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The Bacillus subtilis yqjI gene encodes the NADP+-dependent 6-P-gluconate dehydrogenase in the pentose phosphate pathway.
J Bacteriol. 2004 Jul; 186(14):4528-34.JB

Abstract

Despite the importance of the oxidative pentose phosphate (PP) pathway as a major source of reducing power and metabolic intermediates for biosynthetic processes, almost no direct genetic or biochemical evidence is available for Bacillus subtilis. Using a combination of knockout mutations in known and putative genes of the oxidative PP pathway and 13C-labeling experiments, we demonstrated that yqjI encodes the NADP+-dependent 6-P-gluconate dehydrogenase, as was hypothesized previously from sequence similarities. Moreover, YqjI was the predominant isoenzyme during glucose and gluconate catabolism, and its role in the oxidative PP pathway could not be played by either of two homologues, GntZ and YqeC. This conclusion is in contrast to the generally held view that GntZ is the relevant isoform; hence, we propose a new designation for yqjI, gndA, the monocistronic gene encoding the principal 6-P-gluconate dehydrogenase. Although we demonstrated the NAD+-dependent 6-P-gluconate dehydrogenase activity of GntZ, gntZ mutants exhibited no detectable phenotype on glucose, and GntZ did not contribute to PP pathway fluxes during growth on glucose. Since gntZ mutants grew normally on gluconate, the functional role of GntZ remains obscure, as does the role of the third homologue, YqeC. Knockout of the glucose-6-P dehydrogenase-encoding zwf gene was primarily compensated for by increased glycolytic fluxes, but about 5% of the catabolic flux was rerouted through the gluconate bypass with glucose dehydrogenase as the key enzyme.

Authors+Show Affiliations

Institute of Biotechnology, ETH Zürich, Zürich, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15231785

Citation

Zamboni, Nicola, et al. "The Bacillus Subtilis yqjI Gene Encodes the NADP+-dependent 6-P-gluconate Dehydrogenase in the Pentose Phosphate Pathway." Journal of Bacteriology, vol. 186, no. 14, 2004, pp. 4528-34.
Zamboni N, Fischer E, Laudert D, et al. The Bacillus subtilis yqjI gene encodes the NADP+-dependent 6-P-gluconate dehydrogenase in the pentose phosphate pathway. J Bacteriol. 2004;186(14):4528-34.
Zamboni, N., Fischer, E., Laudert, D., Aymerich, S., Hohmann, H. P., & Sauer, U. (2004). The Bacillus subtilis yqjI gene encodes the NADP+-dependent 6-P-gluconate dehydrogenase in the pentose phosphate pathway. Journal of Bacteriology, 186(14), 4528-34.
Zamboni N, et al. The Bacillus Subtilis yqjI Gene Encodes the NADP+-dependent 6-P-gluconate Dehydrogenase in the Pentose Phosphate Pathway. J Bacteriol. 2004;186(14):4528-34. PubMed PMID: 15231785.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Bacillus subtilis yqjI gene encodes the NADP+-dependent 6-P-gluconate dehydrogenase in the pentose phosphate pathway. AU - Zamboni,Nicola, AU - Fischer,Eliane, AU - Laudert,Dietmar, AU - Aymerich,Stéphane, AU - Hohmann,Hans-Peter, AU - Sauer,Uwe, PY - 2004/7/3/pubmed PY - 2004/8/24/medline PY - 2004/7/3/entrez SP - 4528 EP - 34 JF - Journal of bacteriology JO - J. Bacteriol. VL - 186 IS - 14 N2 - Despite the importance of the oxidative pentose phosphate (PP) pathway as a major source of reducing power and metabolic intermediates for biosynthetic processes, almost no direct genetic or biochemical evidence is available for Bacillus subtilis. Using a combination of knockout mutations in known and putative genes of the oxidative PP pathway and 13C-labeling experiments, we demonstrated that yqjI encodes the NADP+-dependent 6-P-gluconate dehydrogenase, as was hypothesized previously from sequence similarities. Moreover, YqjI was the predominant isoenzyme during glucose and gluconate catabolism, and its role in the oxidative PP pathway could not be played by either of two homologues, GntZ and YqeC. This conclusion is in contrast to the generally held view that GntZ is the relevant isoform; hence, we propose a new designation for yqjI, gndA, the monocistronic gene encoding the principal 6-P-gluconate dehydrogenase. Although we demonstrated the NAD+-dependent 6-P-gluconate dehydrogenase activity of GntZ, gntZ mutants exhibited no detectable phenotype on glucose, and GntZ did not contribute to PP pathway fluxes during growth on glucose. Since gntZ mutants grew normally on gluconate, the functional role of GntZ remains obscure, as does the role of the third homologue, YqeC. Knockout of the glucose-6-P dehydrogenase-encoding zwf gene was primarily compensated for by increased glycolytic fluxes, but about 5% of the catabolic flux was rerouted through the gluconate bypass with glucose dehydrogenase as the key enzyme. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/15231785/The_Bacillus_subtilis_yqjI_gene_encodes_the_NADP+_dependent_6_P_gluconate_dehydrogenase_in_the_pentose_phosphate_pathway_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=15231785 DB - PRIME DP - Unbound Medicine ER -