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Evaluation of the One-Step ELISA kit for the detection of buprenorphine in urine, blood, and hair specimens.
Forensic Sci Int. 2004 Jul 16; 143(2-3):153-6.FS

Abstract

A solid-phase enzyme immunoassay involving microtiter plates was recently proposed by International Diagnostic Systems corporation (IDS) to screen for buprenorphine in human serum. The performance of the kit led us to investigate its applicability in other biological matrices such as urine or blood, and also hair specimens. Low concentrations of buprenorphine were detected with the ELISA test and confirmed by HPLC/MS (buprenorphine concentrations measured by HPLC/MS: 0.3 ng/mL in urine, 0.2 ng/mL in blood, and 40 pg/mg in hair). The intra-assay precision values were 8.7% at 1 ng/mL of urine (n = 8), 11.5% at 2 ng/mL in serum (n = 8), and 11.5% at 250 pg/mg of hair (n = 8), respectively. The immunoassay had no cross-reactivity with dihydrocodeine, ethylmorphine, 6-monoacetylmorphine, pholcodine, propoxyphene, dextromoramide, dextrometorphan at 1 and 10 mg/L, or codeine, morphine, methadone, and its metabolite EDDP. A 1% cross-reactivity was measured for a norbuprenorphine concentration of 50 ng/mL. Finally, the immunoassay was validated by comparing authentic specimens results with those of a validated HPLC/MS method. From the 136 urine samples tested, 93 were positive (68.4%) after the ELISA screening test (cutoff: 0.5 ng/mL) and confirmed by HPLC/MS (buprenorphine concentrations: 0.3-2036 ng/mL). From the 108 blood or serum samples screened, 27 were positive (25%) after the ELISA test with a cutoff value of 0.5 ng/mL (buprenorphine concentrations: 0.2-13.3 ng/mL). Eighteen hair specimens were positive (72%) after the screening (cutoff: 10 pg/mg) and confirmed by LC/MS (buprenorphine concentrations: 40-360 pg/mg). The ELISA method produced false positive results in less than 21% of the cases, but no false negative results were observed with the immunological test. Four potential adulterants (hypochloride 50 mL/L, sodium nitrite 50 g/L, liquid soap 50 mL/L, and sodium chloride 50 g/L) that were added to 10 positive urine specimens (buprenorphine concentrations in the range 5.3-15.6 ng/mL), did not cause a false negative response by the immunoassay.

Authors+Show Affiliations

Laboratoire de Toxicologie, Institut de Médecine Légale, 11 rue Humann, F-67085 Strasbourg, France. vincent.cirimele@medecine.u-strasbg.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

15240036

Citation

Cirimele, V, et al. "Evaluation of the One-Step ELISA Kit for the Detection of Buprenorphine in Urine, Blood, and Hair Specimens." Forensic Science International, vol. 143, no. 2-3, 2004, pp. 153-6.
Cirimele V, Etienne S, Villain M, et al. Evaluation of the One-Step ELISA kit for the detection of buprenorphine in urine, blood, and hair specimens. Forensic Sci Int. 2004;143(2-3):153-6.
Cirimele, V., Etienne, S., Villain, M., Ludes, B., & Kintz, P. (2004). Evaluation of the One-Step ELISA kit for the detection of buprenorphine in urine, blood, and hair specimens. Forensic Science International, 143(2-3), 153-6.
Cirimele V, et al. Evaluation of the One-Step ELISA Kit for the Detection of Buprenorphine in Urine, Blood, and Hair Specimens. Forensic Sci Int. 2004 Jul 16;143(2-3):153-6. PubMed PMID: 15240036.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the One-Step ELISA kit for the detection of buprenorphine in urine, blood, and hair specimens. AU - Cirimele,V, AU - Etienne,S, AU - Villain,M, AU - Ludes,B, AU - Kintz,P, PY - 2003/11/20/received PY - 2004/02/20/accepted PY - 2004/7/9/pubmed PY - 2004/11/2/medline PY - 2004/7/9/entrez SP - 153 EP - 6 JF - Forensic science international JO - Forensic Sci. Int. VL - 143 IS - 2-3 N2 - A solid-phase enzyme immunoassay involving microtiter plates was recently proposed by International Diagnostic Systems corporation (IDS) to screen for buprenorphine in human serum. The performance of the kit led us to investigate its applicability in other biological matrices such as urine or blood, and also hair specimens. Low concentrations of buprenorphine were detected with the ELISA test and confirmed by HPLC/MS (buprenorphine concentrations measured by HPLC/MS: 0.3 ng/mL in urine, 0.2 ng/mL in blood, and 40 pg/mg in hair). The intra-assay precision values were 8.7% at 1 ng/mL of urine (n = 8), 11.5% at 2 ng/mL in serum (n = 8), and 11.5% at 250 pg/mg of hair (n = 8), respectively. The immunoassay had no cross-reactivity with dihydrocodeine, ethylmorphine, 6-monoacetylmorphine, pholcodine, propoxyphene, dextromoramide, dextrometorphan at 1 and 10 mg/L, or codeine, morphine, methadone, and its metabolite EDDP. A 1% cross-reactivity was measured for a norbuprenorphine concentration of 50 ng/mL. Finally, the immunoassay was validated by comparing authentic specimens results with those of a validated HPLC/MS method. From the 136 urine samples tested, 93 were positive (68.4%) after the ELISA screening test (cutoff: 0.5 ng/mL) and confirmed by HPLC/MS (buprenorphine concentrations: 0.3-2036 ng/mL). From the 108 blood or serum samples screened, 27 were positive (25%) after the ELISA test with a cutoff value of 0.5 ng/mL (buprenorphine concentrations: 0.2-13.3 ng/mL). Eighteen hair specimens were positive (72%) after the screening (cutoff: 10 pg/mg) and confirmed by LC/MS (buprenorphine concentrations: 40-360 pg/mg). The ELISA method produced false positive results in less than 21% of the cases, but no false negative results were observed with the immunological test. Four potential adulterants (hypochloride 50 mL/L, sodium nitrite 50 g/L, liquid soap 50 mL/L, and sodium chloride 50 g/L) that were added to 10 positive urine specimens (buprenorphine concentrations in the range 5.3-15.6 ng/mL), did not cause a false negative response by the immunoassay. SN - 0379-0738 UR - https://www.unboundmedicine.com/medline/citation/15240036/Evaluation_of_the_One_Step_ELISA_kit_for_the_detection_of_buprenorphine_in_urine_blood_and_hair_specimens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0379073804002105 DB - PRIME DP - Unbound Medicine ER -