Tags

Type your tag names separated by a space and hit enter

Identification of Bacillus anthracis by multiprobe microarray hybridization.
Diagn Microbiol Infect Dis 2004; 49(3):163-71DM

Abstract

We have developed a rapid assay based on microarray analysis of amplified genetic markers for reliable identification of Bacillus anthracis and its discrimination from other closely related bacterial species of the Bacillus cereus group. By combining polymerase chain reaction (PCR) amplification of six B. anthracis-specific genes (plasmid-associated genes encoding virulence factors (cyaA, pagA, lef, and capA, capB, capC) and one chromosomal marker BA-5449) with analysis of amplicons by microarray hybridization, we were able to unambiguously identify and discriminate B. anthracis among other closely related species. Bacillus identification relied on hybridization with multiple individual microarray oligonucleotide probes (oligoprobes) specific to each target B. anthracis gene. Evaluation of the assay was conducted using several B. anthracis strains (with or without pXO1 and pXO2 plasmids) as well as over 50 other species phylogenetically related to B. anthracis, including B. cereus, B. thuringiensis, B. mycoides, and B. subtilis. The developed microarray analysis of amplified genetic markers protocol provides an efficient method for (i) unambiguous identification and discrimination of B. anthracis from other Bacillus species and (ii) distinguishing between plasmid-containing and plasmid-free Bacillus anthracis strains.

Authors+Show Affiliations

Center for Biologics Evaluation and Research, Food and Drug Administration, Kensington, MD 20895, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

15246505

Citation

Volokhov, Dmitriy, et al. "Identification of Bacillus Anthracis By Multiprobe Microarray Hybridization." Diagnostic Microbiology and Infectious Disease, vol. 49, no. 3, 2004, pp. 163-71.
Volokhov D, Pomerantsev A, Kivovich V, et al. Identification of Bacillus anthracis by multiprobe microarray hybridization. Diagn Microbiol Infect Dis. 2004;49(3):163-71.
Volokhov, D., Pomerantsev, A., Kivovich, V., Rasooly, A., & Chizhikov, V. (2004). Identification of Bacillus anthracis by multiprobe microarray hybridization. Diagnostic Microbiology and Infectious Disease, 49(3), pp. 163-71.
Volokhov D, et al. Identification of Bacillus Anthracis By Multiprobe Microarray Hybridization. Diagn Microbiol Infect Dis. 2004;49(3):163-71. PubMed PMID: 15246505.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of Bacillus anthracis by multiprobe microarray hybridization. AU - Volokhov,Dmitriy, AU - Pomerantsev,Andrei, AU - Kivovich,Violetta, AU - Rasooly,Avraham, AU - Chizhikov,Vladimir, PY - 2003/11/03/received PY - 2004/03/22/accepted PY - 2004/7/13/pubmed PY - 2004/10/6/medline PY - 2004/7/13/entrez SP - 163 EP - 71 JF - Diagnostic microbiology and infectious disease JO - Diagn. Microbiol. Infect. Dis. VL - 49 IS - 3 N2 - We have developed a rapid assay based on microarray analysis of amplified genetic markers for reliable identification of Bacillus anthracis and its discrimination from other closely related bacterial species of the Bacillus cereus group. By combining polymerase chain reaction (PCR) amplification of six B. anthracis-specific genes (plasmid-associated genes encoding virulence factors (cyaA, pagA, lef, and capA, capB, capC) and one chromosomal marker BA-5449) with analysis of amplicons by microarray hybridization, we were able to unambiguously identify and discriminate B. anthracis among other closely related species. Bacillus identification relied on hybridization with multiple individual microarray oligonucleotide probes (oligoprobes) specific to each target B. anthracis gene. Evaluation of the assay was conducted using several B. anthracis strains (with or without pXO1 and pXO2 plasmids) as well as over 50 other species phylogenetically related to B. anthracis, including B. cereus, B. thuringiensis, B. mycoides, and B. subtilis. The developed microarray analysis of amplified genetic markers protocol provides an efficient method for (i) unambiguous identification and discrimination of B. anthracis from other Bacillus species and (ii) distinguishing between plasmid-containing and plasmid-free Bacillus anthracis strains. SN - 0732-8893 UR - https://www.unboundmedicine.com/medline/citation/15246505/Identification_of_Bacillus_anthracis_by_multiprobe_microarray_hybridization_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732889304000598 DB - PRIME DP - Unbound Medicine ER -