Tags

Type your tag names separated by a space and hit enter

Detection of Pneumocystis jiroveci in respiratory specimens by four staining methods.
J Clin Microbiol. 2004 Jul; 42(7):3333-5.JC

Abstract

We examined four staining methods on replicate smears of 313 respiratory specimens submitted for Pneumocystis jiroveci examination. The sensitivity and specificity of Calcofluor white stain (CW) were 73.8 and 99.6%, respectively. The sensitivity and specificity of Grocott-Gomori methenamine silver stain (GMS) were 79.4 and 99.2%, respectively. The sensitivity and specificity of Diff-Quik stain were 49.2 and 99.6%, respectively. The sensitivity and specificity of Merifluor Pneumocystis stain were 90.8 and 81.9%, respectively. Only CW and GMS had positive and negative predictive values of >90%.

Authors+Show Affiliations

Section of Clinical Microbiology, Department of Clinical Pathology, The Cleveland Clinic Foundation, 9500 Euclid Ave., Cleveland, OH 44195, USA. procopg@ccf.orgNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15243109

Citation

Procop, G W., et al. "Detection of Pneumocystis Jiroveci in Respiratory Specimens By Four Staining Methods." Journal of Clinical Microbiology, vol. 42, no. 7, 2004, pp. 3333-5.
Procop GW, Haddad S, Quinn J, et al. Detection of Pneumocystis jiroveci in respiratory specimens by four staining methods. J Clin Microbiol. 2004;42(7):3333-5.
Procop, G. W., Haddad, S., Quinn, J., Wilson, M. L., Henshaw, N. G., Reller, L. B., Artymyshyn, R. L., Katanik, M. T., & Weinstein, M. P. (2004). Detection of Pneumocystis jiroveci in respiratory specimens by four staining methods. Journal of Clinical Microbiology, 42(7), 3333-5.
Procop GW, et al. Detection of Pneumocystis Jiroveci in Respiratory Specimens By Four Staining Methods. J Clin Microbiol. 2004;42(7):3333-5. PubMed PMID: 15243109.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Pneumocystis jiroveci in respiratory specimens by four staining methods. AU - Procop,G W, AU - Haddad,S, AU - Quinn,J, AU - Wilson,M L, AU - Henshaw,N G, AU - Reller,L B, AU - Artymyshyn,R L, AU - Katanik,M T, AU - Weinstein,M P, PY - 2004/7/10/pubmed PY - 2004/8/20/medline PY - 2004/7/10/entrez SP - 3333 EP - 5 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 42 IS - 7 N2 - We examined four staining methods on replicate smears of 313 respiratory specimens submitted for Pneumocystis jiroveci examination. The sensitivity and specificity of Calcofluor white stain (CW) were 73.8 and 99.6%, respectively. The sensitivity and specificity of Grocott-Gomori methenamine silver stain (GMS) were 79.4 and 99.2%, respectively. The sensitivity and specificity of Diff-Quik stain were 49.2 and 99.6%, respectively. The sensitivity and specificity of Merifluor Pneumocystis stain were 90.8 and 81.9%, respectively. Only CW and GMS had positive and negative predictive values of >90%. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/15243109/Detection_of_Pneumocystis_jiroveci_in_respiratory_specimens_by_four_staining_methods_ L2 - https://journals.asm.org/doi/10.1128/JCM.42.7.3333-3335.2004?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -