Tags

Type your tag names separated by a space and hit enter

The spatial and temporal pattern of fatty acid amide hydrolase expression in rat hippocampus during postnatal development.

Abstract

GABAergic synaptic transmission is efficiently controlled by endogenous cannabinoids in cortical structures. Fatty acid amide hydrolase (FAAH) is one of the metabolizing enzymes of endocannabinoids in the brain. In this study we investigated the cellular and subcellular distribution of FAAH at various timepoints during the first postnatal weeks, when GABA is still depolarizing, and plays a crucial role in network events. FAAH immunoreactivity is strong in the CA3 region already at postnatal day 0 (P0), but in CA1 only after P8. During this period, FAAH levels in hilar mossy cells decrease and in granule cells slowly increase. Pyramidal cells express FAAH first in the soma and proximal dendrites, and gradually in more distal segments, reaching adult levels in the most distal dendrites only at P22. Transient expression of FAAH was found in a small number of stratum radiatum cells that may be interneurons and in ependymal cells at the border of the alveus and corpus callosum between P2 and P8. At the ultrastructural level, FAAH distribution at all ages was very similar to the adult pattern, i.e. it was largely associated with the membrane of cytoplasmic vesicles, mitochondria and endoplasmic reticulum. During postnatal development of the hippocampus, the spatio-temporal expression of FAAH correlates well with the general pattern of neuronal maturation, but not with the arrival of afferent pathways, which suggests that FAAH - and its major endocannabinoid substrate, anandamide - is unlikely to be involved in the presynaptic control of neurotransmission. Instead, FAAH may subserve general roles as the inactivating enzyme for many fatty acid amides, in addition to anandamide.

Links

  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Institute of Experimental Medicine, Hungarian Academy of Sciences, Szigony utca 43, H-1083, Budapest, Hungary. yury.morozov@yale.edu

    ,

    Source

    The European journal of neuroscience 20:2 2004 Jul pg 459-66

    MeSH

    Age Factors
    Amidohydrolases
    Animals
    Animals, Newborn
    Gene Expression Regulation, Developmental
    Hippocampus
    Immunohistochemistry
    Male
    Microscopy, Immunoelectron
    Neurons
    Rats
    Rats, Wistar

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't
    Research Support, U.S. Gov't, P.H.S.

    Language

    eng

    PubMed ID

    15233754

    Citation

    Morozov, Yury M., et al. "The Spatial and Temporal Pattern of Fatty Acid Amide Hydrolase Expression in Rat Hippocampus During Postnatal Development." The European Journal of Neuroscience, vol. 20, no. 2, 2004, pp. 459-66.
    Morozov YM, Ben-Ari Y, Freund TF. The spatial and temporal pattern of fatty acid amide hydrolase expression in rat hippocampus during postnatal development. Eur J Neurosci. 2004;20(2):459-66.
    Morozov, Y. M., Ben-Ari, Y., & Freund, T. F. (2004). The spatial and temporal pattern of fatty acid amide hydrolase expression in rat hippocampus during postnatal development. The European Journal of Neuroscience, 20(2), pp. 459-66.
    Morozov YM, Ben-Ari Y, Freund TF. The Spatial and Temporal Pattern of Fatty Acid Amide Hydrolase Expression in Rat Hippocampus During Postnatal Development. Eur J Neurosci. 2004;20(2):459-66. PubMed PMID: 15233754.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - The spatial and temporal pattern of fatty acid amide hydrolase expression in rat hippocampus during postnatal development. AU - Morozov,Yury M, AU - Ben-Ari,Yezekiel, AU - Freund,Tamas F, PY - 2004/7/6/pubmed PY - 2004/10/1/medline PY - 2004/7/6/entrez SP - 459 EP - 66 JF - The European journal of neuroscience JO - Eur. J. Neurosci. VL - 20 IS - 2 N2 - GABAergic synaptic transmission is efficiently controlled by endogenous cannabinoids in cortical structures. Fatty acid amide hydrolase (FAAH) is one of the metabolizing enzymes of endocannabinoids in the brain. In this study we investigated the cellular and subcellular distribution of FAAH at various timepoints during the first postnatal weeks, when GABA is still depolarizing, and plays a crucial role in network events. FAAH immunoreactivity is strong in the CA3 region already at postnatal day 0 (P0), but in CA1 only after P8. During this period, FAAH levels in hilar mossy cells decrease and in granule cells slowly increase. Pyramidal cells express FAAH first in the soma and proximal dendrites, and gradually in more distal segments, reaching adult levels in the most distal dendrites only at P22. Transient expression of FAAH was found in a small number of stratum radiatum cells that may be interneurons and in ependymal cells at the border of the alveus and corpus callosum between P2 and P8. At the ultrastructural level, FAAH distribution at all ages was very similar to the adult pattern, i.e. it was largely associated with the membrane of cytoplasmic vesicles, mitochondria and endoplasmic reticulum. During postnatal development of the hippocampus, the spatio-temporal expression of FAAH correlates well with the general pattern of neuronal maturation, but not with the arrival of afferent pathways, which suggests that FAAH - and its major endocannabinoid substrate, anandamide - is unlikely to be involved in the presynaptic control of neurotransmission. Instead, FAAH may subserve general roles as the inactivating enzyme for many fatty acid amides, in addition to anandamide. SN - 0953-816X UR - https://www.unboundmedicine.com/medline/citation/15233754/The_spatial_and_temporal_pattern_of_fatty_acid_amide_hydrolase_expression_in_rat_hippocampus_during_postnatal_development_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0953-816X&date=2004&volume=20&issue=2&spage=459 DB - PRIME DP - Unbound Medicine ER -