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Hfq is essential for Vibrio cholerae virulence and downregulates sigma expression.
Mol Microbiol. 2004 Jul; 53(1):345-54.MM

Abstract

Hfq is an RNA-binding protein that interacts with both small untranslated RNAs (sRNAs) and mRNAs to modulate gene expression post-transcriptionally. In Escherichia coli and Salmonella typhimurium, Hfq is required for efficient expression of the stationary phase sigma factor sigma(S), and consequently is critical for Salmonella virulence. We have found that Hfq is also essential for the virulence of Vibrio cholerae, as strains lacking hfq fail to colonize the suckling mouse intestine. Deletion of the V. cholerae hfq does not prevent production of sigma(S), nor does it prevent expression of TCP, V. cholerae's primary colonization factor. The expression and activity of the alternative sigma factor sigma(E) are dramatically increased in a V. cholerae hfq mutant. Comparison of the transcriptome of an hfq mutant with that of an rseA mutant, which also overexpresses sigma(E), revealed that sigma(E) controls approximately half the genes found to be upregulated in the hfq mutant. However, increased sigma(E) does not appear to account for this strain's reduced virulence. It is likely that sRNAs, in conjunction with Hfq, are critical regulators of V. cholerae pathogenicity.

Authors+Show Affiliations

Howard Hughes Medical Institute and Tufts University School of Medicine, 136 Harrison Ave., Boston, MA 02111, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15225327

Citation

Ding, Yanpeng, et al. "Hfq Is Essential for Vibrio Cholerae Virulence and Downregulates Sigma Expression." Molecular Microbiology, vol. 53, no. 1, 2004, pp. 345-54.
Ding Y, Davis BM, Waldor MK. Hfq is essential for Vibrio cholerae virulence and downregulates sigma expression. Mol Microbiol. 2004;53(1):345-54.
Ding, Y., Davis, B. M., & Waldor, M. K. (2004). Hfq is essential for Vibrio cholerae virulence and downregulates sigma expression. Molecular Microbiology, 53(1), 345-54.
Ding Y, Davis BM, Waldor MK. Hfq Is Essential for Vibrio Cholerae Virulence and Downregulates Sigma Expression. Mol Microbiol. 2004;53(1):345-54. PubMed PMID: 15225327.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hfq is essential for Vibrio cholerae virulence and downregulates sigma expression. AU - Ding,Yanpeng, AU - Davis,Brigid M, AU - Waldor,Matthew K, PY - 2004/7/1/pubmed PY - 2004/12/16/medline PY - 2004/7/1/entrez SP - 345 EP - 54 JF - Molecular microbiology JO - Mol Microbiol VL - 53 IS - 1 N2 - Hfq is an RNA-binding protein that interacts with both small untranslated RNAs (sRNAs) and mRNAs to modulate gene expression post-transcriptionally. In Escherichia coli and Salmonella typhimurium, Hfq is required for efficient expression of the stationary phase sigma factor sigma(S), and consequently is critical for Salmonella virulence. We have found that Hfq is also essential for the virulence of Vibrio cholerae, as strains lacking hfq fail to colonize the suckling mouse intestine. Deletion of the V. cholerae hfq does not prevent production of sigma(S), nor does it prevent expression of TCP, V. cholerae's primary colonization factor. The expression and activity of the alternative sigma factor sigma(E) are dramatically increased in a V. cholerae hfq mutant. Comparison of the transcriptome of an hfq mutant with that of an rseA mutant, which also overexpresses sigma(E), revealed that sigma(E) controls approximately half the genes found to be upregulated in the hfq mutant. However, increased sigma(E) does not appear to account for this strain's reduced virulence. It is likely that sRNAs, in conjunction with Hfq, are critical regulators of V. cholerae pathogenicity. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/15225327/Hfq_is_essential_for_Vibrio_cholerae_virulence_and_downregulates_sigma_expression_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0950-382X&date=2004&volume=53&issue=1&spage=345 DB - PRIME DP - Unbound Medicine ER -