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[Protective effect of specific antibody in serum of convalescent patient with SARS].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2004 Jul; 16(7):409-12.ZW

Abstract

OBJECTIVE

To investigate inhibitory effect of serum severe acute respiratory syndrome (SARS) -specific antibodies from convalescent patients after half an year of onset on SARS-CoV-mediated cytopathic response.

METHODS

SARS-CoV immunoglobulin G (IgG) antibody was determined by enzyme linked immunoadsorbent assay (ELISA). Twelve serum samples from convalescent patients, diluted by 1:8 with maintenance medium, were mixed with the three dilution supernatants of SARS-CoV. SARS-CoV were isolated, cultured and identified by the Guangzhou Institute of Respiratory Disease, and cultured with Vero E6 cell suspension. The extent of cytopathic response was observed.

RESULTS

The absorbance (A) value of SARS-CoV IgG antibody ranged from 0.81 to 2.06 in patients after half an year of SARS onset, and form 0.79 to 2.01 in patients before half an year of SARS onset. The extent of cytopathic response was decreased by more than 25% in all 12 convalescent patients, as compared with control serum.

CONCLUSION

The A value of SARS-CoV IgG antibody in serum of convalescent patients tended to elevate in half an year after SARS onset. SARS-CoV IgG antibody could inhibit SARS-CoV-mediated cytopathic response, indicating it might be one of protective antibodies.

Authors+Show Affiliations

Li Y
Guangzhou Institute of Respiratory Disease, Guangzhou 510120, Guangdong, China. liyong999@163.net
Xu J
No affiliation info available
Mo HY
No affiliation info available
Lai KF
No affiliation info available
Tan YX
No affiliation info available
Pi XY
No affiliation info available
Ren XL
No affiliation info available
Zeng GQ
No affiliation info available
Liu MF
No affiliation info available
Zhong NS
No affiliation info available

MeSH

AdultAntibodies, ViralFemaleHumansImmunoglobulin GMaleMiddle AgedNeutralization TestsSevere acute respiratory syndrome-related coronavirusSevere Acute Respiratory Syndrome

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15238177

Citation

Li, Yong, et al. "[Protective Effect of Specific Antibody in Serum of Convalescent Patient With SARS]." Zhongguo Wei Zhong Bing Ji Jiu Yi Xue = Chinese Critical Care Medicine = Zhongguo Weizhongbing Jijiuyixue, vol. 16, no. 7, 2004, pp. 409-12.
Li Y, Xu J, Mo HY, et al. [Protective effect of specific antibody in serum of convalescent patient with SARS]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2004;16(7):409-12.
Li, Y., Xu, J., Mo, H. Y., Lai, K. F., Tan, Y. X., Pi, X. Y., Ren, X. L., Zeng, G. Q., Liu, M. F., & Zhong, N. S. (2004). [Protective effect of specific antibody in serum of convalescent patient with SARS]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue = Chinese Critical Care Medicine = Zhongguo Weizhongbing Jijiuyixue, 16(7), 409-12.
Li Y, et al. [Protective Effect of Specific Antibody in Serum of Convalescent Patient With SARS]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2004;16(7):409-12. PubMed PMID: 15238177.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Protective effect of specific antibody in serum of convalescent patient with SARS]. AU - Li,Yong, AU - Xu,Jun, AU - Mo,Hong-ying, AU - Lai,Ke-fang, AU - Tan,Ya-xia, AU - Pi,Xiang-ying, AU - Ren,Xiao-Lan, AU - Zeng,Guang-qiao, AU - Liu,Miao-Fang, AU - Zhong,Nan-shan, PY - 2004/7/9/pubmed PY - 2009/9/9/medline PY - 2004/7/9/entrez SP - 409 EP - 12 JF - Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue JO - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue VL - 16 IS - 7 N2 - OBJECTIVE: To investigate inhibitory effect of serum severe acute respiratory syndrome (SARS) -specific antibodies from convalescent patients after half an year of onset on SARS-CoV-mediated cytopathic response. METHODS: SARS-CoV immunoglobulin G (IgG) antibody was determined by enzyme linked immunoadsorbent assay (ELISA). Twelve serum samples from convalescent patients, diluted by 1:8 with maintenance medium, were mixed with the three dilution supernatants of SARS-CoV. SARS-CoV were isolated, cultured and identified by the Guangzhou Institute of Respiratory Disease, and cultured with Vero E6 cell suspension. The extent of cytopathic response was observed. RESULTS: The absorbance (A) value of SARS-CoV IgG antibody ranged from 0.81 to 2.06 in patients after half an year of SARS onset, and form 0.79 to 2.01 in patients before half an year of SARS onset. The extent of cytopathic response was decreased by more than 25% in all 12 convalescent patients, as compared with control serum. CONCLUSION: The A value of SARS-CoV IgG antibody in serum of convalescent patients tended to elevate in half an year after SARS onset. SARS-CoV IgG antibody could inhibit SARS-CoV-mediated cytopathic response, indicating it might be one of protective antibodies. SN - 1003-0603 UR - https://www.unboundmedicine.com/medline/citation/15238177/[Protective_effect_of_specific_antibody_in_serum_of_convalescent_patient_with_SARS]_ DB - PRIME DP - Unbound Medicine ER -