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A comprehensive expression analysis of the starch synthase gene family in rice (Oryza sativa L.).
Planta. 2004 Nov; 220(1):9-16.P

Abstract

To elucidate the roles of the isogenes encoding starch synthase (EC 2.4.1.21) in rice (Oryza sativa L.), a comprehensive expression analysis of the gene family was conducted. Extensive searches for starch synthase genes were done in the databases of both the whole genome and full-length cDNAs of rice, and ten genes were revealed to comprise the starch synthase gene family. Multi-sequence alignment analysis of the starch synthase proteins from rice and other plant species suggested that they were grouped into five classes, soluble starch synthase I (SSI), SSII, SSIII, SSIV and granule-bound starch synthase (GBSS). In rice, there was one gene for SSI, three for SSII and two each for SSIII, IV and GBSS. The expression pattern of the ten genes in the developing caryopsis was examined by semi-quantitative RT-PCR analysis. Based on the temporal expression patterns, the ten genes could be divided into three groups: (i) early expressers (SSII-2, III-1, GBSSII), which are expressed in the early stage of grain filling; (ii) late expressers (SSII-3, III-2, GBSSI), which are expressed in the mid to later stage of grain filling; and (iii) steady expressers (SSI, II-1, IV-1, IV-2), which are expressed relatively constantly during grain filling. Within a caryopsis, the three gene groups spatially share their expression, i.e. "early expressers" in the pericarp, the "late expressers" in the endosperm" and the "steady expressers" in both tissues. In addition, this grouping was reflected in the expression pattern of various rice tissues: expression in non-endosperm, endosperm or all tissues examined. The implications in this spatio-temporal work sharing of starch synthesis isogenes are discussed.

Authors+Show Affiliations

Hirose T
Department of Rice Research, National Agricultural Research Center, Joetsu, 943-0193 Niigata, Japan. dragon@affrc.go.jp
Terao T
No affiliation info available

MeSH

DNA PrimersDNA, ComplementaryGenome, PlantIsoenzymesMultigene FamilyOryzaPhylogenyReverse Transcriptase Polymerase Chain ReactionStarch Synthase

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15232694

Citation

Hirose, Tatsuro, and Tomio Terao. "A Comprehensive Expression Analysis of the Starch Synthase Gene Family in Rice (Oryza Sativa L.)." Planta, vol. 220, no. 1, 2004, pp. 9-16.
Hirose T, Terao T. A comprehensive expression analysis of the starch synthase gene family in rice (Oryza sativa L.). Planta. 2004;220(1):9-16.
Hirose, T., & Terao, T. (2004). A comprehensive expression analysis of the starch synthase gene family in rice (Oryza sativa L.). Planta, 220(1), 9-16.
Hirose T, Terao T. A Comprehensive Expression Analysis of the Starch Synthase Gene Family in Rice (Oryza Sativa L.). Planta. 2004;220(1):9-16. PubMed PMID: 15232694.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A comprehensive expression analysis of the starch synthase gene family in rice (Oryza sativa L.). AU - Hirose,Tatsuro, AU - Terao,Tomio, Y1 - 2004/06/30/ PY - 2004/01/20/received PY - 2004/05/05/accepted PY - 2004/7/3/pubmed PY - 2005/5/13/medline PY - 2004/7/3/entrez SP - 9 EP - 16 JF - Planta JO - Planta VL - 220 IS - 1 N2 - To elucidate the roles of the isogenes encoding starch synthase (EC 2.4.1.21) in rice (Oryza sativa L.), a comprehensive expression analysis of the gene family was conducted. Extensive searches for starch synthase genes were done in the databases of both the whole genome and full-length cDNAs of rice, and ten genes were revealed to comprise the starch synthase gene family. Multi-sequence alignment analysis of the starch synthase proteins from rice and other plant species suggested that they were grouped into five classes, soluble starch synthase I (SSI), SSII, SSIII, SSIV and granule-bound starch synthase (GBSS). In rice, there was one gene for SSI, three for SSII and two each for SSIII, IV and GBSS. The expression pattern of the ten genes in the developing caryopsis was examined by semi-quantitative RT-PCR analysis. Based on the temporal expression patterns, the ten genes could be divided into three groups: (i) early expressers (SSII-2, III-1, GBSSII), which are expressed in the early stage of grain filling; (ii) late expressers (SSII-3, III-2, GBSSI), which are expressed in the mid to later stage of grain filling; and (iii) steady expressers (SSI, II-1, IV-1, IV-2), which are expressed relatively constantly during grain filling. Within a caryopsis, the three gene groups spatially share their expression, i.e. "early expressers" in the pericarp, the "late expressers" in the endosperm" and the "steady expressers" in both tissues. In addition, this grouping was reflected in the expression pattern of various rice tissues: expression in non-endosperm, endosperm or all tissues examined. The implications in this spatio-temporal work sharing of starch synthesis isogenes are discussed. SN - 0032-0935 UR - https://www.unboundmedicine.com/medline/citation/15232694/A_comprehensive_expression_analysis_of_the_starch_synthase_gene_family_in_rice__Oryza_sativa_L___ DB - PRIME DP - Unbound Medicine ER -