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Purification of paeoniflorin from Paeonia lactiflora Pall. by high-speed counter-current chromatography.
J Chromatogr A. 2004 Jun 25; 1040(2):205-8.JC

Abstract

High-speed counter-current chromatography was successfully used for the first time for the preparative separation and purification of paeoniflorin from the Chinese medicinal plant Paeonia lactiflora Pall. using a two-phase solvent system composed of n-butanol-ethyl acetate-water (1:4:5, v/v) in a single run. From 160 mg of the crude sample containing 22.0% paeoniflorin, 33.2 mg of paeoniflorin was yielded at 98.2% purity as determined by HPLC analysis. The recovery of paeoniflorin was 94.3%.

Authors+Show Affiliations

Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong, China. sfchen@hkusua.hku.hkNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15230527

Citation

Chen, Feng, et al. "Purification of Paeoniflorin From Paeonia Lactiflora Pall. By High-speed Counter-current Chromatography." Journal of Chromatography. A, vol. 1040, no. 2, 2004, pp. 205-8.
Chen F, Lu HT, Jiang Y. Purification of paeoniflorin from Paeonia lactiflora Pall. by high-speed counter-current chromatography. J Chromatogr A. 2004;1040(2):205-8.
Chen, F., Lu, H. T., & Jiang, Y. (2004). Purification of paeoniflorin from Paeonia lactiflora Pall. by high-speed counter-current chromatography. Journal of Chromatography. A, 1040(2), 205-8.
Chen F, Lu HT, Jiang Y. Purification of Paeoniflorin From Paeonia Lactiflora Pall. By High-speed Counter-current Chromatography. J Chromatogr A. 2004 Jun 25;1040(2):205-8. PubMed PMID: 15230527.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Purification of paeoniflorin from Paeonia lactiflora Pall. by high-speed counter-current chromatography. AU - Chen,Feng, AU - Lu,Hai-Tao, AU - Jiang,Yue, PY - 2004/7/3/pubmed PY - 2005/2/25/medline PY - 2004/7/3/entrez SP - 205 EP - 8 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1040 IS - 2 N2 - High-speed counter-current chromatography was successfully used for the first time for the preparative separation and purification of paeoniflorin from the Chinese medicinal plant Paeonia lactiflora Pall. using a two-phase solvent system composed of n-butanol-ethyl acetate-water (1:4:5, v/v) in a single run. From 160 mg of the crude sample containing 22.0% paeoniflorin, 33.2 mg of paeoniflorin was yielded at 98.2% purity as determined by HPLC analysis. The recovery of paeoniflorin was 94.3%. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/15230527/Purification_of_paeoniflorin_from_Paeonia_lactiflora_Pall__by_high_speed_counter_current_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(04)00602-8 DB - PRIME DP - Unbound Medicine ER -