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[Silybum marianum in vitro].
Ceska Slov Farm 2004; 53(3):135-40CS

Abstract

Callus and suspension cultures of Silybum marianum were derived and the growth and production characteristics were assayed. The maximal production of flavonolignans was achieved at the beginning of cultivation, i.e. on day 10 after inoculation in MS medium with alpha-NAA and on day 15 after inoculation in MS medium with 2.4-D with continuous growth of the culture. The content of silymarin in the plant drug was 3.05%, in the in vitro culture it was just tenths of percents. TLC analysis revealed that the spectrum of flavonolignans of the drug differed from the spectrum of flavonolignans produced by tissue cultures. No differences in the spectra of flavonolignans produced by the callus and suspension cultures were observed. The test for antioxidative activity against the DPPH-radical demonstrated marked antioxidative properties of substances produced by the culture of Silybum marianum.

Authors+Show Affiliations

Katedra farmakognozie Farmaceutické fakulty Univerzity Karlovy, Hradec Králové. tumova@faf.cuni.czNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

cze

PubMed ID

15218735

Citation

Tůmová, L, et al. "[Silybum Marianum in Vitro]." Ceska a Slovenska Farmacie : Casopis Ceske Farmaceuticke Spolecnosti a Slovenske Farmaceuticke Spolecnosti, vol. 53, no. 3, 2004, pp. 135-40.
Tůmová L, Gallová K, Rimáková J. [Silybum marianum in vitro]. Ceska Slov Farm. 2004;53(3):135-40.
Tůmová, L., Gallová, K., & Rimáková, J. (2004). [Silybum marianum in vitro]. Ceska a Slovenska Farmacie : Casopis Ceske Farmaceuticke Spolecnosti a Slovenske Farmaceuticke Spolecnosti, 53(3), pp. 135-40.
Tůmová L, Gallová K, Rimáková J. [Silybum Marianum in Vitro]. Ceska Slov Farm. 2004;53(3):135-40. PubMed PMID: 15218735.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Silybum marianum in vitro]. AU - Tůmová,L, AU - Gallová,K, AU - Rimáková,J, PY - 2004/6/29/pubmed PY - 2004/7/23/medline PY - 2004/6/29/entrez SP - 135 EP - 40 JF - Ceska a Slovenska farmacie : casopis Ceske farmaceuticke spolecnosti a Slovenske farmaceuticke spolecnosti JO - Ceska Slov Farm VL - 53 IS - 3 N2 - Callus and suspension cultures of Silybum marianum were derived and the growth and production characteristics were assayed. The maximal production of flavonolignans was achieved at the beginning of cultivation, i.e. on day 10 after inoculation in MS medium with alpha-NAA and on day 15 after inoculation in MS medium with 2.4-D with continuous growth of the culture. The content of silymarin in the plant drug was 3.05%, in the in vitro culture it was just tenths of percents. TLC analysis revealed that the spectrum of flavonolignans of the drug differed from the spectrum of flavonolignans produced by tissue cultures. No differences in the spectra of flavonolignans produced by the callus and suspension cultures were observed. The test for antioxidative activity against the DPPH-radical demonstrated marked antioxidative properties of substances produced by the culture of Silybum marianum. SN - 1210-7816 UR - https://www.unboundmedicine.com/medline/citation/15218735/[Silybum_marianum_in_vitro]_ DB - PRIME DP - Unbound Medicine ER -