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Regulation of gene expression of epithelial calcium channels in intestine and kidney of mice by 1alpha,25-dihydroxyvitamin D3.
J Steroid Biochem Mol Biol 2004; 89-90(1-5):335-8JS

Abstract

In wild-type (VDR(+/+)) mice, ECaC2 expression was confirmed in the intestine and kidney, while ECaC1 expression was exclusively confined to the kidney. Both mRNAs expression of ECaC1 and ECaC2 in the kidney and ECaC2 mRNA expression in the intestine increased time- and dose-dependently in response to 1alpha,25(OH)(2)D(3) injection in VDR(+/+) mice, but not in VDR(-/-) mice. The mRNA levels of ECaC2 in the intestine of VDR(-/-) mice were remarkably reduced when compared to VDR(+/+) mice, while no significant differences were observed in both mRNA levels of ECaC1 and ECaC2 in the kidney between VDR(+/+) mice and VDR(-/-) mice. In the primary renal tubular cells (PRTC) isolated from VDR(+/+) mice, both ECaCs mRNA expression increased in response to 1alpha,25(OH)(2)D(3) treatment, but not in the PRTC of VDR(-/-) mice. PTH increased both ECaCs mRNA expression in the PRTC of VDR(+/+) mice. These results suggest that 1alpha,25(OH)(2)D(3) directly modulates the gene expression of ECaC1 and ECaC2 together with PTH in the kidney of mice. 1alpha,25(OH)(2)D(3) also modulates the gene expression of ECaC2 in the intestine of mice, however, further studies are needed to elucidate the direct action of 1alpha,25(OH)(2)D(3) on the expression of ECaC2 in the intestine.

Authors+Show Affiliations

Department of Hygienic Sciences, Kobe Pharmaceutical University, 4-19-1 Motoyamakita-machi, Higashinada-ku, Kobe 658-8558, Japan. t-okano@kobepharma-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15225796

Citation

Okano, Toshio, et al. "Regulation of Gene Expression of Epithelial Calcium Channels in Intestine and Kidney of Mice By 1alpha,25-dihydroxyvitamin D3." The Journal of Steroid Biochemistry and Molecular Biology, vol. 89-90, no. 1-5, 2004, pp. 335-8.
Okano T, Tsugawa N, Morishita A, et al. Regulation of gene expression of epithelial calcium channels in intestine and kidney of mice by 1alpha,25-dihydroxyvitamin D3. J Steroid Biochem Mol Biol. 2004;89-90(1-5):335-8.
Okano, T., Tsugawa, N., Morishita, A., & Kato, S. (2004). Regulation of gene expression of epithelial calcium channels in intestine and kidney of mice by 1alpha,25-dihydroxyvitamin D3. The Journal of Steroid Biochemistry and Molecular Biology, 89-90(1-5), pp. 335-8.
Okano T, et al. Regulation of Gene Expression of Epithelial Calcium Channels in Intestine and Kidney of Mice By 1alpha,25-dihydroxyvitamin D3. J Steroid Biochem Mol Biol. 2004;89-90(1-5):335-8. PubMed PMID: 15225796.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of gene expression of epithelial calcium channels in intestine and kidney of mice by 1alpha,25-dihydroxyvitamin D3. AU - Okano,Toshio, AU - Tsugawa,Naoko, AU - Morishita,Atsushi, AU - Kato,Shigeaki, PY - 2004/7/1/pubmed PY - 2004/9/1/medline PY - 2004/7/1/entrez SP - 335 EP - 8 JF - The Journal of steroid biochemistry and molecular biology JO - J. Steroid Biochem. Mol. Biol. VL - 89-90 IS - 1-5 N2 - In wild-type (VDR(+/+)) mice, ECaC2 expression was confirmed in the intestine and kidney, while ECaC1 expression was exclusively confined to the kidney. Both mRNAs expression of ECaC1 and ECaC2 in the kidney and ECaC2 mRNA expression in the intestine increased time- and dose-dependently in response to 1alpha,25(OH)(2)D(3) injection in VDR(+/+) mice, but not in VDR(-/-) mice. The mRNA levels of ECaC2 in the intestine of VDR(-/-) mice were remarkably reduced when compared to VDR(+/+) mice, while no significant differences were observed in both mRNA levels of ECaC1 and ECaC2 in the kidney between VDR(+/+) mice and VDR(-/-) mice. In the primary renal tubular cells (PRTC) isolated from VDR(+/+) mice, both ECaCs mRNA expression increased in response to 1alpha,25(OH)(2)D(3) treatment, but not in the PRTC of VDR(-/-) mice. PTH increased both ECaCs mRNA expression in the PRTC of VDR(+/+) mice. These results suggest that 1alpha,25(OH)(2)D(3) directly modulates the gene expression of ECaC1 and ECaC2 together with PTH in the kidney of mice. 1alpha,25(OH)(2)D(3) also modulates the gene expression of ECaC2 in the intestine of mice, however, further studies are needed to elucidate the direct action of 1alpha,25(OH)(2)D(3) on the expression of ECaC2 in the intestine. SN - 0960-0760 UR - https://www.unboundmedicine.com/medline/citation/15225796/Regulation_of_gene_expression_of_epithelial_calcium_channels_in_intestine_and_kidney_of_mice_by_1alpha25_dihydroxyvitamin_D3_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S096007600400072X DB - PRIME DP - Unbound Medicine ER -