibiotic-gradient plates and after 10 serial passages on antibiotic-free plates. Acquisition of resistance was defined as an increase of >/=4-fold from the starting MIC. In single-step studies, the rate of spontaneous mutations was calculated after a passage on plates containing antibiotics at concentrations equal to the highest NCCLS breakpoints.

RESULTS

Azithromycin, clarithromycin and telithromycin gave a >/=4-fold increase in 20, 20 and 10 streptococcus strains, in 4, 5 and 0 H. influenzae strains and in 2, 7 and 4 M. catarrhalis strains, respectively. After 10 passages on antibiotic-free plates, 21/26 strains for azithromycin, 22/32 for clarithromycin and 1/14 for telithromycin maintained high MIC values. In single-step studies, the frequency of mutations was <10(-10) for H. influenzae and M. catarrhalis for telithromycin, azithromycin and clarithromycin. Telithromycin induced mutations at a lower rate than azithromycin and clarithromycin in streptococcal strains.

CONCLUSION

Telithromycin showed a very limited ability to select for resistance in respiratory pathogens compared with azithromycin and clarithromycin.

Authors+Show Affiliations

Laboratory of Clinical Microbiology, Department of Clinical Sciences L. Sacco Teaching Hospital, University of Milan, Via GB Grassi 74, 20157 Milan, Italy. microbio@unimi.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15215227

Citation

Drago, Lorenzo, et al. "Selection of Resistance of Telithromycin Against Haemophilus Influenzae, Moraxella Catarrhalis and Streptococci in Comparison With Macrolides." The Journal of Antimicrobial Chemotherapy, vol. 54, no. 2, 2004, pp. 542-5.
Drago L, De Vecchi E, Nicola L, et al. Selection of resistance of telithromycin against Haemophilus influenzae, Moraxella catarrhalis and streptococci in comparison with macrolides. J Antimicrob Chemother. 2004;54(2):542-5.
Drago, L., De Vecchi, E., Nicola, L., Colombo, A., & Gismondo, M. R. (2004). Selection of resistance of telithromycin against Haemophilus influenzae, Moraxella catarrhalis and streptococci in comparison with macrolides. The Journal of Antimicrobial Chemotherapy, 54(2), 542-5.
Drago L, et al. Selection of Resistance of Telithromycin Against Haemophilus Influenzae, Moraxella Catarrhalis and Streptococci in Comparison With Macrolides. J Antimicrob Chemother. 2004;54(2):542-5. PubMed PMID: 15215227.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selection of resistance of telithromycin against Haemophilus influenzae, Moraxella catarrhalis and streptococci in comparison with macrolides. AU - Drago,Lorenzo, AU - De Vecchi,Elena, AU - Nicola,Lucia, AU - Colombo,Alberto, AU - Gismondo,Maria Rita, Y1 - 2004/06/23/ PY - 2004/6/25/pubmed PY - 2004/10/16/medline PY - 2004/6/25/entrez SP - 542 EP - 5 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 54 IS - 2 N2 - OBJECTIVE: The in vitro abilities of telithromycin, azithromycin and clarithromycin to select for resistance were compared by testing isolates of Haemophilus influenzae, Moraxella catarrhalis, Streptococcus pneumoniae and beta-haemolytic streptococci. METHODS: Five strains each of beta-lactamase-positive and beta-lactamase-negative H. influenzae, beta-lactamase-positive and beta-lactamase-negative M. catarrhalis, S. pneumoniae, beta-haemolytic group A, group C and group G streptococci and three strains of beta-lactamase-negative ampicillin-resistant H. influenzae were evaluated. Development of resistance was determined by multi-step and single-step methodologies. For multi-step studies, MIC values were determined after five serial passages on antibiotic-gradient plates and after 10 serial passages on antibiotic-free plates. Acquisition of resistance was defined as an increase of >/=4-fold from the starting MIC. In single-step studies, the rate of spontaneous mutations was calculated after a passage on plates containing antibiotics at concentrations equal to the highest NCCLS breakpoints. RESULTS: Azithromycin, clarithromycin and telithromycin gave a >/=4-fold increase in 20, 20 and 10 streptococcus strains, in 4, 5 and 0 H. influenzae strains and in 2, 7 and 4 M. catarrhalis strains, respectively. After 10 passages on antibiotic-free plates, 21/26 strains for azithromycin, 22/32 for clarithromycin and 1/14 for telithromycin maintained high MIC values. In single-step studies, the frequency of mutations was <10(-10) for H. influenzae and M. catarrhalis for telithromycin, azithromycin and clarithromycin. Telithromycin induced mutations at a lower rate than azithromycin and clarithromycin in streptococcal strains. CONCLUSION: Telithromycin showed a very limited ability to select for resistance in respiratory pathogens compared with azithromycin and clarithromycin. SN - 0305-7453 UR - https://www.unboundmedicine.com/medline/citation/15215227/Selection_of_resistance_of_telithromycin_against_Haemophilus_influenzae_Moraxella_catarrhalis_and_streptococci_in_comparison_with_macrolides_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkh339 DB - PRIME DP - Unbound Medicine ER -