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Molecular biology of extended-spectrum beta-lactamase-producing Enterobacteriaceae responsible for digestive tract colonization.
J Hosp Infect. 2004 Jul; 57(3):202-8.JH

Abstract

Twenty-nine extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae strains (14 Klebsiella pneumoniae, 10 Escherichia coli and five Citrobacter diversus) isolated from April to July 1996 from faecal carriers in a surgical intensive care unit at the university hospital of Casablanca (Morocco) were studied. Plasmid content and DNA macrorestriction polymorphism determined by pulsed-field gel electrophoresis (PFGE) were used to compare the strains. Restriction profiles of total genomic DNAs cleaved by XbaI and compared by PFGE revealed nine, four and two clones in K. pneumoniae, E. coli and C. diversus, respectively. Plasmid profile analysis of ESBL-producing strains of K. pneumoniae showed that only seven of 14 isolates had a plasmid; four different plasmid profiles were observed. Three different plasmid profiles were observed in E. coli and two in C. diversus. Plasmids responsible for ESBL production could be transferred by conjugation to E. coli K(12) J53-2 from all E. coli isolates and from four of seven K. pneumoniae. No plasmid transfer could be obtained from C. diversus strains. Restriction enzyme digests of plasmids from transconjugants (four transconjugants of K. pneumoniae and five transconjugants of E. coli) showed different patterns. In the surgical intensive care unit where the survey was conducted, the dissemination of ESBLs was due to a mix of strain spread and strain diversity rather than to plasmid dissemination.

Authors+Show Affiliations

Microbiology laboratory, IbnRochd University Hospital, Casablanca, Morocco.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15236848

Citation

Moustaoui, N, et al. "Molecular Biology of Extended-spectrum Beta-lactamase-producing Enterobacteriaceae Responsible for Digestive Tract Colonization." The Journal of Hospital Infection, vol. 57, no. 3, 2004, pp. 202-8.
Moustaoui N, Soukri A, Elmdaghri N, et al. Molecular biology of extended-spectrum beta-lactamase-producing Enterobacteriaceae responsible for digestive tract colonization. J Hosp Infect. 2004;57(3):202-8.
Moustaoui, N., Soukri, A., Elmdaghri, N., Boudouma, M., & Benbachir, M. (2004). Molecular biology of extended-spectrum beta-lactamase-producing Enterobacteriaceae responsible for digestive tract colonization. The Journal of Hospital Infection, 57(3), 202-8.
Moustaoui N, et al. Molecular Biology of Extended-spectrum Beta-lactamase-producing Enterobacteriaceae Responsible for Digestive Tract Colonization. J Hosp Infect. 2004;57(3):202-8. PubMed PMID: 15236848.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular biology of extended-spectrum beta-lactamase-producing Enterobacteriaceae responsible for digestive tract colonization. AU - Moustaoui,N, AU - Soukri,A, AU - Elmdaghri,N, AU - Boudouma,M, AU - Benbachir,M, PY - 2003/10/29/received PY - 2004/01/08/accepted PY - 2004/7/9/pubmed PY - 2004/10/9/medline PY - 2004/7/9/entrez SP - 202 EP - 8 JF - The Journal of hospital infection JO - J. Hosp. Infect. VL - 57 IS - 3 N2 - Twenty-nine extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae strains (14 Klebsiella pneumoniae, 10 Escherichia coli and five Citrobacter diversus) isolated from April to July 1996 from faecal carriers in a surgical intensive care unit at the university hospital of Casablanca (Morocco) were studied. Plasmid content and DNA macrorestriction polymorphism determined by pulsed-field gel electrophoresis (PFGE) were used to compare the strains. Restriction profiles of total genomic DNAs cleaved by XbaI and compared by PFGE revealed nine, four and two clones in K. pneumoniae, E. coli and C. diversus, respectively. Plasmid profile analysis of ESBL-producing strains of K. pneumoniae showed that only seven of 14 isolates had a plasmid; four different plasmid profiles were observed. Three different plasmid profiles were observed in E. coli and two in C. diversus. Plasmids responsible for ESBL production could be transferred by conjugation to E. coli K(12) J53-2 from all E. coli isolates and from four of seven K. pneumoniae. No plasmid transfer could be obtained from C. diversus strains. Restriction enzyme digests of plasmids from transconjugants (four transconjugants of K. pneumoniae and five transconjugants of E. coli) showed different patterns. In the surgical intensive care unit where the survey was conducted, the dissemination of ESBLs was due to a mix of strain spread and strain diversity rather than to plasmid dissemination. SN - 0195-6701 UR - https://www.unboundmedicine.com/medline/citation/15236848/Molecular_biology_of_extended_spectrum_beta_lactamase_producing_Enterobacteriaceae_responsible_for_digestive_tract_colonization_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0195670104000155 DB - PRIME DP - Unbound Medicine ER -