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Silibinin inhibits the invasion of human lung cancer cells via decreased productions of urokinase-plasminogen activator and matrix metalloproteinase-2.
Mol Carcinog. 2004 Jul; 40(3):143-9.MC

Abstract

Cancer metastasis, involving multiple processes and various cytophysiological changes, is a primary cause of cancer death and may complicate the clinical management, even lead to death. Silibinin is a flavonoid antioxidant and wildly used for its antihepatotoxic properties and recent studies have revealed pleiotropic anticancer and antiproliferative capabilities of silibinin. In this study, we first observed that silibinin exerted a dose- and time-dependent inhibitory effect on the invasion and motility, but hardly on the adhesion, of highly metastatic A549 cells in the absence of cytotoxicity. To look at the precise involvement of silibinin in cancer metastasis, A549 cells were treated with silibinin at various concentrations, up to 100 microM, for a defined period and then subjected to gelatin zymography, casein zymography and Western blot to investigate the impacts of silibinin on metalloproteinase-2 (MMP-2), urokinase plasminogen activator (u-PA), and tissue inhibitor of metalloproteinase-2 (TIMP-2), respectively. The results showed that a silibinin treatment may decrease the expressions of MMP-2 and u-PA in a concentration- and time-dependent manner and enhance the expression of TIMP-2. Further analysis with semi-quantitative RT-PCR showed that silibinin may regulate the expressions of MMP-2 and u-PA on the transcriptional level while on the translational or post-translational level for TIMP-2.

Authors+Show Affiliations

Department of Food Science, Chungtai Institute of Health Sciences and Technology, Taichung, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15224346

Citation

Chu, Shu-Chen, et al. "Silibinin Inhibits the Invasion of Human Lung Cancer Cells Via Decreased Productions of Urokinase-plasminogen Activator and Matrix Metalloproteinase-2." Molecular Carcinogenesis, vol. 40, no. 3, 2004, pp. 143-9.
Chu SC, Chiou HL, Chen PN, et al. Silibinin inhibits the invasion of human lung cancer cells via decreased productions of urokinase-plasminogen activator and matrix metalloproteinase-2. Mol Carcinog. 2004;40(3):143-9.
Chu, S. C., Chiou, H. L., Chen, P. N., Yang, S. F., & Hsieh, Y. S. (2004). Silibinin inhibits the invasion of human lung cancer cells via decreased productions of urokinase-plasminogen activator and matrix metalloproteinase-2. Molecular Carcinogenesis, 40(3), 143-9.
Chu SC, et al. Silibinin Inhibits the Invasion of Human Lung Cancer Cells Via Decreased Productions of Urokinase-plasminogen Activator and Matrix Metalloproteinase-2. Mol Carcinog. 2004;40(3):143-9. PubMed PMID: 15224346.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Silibinin inhibits the invasion of human lung cancer cells via decreased productions of urokinase-plasminogen activator and matrix metalloproteinase-2. AU - Chu,Shu-Chen, AU - Chiou,Hui-Ling, AU - Chen,Pei-Ni, AU - Yang,Shun-Fa, AU - Hsieh,Yih-Shou, PY - 2004/6/30/pubmed PY - 2004/8/11/medline PY - 2004/6/30/entrez SP - 143 EP - 9 JF - Molecular carcinogenesis JO - Mol Carcinog VL - 40 IS - 3 N2 - Cancer metastasis, involving multiple processes and various cytophysiological changes, is a primary cause of cancer death and may complicate the clinical management, even lead to death. Silibinin is a flavonoid antioxidant and wildly used for its antihepatotoxic properties and recent studies have revealed pleiotropic anticancer and antiproliferative capabilities of silibinin. In this study, we first observed that silibinin exerted a dose- and time-dependent inhibitory effect on the invasion and motility, but hardly on the adhesion, of highly metastatic A549 cells in the absence of cytotoxicity. To look at the precise involvement of silibinin in cancer metastasis, A549 cells were treated with silibinin at various concentrations, up to 100 microM, for a defined period and then subjected to gelatin zymography, casein zymography and Western blot to investigate the impacts of silibinin on metalloproteinase-2 (MMP-2), urokinase plasminogen activator (u-PA), and tissue inhibitor of metalloproteinase-2 (TIMP-2), respectively. The results showed that a silibinin treatment may decrease the expressions of MMP-2 and u-PA in a concentration- and time-dependent manner and enhance the expression of TIMP-2. Further analysis with semi-quantitative RT-PCR showed that silibinin may regulate the expressions of MMP-2 and u-PA on the transcriptional level while on the translational or post-translational level for TIMP-2. SN - 0899-1987 UR - https://www.unboundmedicine.com/medline/citation/15224346/Silibinin_inhibits_the_invasion_of_human_lung_cancer_cells_via_decreased_productions_of_urokinase_plasminogen_activator_and_matrix_metalloproteinase_2_ L2 - https://doi.org/10.1002/mc.20018 DB - PRIME DP - Unbound Medicine ER -