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Lactobacillus GG effect in increasing IFN-gamma production in infants with cow's milk allergy.

Abstract

BACKGROUND

Probiotic bacteria are potentially beneficial to maturation of the infant's immune system.

OBJECTIVE

To examine the role of probiotic bacteria in treatment of cow's milk allergy (CMA) and IgE-associated dermatitis, we investigated the immunologic effects of Lactobacillus rhamnosus GG (LGG) and a mixture of 4 bacterial species (MIX).

METHODS

In a randomized, double-blind study design, concomitantly with elimination diet and skin treatment, LGG, MIX, or placebo was given for 4 weeks to infants with suspected CMA. After anti-CD3 (OKT3) and anti-CD28 stimulation of PBMCs, IFN-gamma, IL-4, IL-5, and IL-12 levels were measured in culture supernatants by ELISA. Intracellular IFN-gamma, IL-4, and IL-5 production on CD4 lymphocytes was analyzed with fluorescence-activated cell sorting.

RESULTS

Secretion of IFN-gamma by PBMCs before the treatment was significantly lower in infants with CMA (P=.016) and in infants with IgE-associated CMA (P=.003) than in non-CMA infants. Among the infants who received LGG, the level of secreted IFN-gamma increased in those with CMA (P=.006) and in those with IgE-associated dermatitis (P=.017) when compared with the placebo group. Secretion of IL-4 increased significantly in infants with CMA in the MIX (P=.034) but not in the LGG group.

CONCLUSION

Deficiency in IFN-gamma response appears to be related to CMA. LGG raises IFN-gamma production of PBMC in infants with CMA and in infants with IgE-associated dermatitis and may thus provide beneficial TH1 immunomodulatory signals. MIX, although containing LGG, appears to modulate the immune responses differently.

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  • Authors+Show Affiliations

    ,

    Hospital for Children and Adolescents, University of Helsinki, Finland. emma.pohjavuori@hus.fi

    , , , , ,

    Source

    MeSH

    Cytokines
    Dermatitis, Atopic
    Double-Blind Method
    Female
    Humans
    Immunoglobulin E
    Infant
    Interferon-gamma
    Lactobacillus
    Male
    Milk Hypersensitivity
    Probiotics
    Th1 Cells

    Pub Type(s)

    Clinical Trial
    Journal Article
    Randomized Controlled Trial
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    15241356

    Citation

    Pohjavuori, Emma, et al. "Lactobacillus GG Effect in Increasing IFN-gamma Production in Infants With Cow's Milk Allergy." The Journal of Allergy and Clinical Immunology, vol. 114, no. 1, 2004, pp. 131-6.
    Pohjavuori E, Viljanen M, Korpela R, et al. Lactobacillus GG effect in increasing IFN-gamma production in infants with cow's milk allergy. J Allergy Clin Immunol. 2004;114(1):131-6.
    Pohjavuori, E., Viljanen, M., Korpela, R., Kuitunen, M., Tiittanen, M., Vaarala, O., & Savilahti, E. (2004). Lactobacillus GG effect in increasing IFN-gamma production in infants with cow's milk allergy. The Journal of Allergy and Clinical Immunology, 114(1), pp. 131-6.
    Pohjavuori E, et al. Lactobacillus GG Effect in Increasing IFN-gamma Production in Infants With Cow's Milk Allergy. J Allergy Clin Immunol. 2004;114(1):131-6. PubMed PMID: 15241356.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Lactobacillus GG effect in increasing IFN-gamma production in infants with cow's milk allergy. AU - Pohjavuori,Emma, AU - Viljanen,Mirva, AU - Korpela,Riitta, AU - Kuitunen,Mikael, AU - Tiittanen,Minna, AU - Vaarala,Outi, AU - Savilahti,Erkki, PY - 2004/7/9/pubmed PY - 2004/9/29/medline PY - 2004/7/9/entrez SP - 131 EP - 6 JF - The Journal of allergy and clinical immunology JO - J. Allergy Clin. Immunol. VL - 114 IS - 1 N2 - BACKGROUND: Probiotic bacteria are potentially beneficial to maturation of the infant's immune system. OBJECTIVE: To examine the role of probiotic bacteria in treatment of cow's milk allergy (CMA) and IgE-associated dermatitis, we investigated the immunologic effects of Lactobacillus rhamnosus GG (LGG) and a mixture of 4 bacterial species (MIX). METHODS: In a randomized, double-blind study design, concomitantly with elimination diet and skin treatment, LGG, MIX, or placebo was given for 4 weeks to infants with suspected CMA. After anti-CD3 (OKT3) and anti-CD28 stimulation of PBMCs, IFN-gamma, IL-4, IL-5, and IL-12 levels were measured in culture supernatants by ELISA. Intracellular IFN-gamma, IL-4, and IL-5 production on CD4 lymphocytes was analyzed with fluorescence-activated cell sorting. RESULTS: Secretion of IFN-gamma by PBMCs before the treatment was significantly lower in infants with CMA (P=.016) and in infants with IgE-associated CMA (P=.003) than in non-CMA infants. Among the infants who received LGG, the level of secreted IFN-gamma increased in those with CMA (P=.006) and in those with IgE-associated dermatitis (P=.017) when compared with the placebo group. Secretion of IL-4 increased significantly in infants with CMA in the MIX (P=.034) but not in the LGG group. CONCLUSION: Deficiency in IFN-gamma response appears to be related to CMA. LGG raises IFN-gamma production of PBMC in infants with CMA and in infants with IgE-associated dermatitis and may thus provide beneficial TH1 immunomodulatory signals. MIX, although containing LGG, appears to modulate the immune responses differently. SN - 0091-6749 UR - https://www.unboundmedicine.com/medline/citation/15241356/Lactobacillus_GG_effect_in_increasing_IFN_gamma_production_in_infants_with_cow's_milk_allergy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091674904011686 DB - PRIME DP - Unbound Medicine ER -