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Miniaturized sample preparation method for determination of amphetamines in urine.
Forensic Sci Int. 2004 Jul 16; 143(2-3):163-7.FS

Abstract

A simple and miniaturized sample preparation method for determination of amphetamines in urine was developed using on-column derivatization and gas chromatography-mass spectrometry (GC-MS). Urine was directly applied to the extraction column that was pre-packed with Extrelut and sodium carbonate. Amphetamine (AP) and methamphetamine (MA) in urine were adsorbed on the surface of Extrelut. AP and MA were then converted to a free base and derivatized to N-propoxycarbonyl derivatives using propylchloroformate on the column. Pentadeuterated MA was used as an internal standard. The recoveries of AP and MA from urine were 100 and 102%, respectively. The calibration curves showed linearity in the range of 0.50-50 microg/mL for AP and MA in urine. When urine samples containing two different concentrations (0.50 and 5.0 microg/mL) of AP and MA were determined, the intra-day and inter-day coefficients of variation were 1.4-7.7%. This method was applied to 14 medico-legal cases of MA intoxication. The results were compared and a good agreement was obtained with a HPLC method.

Authors+Show Affiliations

Department of Legal Medicine, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, 734-8551, Japan. nishidam@hiroshima-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15240038

Citation

Nishida, Manami, et al. "Miniaturized Sample Preparation Method for Determination of Amphetamines in Urine." Forensic Science International, vol. 143, no. 2-3, 2004, pp. 163-7.
Nishida M, Namera A, Yashiki M, et al. Miniaturized sample preparation method for determination of amphetamines in urine. Forensic Sci Int. 2004;143(2-3):163-7.
Nishida, M., Namera, A., Yashiki, M., & Kimura, K. (2004). Miniaturized sample preparation method for determination of amphetamines in urine. Forensic Science International, 143(2-3), 163-7.
Nishida M, et al. Miniaturized Sample Preparation Method for Determination of Amphetamines in Urine. Forensic Sci Int. 2004 Jul 16;143(2-3):163-7. PubMed PMID: 15240038.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Miniaturized sample preparation method for determination of amphetamines in urine. AU - Nishida,Manami, AU - Namera,Akira, AU - Yashiki,Mikio, AU - Kimura,Kojiro, PY - 2003/11/18/received PY - 2004/03/10/accepted PY - 2004/7/9/pubmed PY - 2004/11/2/medline PY - 2004/7/9/entrez SP - 163 EP - 7 JF - Forensic science international JO - Forensic Sci. Int. VL - 143 IS - 2-3 N2 - A simple and miniaturized sample preparation method for determination of amphetamines in urine was developed using on-column derivatization and gas chromatography-mass spectrometry (GC-MS). Urine was directly applied to the extraction column that was pre-packed with Extrelut and sodium carbonate. Amphetamine (AP) and methamphetamine (MA) in urine were adsorbed on the surface of Extrelut. AP and MA were then converted to a free base and derivatized to N-propoxycarbonyl derivatives using propylchloroformate on the column. Pentadeuterated MA was used as an internal standard. The recoveries of AP and MA from urine were 100 and 102%, respectively. The calibration curves showed linearity in the range of 0.50-50 microg/mL for AP and MA in urine. When urine samples containing two different concentrations (0.50 and 5.0 microg/mL) of AP and MA were determined, the intra-day and inter-day coefficients of variation were 1.4-7.7%. This method was applied to 14 medico-legal cases of MA intoxication. The results were compared and a good agreement was obtained with a HPLC method. SN - 0379-0738 UR - https://www.unboundmedicine.com/medline/citation/15240038/Miniaturized_sample_preparation_method_for_determination_of_amphetamines_in_urine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0379073804002129 DB - PRIME DP - Unbound Medicine ER -