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Sequence polymorphism in polyploid wheat and their d-genome diploid ancestor.
Genetics. 2004 Jun; 167(2):941-7.G

Abstract

Sequencing was used to investigate the origin of the D genome of the allopolyploid species Triticum aestivum and Aegilops cylindrica. A 247-bp region of the wheat D-genome Xwye838 locus, encoding ADP-glucopyrophosphorylase, and a 326-bp region of the wheat D-genome Gss locus, encoding granule-bound starch synthase, were sequenced in a total 564 lines of hexaploid wheat (T. aestivum, genome AABBDD) involving all its subspecies and 203 lines of Aegilops tauschii, the diploid source of the wheat D genome. In Ae. tauschii, two SNP variants were detected at the Xwye838 locus and 11 haplotypes at the Gss locus. Two haplotypes with contrasting frequencies were found at each locus in wheat. Both wheat Xwye838 variants, but only one of the Gss haplotypes seen in wheat, were found among the Ae. tauschii lines. The other wheat Gss haplotype was not found in either Ae. tauschii or 70 lines of tetraploid Ae. cylindrica (genomes CCDD), which is known to hybridize with wheat. It is concluded that both T. aestivum and Ae. cylindrica originated recurrently, with at least two genetically distinct progenitors contributing to the formation of the D genome in both species.

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Authors+Show Affiliations

Caldwell KS
E. I. DuPont Nemours, DuPont Agricultural Products, Newark, Delaware 19711, USA.
Dvorak J
No affiliation info available
Lagudah ES
No affiliation info available
Akhunov E
No affiliation info available
Luo MC
No affiliation info available
Wolters P
No affiliation info available
Powell W
No affiliation info available

MeSH

Base SequenceDNA PrimersDiploidyGenetic VariationGenome, PlantNucleic Acid ConformationPolymerase Chain ReactionPolyploidySequence AlignmentTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15238542

Citation

Caldwell, Katherine S., et al. "Sequence Polymorphism in Polyploid Wheat and Their D-genome Diploid Ancestor." Genetics, vol. 167, no. 2, 2004, pp. 941-7.
Caldwell KS, Dvorak J, Lagudah ES, et al. Sequence polymorphism in polyploid wheat and their d-genome diploid ancestor. Genetics. 2004;167(2):941-7.
Caldwell, K. S., Dvorak, J., Lagudah, E. S., Akhunov, E., Luo, M. C., Wolters, P., & Powell, W. (2004). Sequence polymorphism in polyploid wheat and their d-genome diploid ancestor. Genetics, 167(2), 941-7.
Caldwell KS, et al. Sequence Polymorphism in Polyploid Wheat and Their D-genome Diploid Ancestor. Genetics. 2004;167(2):941-7. PubMed PMID: 15238542.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sequence polymorphism in polyploid wheat and their d-genome diploid ancestor. AU - Caldwell,Katherine S, AU - Dvorak,Jan, AU - Lagudah,Evans S, AU - Akhunov,Eduard, AU - Luo,Ming-Cheng, AU - Wolters,Petra, AU - Powell,Wayne, PY - 2004/7/9/pubmed PY - 2005/4/27/medline PY - 2004/7/9/entrez SP - 941 EP - 7 JF - Genetics JO - Genetics VL - 167 IS - 2 N2 - Sequencing was used to investigate the origin of the D genome of the allopolyploid species Triticum aestivum and Aegilops cylindrica. A 247-bp region of the wheat D-genome Xwye838 locus, encoding ADP-glucopyrophosphorylase, and a 326-bp region of the wheat D-genome Gss locus, encoding granule-bound starch synthase, were sequenced in a total 564 lines of hexaploid wheat (T. aestivum, genome AABBDD) involving all its subspecies and 203 lines of Aegilops tauschii, the diploid source of the wheat D genome. In Ae. tauschii, two SNP variants were detected at the Xwye838 locus and 11 haplotypes at the Gss locus. Two haplotypes with contrasting frequencies were found at each locus in wheat. Both wheat Xwye838 variants, but only one of the Gss haplotypes seen in wheat, were found among the Ae. tauschii lines. The other wheat Gss haplotype was not found in either Ae. tauschii or 70 lines of tetraploid Ae. cylindrica (genomes CCDD), which is known to hybridize with wheat. It is concluded that both T. aestivum and Ae. cylindrica originated recurrently, with at least two genetically distinct progenitors contributing to the formation of the D genome in both species. SN - 0016-6731 UR - https://www.unboundmedicine.com/medline/citation/15238542/Sequence_polymorphism_in_polyploid_wheat_and_their_d_genome_diploid_ancestor_ DB - PRIME DP - Unbound Medicine ER -