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Water-soluble polysaccharide from Eleutherococcus senticosus stems attenuates fulminant hepatic failure induced by D-galactosamine and lipopolysaccharide in mice.

Abstract

The aim of this study was to investigate whether Eleutherococcus senticosus stems could attenuate D-galactosamine/lipopolysaccharide-induced fulminant hepatic failure in mice. E. senticosus, known as Siberian ginseng, is a popular folk medicine used as a tonic in Asia. Preparations of E. senticosus used in this study were as follows; (i) 70% ethanol extract (ii) water extract (iii) ethanol-soluble part of the water extract (iv) polysaccharide obtained as an 80% ethanol insoluble of the water extract. Preparations were given by intraperitoneal (300 mg/kg and 50 mg/kg) or oral (300 mg/kg) injection at 12 hr and 1 hr before a D-galactosamine/lipopolysaccharide injection. The intraperitoneal injection of water extract and polysaccharide significantly lowered serum levels of tumour necrosis factor-alpha, aspartate transaminase and alanine transaminase, improved the histologic changes in liver, inhibited hepatocyte apoptosis confirmed by the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling method and DNA fragmentation assay, and suppressed the lethality induced by D-galactosamine/lipopolysaccharide. The oral administration of water extract and polysaccharide also reduced serum aspartate transaminase, alanine transaminase and tumour necrosis factor-alpha levels. In contrast 70% ethanol extract and ethanol-soluble part of the water extract had no protective effect when treated intraperitoneally or orally. These results indicate E. senticosus stems attenuate fulminant hepatic failure induced by D-galactosamine/lipopolysaccharide in mice and the protective effect is due to water-soluble polysaccharides in E. senticosus stems.

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  • Authors+Show Affiliations

    ,

    Department of Pharmacy, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea.

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    Source

    MeSH

    Alanine Transaminase
    Animals
    Apoptosis
    Aspartate Aminotransferases
    DNA Fragmentation
    Eleutherococcus
    Galactosamine
    Hepatocytes
    Lipopolysaccharides
    Liver
    Liver Failure
    Male
    Mice
    Mice, Inbred Strains
    Phytotherapy
    Plant Extracts
    Plant Stems
    Polysaccharides
    Protective Agents
    Solubility
    Tumor Necrosis Factor-alpha

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    15228502

    Citation

    Park, Eun-Jeon, et al. "Water-soluble Polysaccharide From Eleutherococcus Senticosus Stems Attenuates Fulminant Hepatic Failure Induced By D-galactosamine and Lipopolysaccharide in Mice." Basic & Clinical Pharmacology & Toxicology, vol. 94, no. 6, 2004, pp. 298-304.
    Park EJ, Nan JX, Zhao YZ, et al. Water-soluble polysaccharide from Eleutherococcus senticosus stems attenuates fulminant hepatic failure induced by D-galactosamine and lipopolysaccharide in mice. Basic Clin Pharmacol Toxicol. 2004;94(6):298-304.
    Park, E. J., Nan, J. X., Zhao, Y. Z., Lee, S. H., Kim, Y. H., Nam, J. B., ... Sohn, D. H. (2004). Water-soluble polysaccharide from Eleutherococcus senticosus stems attenuates fulminant hepatic failure induced by D-galactosamine and lipopolysaccharide in mice. Basic & Clinical Pharmacology & Toxicology, 94(6), pp. 298-304.
    Park EJ, et al. Water-soluble Polysaccharide From Eleutherococcus Senticosus Stems Attenuates Fulminant Hepatic Failure Induced By D-galactosamine and Lipopolysaccharide in Mice. Basic Clin Pharmacol Toxicol. 2004;94(6):298-304. PubMed PMID: 15228502.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Water-soluble polysaccharide from Eleutherococcus senticosus stems attenuates fulminant hepatic failure induced by D-galactosamine and lipopolysaccharide in mice. AU - Park,Eun-Jeon, AU - Nan,Ji-Xing, AU - Zhao,Yu-Zhe, AU - Lee,Sung Hee, AU - Kim,Young Ho, AU - Nam,Jeong Bum, AU - Lee,Jung Joon, AU - Sohn,Dong Hwan, PY - 2004/7/2/pubmed PY - 2004/10/23/medline PY - 2004/7/2/entrez SP - 298 EP - 304 JF - Basic & clinical pharmacology & toxicology JO - Basic Clin. Pharmacol. Toxicol. VL - 94 IS - 6 N2 - The aim of this study was to investigate whether Eleutherococcus senticosus stems could attenuate D-galactosamine/lipopolysaccharide-induced fulminant hepatic failure in mice. E. senticosus, known as Siberian ginseng, is a popular folk medicine used as a tonic in Asia. Preparations of E. senticosus used in this study were as follows; (i) 70% ethanol extract (ii) water extract (iii) ethanol-soluble part of the water extract (iv) polysaccharide obtained as an 80% ethanol insoluble of the water extract. Preparations were given by intraperitoneal (300 mg/kg and 50 mg/kg) or oral (300 mg/kg) injection at 12 hr and 1 hr before a D-galactosamine/lipopolysaccharide injection. The intraperitoneal injection of water extract and polysaccharide significantly lowered serum levels of tumour necrosis factor-alpha, aspartate transaminase and alanine transaminase, improved the histologic changes in liver, inhibited hepatocyte apoptosis confirmed by the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling method and DNA fragmentation assay, and suppressed the lethality induced by D-galactosamine/lipopolysaccharide. The oral administration of water extract and polysaccharide also reduced serum aspartate transaminase, alanine transaminase and tumour necrosis factor-alpha levels. In contrast 70% ethanol extract and ethanol-soluble part of the water extract had no protective effect when treated intraperitoneally or orally. These results indicate E. senticosus stems attenuate fulminant hepatic failure induced by D-galactosamine/lipopolysaccharide in mice and the protective effect is due to water-soluble polysaccharides in E. senticosus stems. SN - 1742-7835 UR - https://www.unboundmedicine.com/medline/citation/15228502/Water_soluble_polysaccharide_from_Eleutherococcus_senticosus_stems_attenuates_fulminant_hepatic_failure_induced_by_D_galactosamine_and_lipopolysaccharide_in_mice_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=1742-7835&date=2004&volume=94&issue=6&spage=298 DB - PRIME DP - Unbound Medicine ER -