Tags

Type your tag names separated by a space and hit enter

Metabolomic differentiation of Cannabis sativa cultivars using 1H NMR spectroscopy and principal component analysis.
J Nat Prod. 2004 Jun; 67(6):953-7.JN

Abstract

The metabolomic analysis of 12 Cannabis sativa cultivars was carried out by 1H NMR spectroscopy and multivariate analysis techniques. Principal component analysis (PCA) of the 1H NMR spectra showed a clear discrimination between those samples by principal component 1 (PC1) and principal component 3 (PC3) in cannabinoid fraction. The loading plot of PC value obtained from all 1)H NMR signals shows that Delta9-tetrahydrocannabinolic acid (THCA) and cannabidiolic acid (CBDA) are important metabolites to differentiate the cultivars from each other. The discrimination of the cultivars could also be obtained from a water extract containing carbohydrates and amino acids. The level of sucrose, glucose, asparagine, and glutamic acid are found to be major discriminating metabolites of these cultivars. This method allows an efficient differentiation between cannabis cultivars without any prepurification steps.

Authors+Show Affiliations

Division of Pharmacognosy, Section Metabolomics, Institute of Biology, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15217272

Citation

Choi, Young Hae, et al. "Metabolomic Differentiation of Cannabis Sativa Cultivars Using 1H NMR Spectroscopy and Principal Component Analysis." Journal of Natural Products, vol. 67, no. 6, 2004, pp. 953-7.
Choi YH, Kim HK, Hazekamp A, et al. Metabolomic differentiation of Cannabis sativa cultivars using 1H NMR spectroscopy and principal component analysis. J Nat Prod. 2004;67(6):953-7.
Choi, Y. H., Kim, H. K., Hazekamp, A., Erkelens, C., Lefeber, A. W., & Verpoorte, R. (2004). Metabolomic differentiation of Cannabis sativa cultivars using 1H NMR spectroscopy and principal component analysis. Journal of Natural Products, 67(6), 953-7.
Choi YH, et al. Metabolomic Differentiation of Cannabis Sativa Cultivars Using 1H NMR Spectroscopy and Principal Component Analysis. J Nat Prod. 2004;67(6):953-7. PubMed PMID: 15217272.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolomic differentiation of Cannabis sativa cultivars using 1H NMR spectroscopy and principal component analysis. AU - Choi,Young Hae, AU - Kim,Hye Kyong, AU - Hazekamp,Arno, AU - Erkelens,Cornelis, AU - Lefeber,Alfons W M, AU - Verpoorte,Robert, PY - 2004/6/26/pubmed PY - 2004/8/10/medline PY - 2004/6/26/entrez SP - 953 EP - 7 JF - Journal of natural products JO - J Nat Prod VL - 67 IS - 6 N2 - The metabolomic analysis of 12 Cannabis sativa cultivars was carried out by 1H NMR spectroscopy and multivariate analysis techniques. Principal component analysis (PCA) of the 1H NMR spectra showed a clear discrimination between those samples by principal component 1 (PC1) and principal component 3 (PC3) in cannabinoid fraction. The loading plot of PC value obtained from all 1)H NMR signals shows that Delta9-tetrahydrocannabinolic acid (THCA) and cannabidiolic acid (CBDA) are important metabolites to differentiate the cultivars from each other. The discrimination of the cultivars could also be obtained from a water extract containing carbohydrates and amino acids. The level of sucrose, glucose, asparagine, and glutamic acid are found to be major discriminating metabolites of these cultivars. This method allows an efficient differentiation between cannabis cultivars without any prepurification steps. SN - 0163-3864 UR - https://www.unboundmedicine.com/medline/citation/15217272/Metabolomic_differentiation_of_Cannabis_sativa_cultivars_using_1H_NMR_spectroscopy_and_principal_component_analysis_ L2 - https://doi.org/10.1021/np049919c DB - PRIME DP - Unbound Medicine ER -