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Apoptosis and differential expression of apoptosis-related proteins in endometriotic glandular and stromal cells.
J Soc Gynecol Investig. 2004 Jul; 11(5):318-22.JS

Abstract

OBJECTIVE

Apoptosis is an important regulator of eutopic endometrial function. Endometriosis, the growth of endometrial tissue outside the uterus, could result from increased cellular proliferation or decreased apoptosis in response to appropriate stimuli. The objective of this study was to evaluate the rate of apoptosis and the expression of apoptosis-related Bcl-2 and Bax proteins in endometriotic tissues within the glandular and stromal compartments, according to the phase of the menstrual cycle and the stage of disease.

METHODS

Ovarian endometriosis samples were evaluated in 75 women who had surgery at a university hospital. Apoptotic cells were detected with the use of the dUTP nick-end labeling (TUNEL) assay. Bcl-2 and Bax expression were assessed by immunohistochemical techniques.

RESULTS

The percentage of apoptotic cells was significantly higher in endometriotic stromal cells (73.3%) compared with glandular cells (48%; P =.002). In contrast, the expression of the apoptosis-related proteins Bcl-2 and Bax was significantly lower in the endometriotic stroma (17.3% for both) than in the glandular epithelium (38.6% and 41.3%, respectively; P <.004). No significant menstrual cycle phase-dependent changes or endometriosis stage-related changes were observed in TUNEL, Bcl-2, or Bax positivity within ovarian endometriotic tissues.

CONCLUSION

Apoptosis occurs in ovarian endometriotic lesions at significantly higher levels in the stroma than the glandular epithelium. However, Bcl-2 and Bax proteins are distributed preferentially in glandular epithelial cells. The apoptotic rate as well as Bcl-2 and Bax expression in ovarian endometriotic cells were not affected by the stage of endometriosis or the phase of the menstrual cycle.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520-8063, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15219886

Citation

Goumenou, Anastasia G., et al. "Apoptosis and Differential Expression of Apoptosis-related Proteins in Endometriotic Glandular and Stromal Cells." Journal of the Society for Gynecologic Investigation, vol. 11, no. 5, 2004, pp. 318-22.
Goumenou AG, Matalliotakis IM, Tzardi M, et al. Apoptosis and differential expression of apoptosis-related proteins in endometriotic glandular and stromal cells. J Soc Gynecol Investig. 2004;11(5):318-22.
Goumenou, A. G., Matalliotakis, I. M., Tzardi, M., Fragouli, Y. G., Mahutte, N. G., & Arici, A. (2004). Apoptosis and differential expression of apoptosis-related proteins in endometriotic glandular and stromal cells. Journal of the Society for Gynecologic Investigation, 11(5), 318-22.
Goumenou AG, et al. Apoptosis and Differential Expression of Apoptosis-related Proteins in Endometriotic Glandular and Stromal Cells. J Soc Gynecol Investig. 2004;11(5):318-22. PubMed PMID: 15219886.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Apoptosis and differential expression of apoptosis-related proteins in endometriotic glandular and stromal cells. AU - Goumenou,Anastasia G, AU - Matalliotakis,Ioannis M, AU - Tzardi,Maria, AU - Fragouli,Yvoni G, AU - Mahutte,Neal G, AU - Arici,Aydin, PY - 2004/6/29/pubmed PY - 2005/3/8/medline PY - 2004/6/29/entrez SP - 318 EP - 22 JF - Journal of the Society for Gynecologic Investigation JO - J. Soc. Gynecol. Investig. VL - 11 IS - 5 N2 - OBJECTIVE: Apoptosis is an important regulator of eutopic endometrial function. Endometriosis, the growth of endometrial tissue outside the uterus, could result from increased cellular proliferation or decreased apoptosis in response to appropriate stimuli. The objective of this study was to evaluate the rate of apoptosis and the expression of apoptosis-related Bcl-2 and Bax proteins in endometriotic tissues within the glandular and stromal compartments, according to the phase of the menstrual cycle and the stage of disease. METHODS: Ovarian endometriosis samples were evaluated in 75 women who had surgery at a university hospital. Apoptotic cells were detected with the use of the dUTP nick-end labeling (TUNEL) assay. Bcl-2 and Bax expression were assessed by immunohistochemical techniques. RESULTS: The percentage of apoptotic cells was significantly higher in endometriotic stromal cells (73.3%) compared with glandular cells (48%; P =.002). In contrast, the expression of the apoptosis-related proteins Bcl-2 and Bax was significantly lower in the endometriotic stroma (17.3% for both) than in the glandular epithelium (38.6% and 41.3%, respectively; P <.004). No significant menstrual cycle phase-dependent changes or endometriosis stage-related changes were observed in TUNEL, Bcl-2, or Bax positivity within ovarian endometriotic tissues. CONCLUSION: Apoptosis occurs in ovarian endometriotic lesions at significantly higher levels in the stroma than the glandular epithelium. However, Bcl-2 and Bax proteins are distributed preferentially in glandular epithelial cells. The apoptotic rate as well as Bcl-2 and Bax expression in ovarian endometriotic cells were not affected by the stage of endometriosis or the phase of the menstrual cycle. SN - 1071-5576 UR - https://www.unboundmedicine.com/medline/citation/15219886/Apoptosis_and_differential_expression_of_apoptosis_related_proteins_in_endometriotic_glandular_and_stromal_cells_ DB - PRIME DP - Unbound Medicine ER -