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Amperometric glucose biosensor based on adsorption of glucose oxidase at platinum nanoparticle-modified carbon nanotube electrode.
Anal Biochem. 2004 Aug 01; 331(1):89-97.AB

Abstract

A new amperometric biosensor, based on adsorption of glucose oxidase (GOD) at the platinum nanoparticle-modified carbon nanotube (CNT) electrode, is presented in this article. CNTs were grown directly on the graphite substrate. The resulting GOD/Pt/CNT electrode was covered by a thin layer of Nafion to avoid the loss of GOD in determination and to improve the anti-interferent ability. The morphologies and electrochemical performance of the CNT, Pt/CNT, and Nafion/GOD/Pt/CNT electrodes have been investigated by scanning electron microscopy, cyclic voltammetry, and amperometric methods. The excellent electrocatalytic activity and special three-dimensional structure of the enzyme electrode result in good characteristics such as a large determination range (0.1-13.5mM), a short response time (within 5s), a large current density (1.176 mA cm(-2)), and high sensitivity (91mA M(-1)cm(-2)) and stability (73.5% remains after 22 days). In addition, effects of pH value, applied potential, electrode construction, and electroactive interferents on the amperometric response of the sensor were investigated and discussed. The reproducibility and applicability to whole blood analysis of the enzyme electrode were also evaluated.

Authors+Show Affiliations

State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15246000

Citation

Tang, Hao, et al. "Amperometric Glucose Biosensor Based On Adsorption of Glucose Oxidase at Platinum Nanoparticle-modified Carbon Nanotube Electrode." Analytical Biochemistry, vol. 331, no. 1, 2004, pp. 89-97.
Tang H, Chen J, Yao S, et al. Amperometric glucose biosensor based on adsorption of glucose oxidase at platinum nanoparticle-modified carbon nanotube electrode. Anal Biochem. 2004;331(1):89-97.
Tang, H., Chen, J., Yao, S., Nie, L., Deng, G., & Kuang, Y. (2004). Amperometric glucose biosensor based on adsorption of glucose oxidase at platinum nanoparticle-modified carbon nanotube electrode. Analytical Biochemistry, 331(1), 89-97.
Tang H, et al. Amperometric Glucose Biosensor Based On Adsorption of Glucose Oxidase at Platinum Nanoparticle-modified Carbon Nanotube Electrode. Anal Biochem. 2004 Aug 1;331(1):89-97. PubMed PMID: 15246000.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amperometric glucose biosensor based on adsorption of glucose oxidase at platinum nanoparticle-modified carbon nanotube electrode. AU - Tang,Hao, AU - Chen,Jinhua, AU - Yao,Shouzhuo, AU - Nie,Lihua, AU - Deng,Guohong, AU - Kuang,Yafei, PY - 2004/01/12/received PY - 2004/7/13/pubmed PY - 2005/1/29/medline PY - 2004/7/13/entrez SP - 89 EP - 97 JF - Analytical biochemistry JO - Anal Biochem VL - 331 IS - 1 N2 - A new amperometric biosensor, based on adsorption of glucose oxidase (GOD) at the platinum nanoparticle-modified carbon nanotube (CNT) electrode, is presented in this article. CNTs were grown directly on the graphite substrate. The resulting GOD/Pt/CNT electrode was covered by a thin layer of Nafion to avoid the loss of GOD in determination and to improve the anti-interferent ability. The morphologies and electrochemical performance of the CNT, Pt/CNT, and Nafion/GOD/Pt/CNT electrodes have been investigated by scanning electron microscopy, cyclic voltammetry, and amperometric methods. The excellent electrocatalytic activity and special three-dimensional structure of the enzyme electrode result in good characteristics such as a large determination range (0.1-13.5mM), a short response time (within 5s), a large current density (1.176 mA cm(-2)), and high sensitivity (91mA M(-1)cm(-2)) and stability (73.5% remains after 22 days). In addition, effects of pH value, applied potential, electrode construction, and electroactive interferents on the amperometric response of the sensor were investigated and discussed. The reproducibility and applicability to whole blood analysis of the enzyme electrode were also evaluated. SN - 0003-2697 UR - https://www.unboundmedicine.com/medline/citation/15246000/Amperometric_glucose_biosensor_based_on_adsorption_of_glucose_oxidase_at_platinum_nanoparticle_modified_carbon_nanotube_electrode_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003269704004117 DB - PRIME DP - Unbound Medicine ER -