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N-cadherin expression and epithelial-mesenchymal transition in pancreatic carcinoma.
Clin Cancer Res. 2004 Jun 15; 10(12 Pt 1):4125-33.CC

Abstract

PURPOSE

Loss of intercellular adhesion and increased cell motility promote tumor cell invasion. In the present study, E- and N-cadherin, members of the classical cadherin family, are investigated as inducers of epithelial-to-mesenchymal transition (EMT) that is thought to play a fundamental role during the early steps of invasion and metastasis of carcinomas. Cell growth factors are known to regulate cell adhesion molecules. The purpose of the study presented here was to investigate whether a gain in N-cadherin in pancreatic cancer is involved in the process of metastasis via EMT and whether its expression is affected by growth factors.

EXPERIMENTAL DESIGN

We immunohistochemically examined the expression of N- and E-cadherins and vimentin, a mesenchymal marker, in pancreatic primary and metastatic tumors. Correlations among the expressions of N-cadherin, transforming growth factor (TGF)beta, and fibroblast growth factor 2 was evaluated in both tumors, and the induction of cadherin and vimentin by growth factors was examined in cultured cell lines.

RESULTS

N-cadherin expression was observed in 13 of 30 primary tumors and in 8 of 15 metastatic tumors. N-cadherin expression correlated with neural invasion (P = 0.008), histological type (P = 0.043), fibroblast growth factor expression in primary tumors (P = 0.007), and TGF expression (P = 0.004) and vimentin (P = 0.01) in metastatic tumors. Vimentin, a mesenchymal marker, was observed in a few cancer cells of primary tumor but was substantially expressed in liver metastasis. TGF stimulated N-cadherin and vimentin protein expression and decreased E-cadherin expression of Panc-1 cells with morphological change.

CONCLUSION

This study provided the morphological evidence of EMT in pancreatic carcinoma and revealed that overexpression of N-cadherin is involved in EMT and is affected by growth factors.

Authors+Show Affiliations

Department of Surgery and Surgical Basic Science, Graduate School of Medicine, Kyoto University, Kyoto, Japan. sana@kuhp.kyoto-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15217949

Citation

Nakajima, Sanae, et al. "N-cadherin Expression and Epithelial-mesenchymal Transition in Pancreatic Carcinoma." Clinical Cancer Research : an Official Journal of the American Association for Cancer Research, vol. 10, no. 12 Pt 1, 2004, pp. 4125-33.
Nakajima S, Doi R, Toyoda E, et al. N-cadherin expression and epithelial-mesenchymal transition in pancreatic carcinoma. Clin Cancer Res. 2004;10(12 Pt 1):4125-33.
Nakajima, S., Doi, R., Toyoda, E., Tsuji, S., Wada, M., Koizumi, M., Tulachan, S. S., Ito, D., Kami, K., Mori, T., Kawaguchi, Y., Fujimoto, K., Hosotani, R., & Imamura, M. (2004). N-cadherin expression and epithelial-mesenchymal transition in pancreatic carcinoma. Clinical Cancer Research : an Official Journal of the American Association for Cancer Research, 10(12 Pt 1), 4125-33.
Nakajima S, et al. N-cadherin Expression and Epithelial-mesenchymal Transition in Pancreatic Carcinoma. Clin Cancer Res. 2004 Jun 15;10(12 Pt 1):4125-33. PubMed PMID: 15217949.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - N-cadherin expression and epithelial-mesenchymal transition in pancreatic carcinoma. AU - Nakajima,Sanae, AU - Doi,Ryuichiro, AU - Toyoda,Eiji, AU - Tsuji,Shoichiro, AU - Wada,Michihiko, AU - Koizumi,Masayuki, AU - Tulachan,Sidhartha S, AU - Ito,Daisuke, AU - Kami,Kazuhiro, AU - Mori,Tomohiko, AU - Kawaguchi,Yoshiya, AU - Fujimoto,Koji, AU - Hosotani,Ryo, AU - Imamura,Masayuki, PY - 2004/6/26/pubmed PY - 2005/1/4/medline PY - 2004/6/26/entrez SP - 4125 EP - 33 JF - Clinical cancer research : an official journal of the American Association for Cancer Research JO - Clin Cancer Res VL - 10 IS - 12 Pt 1 N2 - PURPOSE: Loss of intercellular adhesion and increased cell motility promote tumor cell invasion. In the present study, E- and N-cadherin, members of the classical cadherin family, are investigated as inducers of epithelial-to-mesenchymal transition (EMT) that is thought to play a fundamental role during the early steps of invasion and metastasis of carcinomas. Cell growth factors are known to regulate cell adhesion molecules. The purpose of the study presented here was to investigate whether a gain in N-cadherin in pancreatic cancer is involved in the process of metastasis via EMT and whether its expression is affected by growth factors. EXPERIMENTAL DESIGN: We immunohistochemically examined the expression of N- and E-cadherins and vimentin, a mesenchymal marker, in pancreatic primary and metastatic tumors. Correlations among the expressions of N-cadherin, transforming growth factor (TGF)beta, and fibroblast growth factor 2 was evaluated in both tumors, and the induction of cadherin and vimentin by growth factors was examined in cultured cell lines. RESULTS: N-cadherin expression was observed in 13 of 30 primary tumors and in 8 of 15 metastatic tumors. N-cadherin expression correlated with neural invasion (P = 0.008), histological type (P = 0.043), fibroblast growth factor expression in primary tumors (P = 0.007), and TGF expression (P = 0.004) and vimentin (P = 0.01) in metastatic tumors. Vimentin, a mesenchymal marker, was observed in a few cancer cells of primary tumor but was substantially expressed in liver metastasis. TGF stimulated N-cadherin and vimentin protein expression and decreased E-cadherin expression of Panc-1 cells with morphological change. CONCLUSION: This study provided the morphological evidence of EMT in pancreatic carcinoma and revealed that overexpression of N-cadherin is involved in EMT and is affected by growth factors. SN - 1078-0432 UR - https://www.unboundmedicine.com/medline/citation/15217949/N_cadherin_expression_and_epithelial_mesenchymal_transition_in_pancreatic_carcinoma_ L2 - http://clincancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=15217949 DB - PRIME DP - Unbound Medicine ER -