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Analysis of benzyldimethyldodecylammonium bromide in chemical disinfectants by liquid chromatography and capillary electrophoresis.
J Chromatogr A. 2004 Jun 11; 1039(1-2):209-13.JC

Abstract

Two novel analytical methodologies using capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC) were developed and compared for the determination of benzyldimethyldodecylammonium bromide (BAB) in commercial compound chemical disinfectants. The LC analysis was performed with a Kromasil C18 (200 mm x 4.6 mm, 5 microm) column and a mobile phase of A:B = 80:20 (A: acetonitrile, B: 4 mmol/L octanesulfonic sodium--0.02 mol/L acetic sodium, adjusted with acetic acid to pH 5.2) at a flow rate of 1.0 mL/min. Detection was by ultraviolet absorption at 262 nm. The CE analysis was performed in a bare fused-silica capillary with 75 microm i.d. and total length of 46.4 cm with a buffer solution of 50% acetonitrile -50 mmol/L NaH2PO4, pH 2.24. The applied voltage was 20 kV. Detection was by ultraviolet absorption at 214 nm. Under optimized conditions, the HPLC retention time and CE migration time for BAB was 9.18 and 5.08 min, respectively. Calibration curves of peak area versus concentration gave correlation coefficients of 0.9996 for HPLC and 0.9994 for CE. The detection limits for HPLC and CE were 1.6 mg/L and 0.2 mg/L, respectively. Average recoveries at three concentration levels (50, 100, 200 mg/L for HPLC: 20, 40, 100 mg/L for CE) were 99.94 +/- 1.5, 99.64 +/- 1.3 and 99.61 +/- 0.4% for HPLC and 120.47 +/- 2.6, 102.06 +/- 8.7 and 103.05 +/- 3.0% for CE, respectively. Although both methods were shown to be suitable for the determination of BAB in commercial disinfectant compounds, CE provided analysis with less solvent purchase/disposal and better column efficiency, whereas HPLC provided superior precision.

Authors+Show Affiliations

Ding X
Beijing Center for Disease Prevention and Control, Dongcheng District, No. 16, He Pingli Zhongjie, Beijing 100013, China. dxj_wry@yahoo.com
Mou S
No affiliation info available
Zhao S
No affiliation info available

MeSH

Benzalkonium CompoundsChromatography, High Pressure LiquidDisinfectantsElectrophoresis, CapillaryReproducibility of ResultsSensitivity and SpecificitySpectrophotometry, Ultraviolet

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15250425

Citation

Ding, Xiaojing, et al. "Analysis of Benzyldimethyldodecylammonium Bromide in Chemical Disinfectants By Liquid Chromatography and Capillary Electrophoresis." Journal of Chromatography. A, vol. 1039, no. 1-2, 2004, pp. 209-13.
Ding X, Mou S, Zhao S. Analysis of benzyldimethyldodecylammonium bromide in chemical disinfectants by liquid chromatography and capillary electrophoresis. J Chromatogr A. 2004;1039(1-2):209-13.
Ding, X., Mou, S., & Zhao, S. (2004). Analysis of benzyldimethyldodecylammonium bromide in chemical disinfectants by liquid chromatography and capillary electrophoresis. Journal of Chromatography. A, 1039(1-2), 209-13.
Ding X, Mou S, Zhao S. Analysis of Benzyldimethyldodecylammonium Bromide in Chemical Disinfectants By Liquid Chromatography and Capillary Electrophoresis. J Chromatogr A. 2004 Jun 11;1039(1-2):209-13. PubMed PMID: 15250425.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of benzyldimethyldodecylammonium bromide in chemical disinfectants by liquid chromatography and capillary electrophoresis. AU - Ding,Xiaojing, AU - Mou,Shifen, AU - Zhao,Shan, PY - 2004/7/15/pubmed PY - 2005/3/1/medline PY - 2004/7/15/entrez SP - 209 EP - 13 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1039 IS - 1-2 N2 - Two novel analytical methodologies using capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC) were developed and compared for the determination of benzyldimethyldodecylammonium bromide (BAB) in commercial compound chemical disinfectants. The LC analysis was performed with a Kromasil C18 (200 mm x 4.6 mm, 5 microm) column and a mobile phase of A:B = 80:20 (A: acetonitrile, B: 4 mmol/L octanesulfonic sodium--0.02 mol/L acetic sodium, adjusted with acetic acid to pH 5.2) at a flow rate of 1.0 mL/min. Detection was by ultraviolet absorption at 262 nm. The CE analysis was performed in a bare fused-silica capillary with 75 microm i.d. and total length of 46.4 cm with a buffer solution of 50% acetonitrile -50 mmol/L NaH2PO4, pH 2.24. The applied voltage was 20 kV. Detection was by ultraviolet absorption at 214 nm. Under optimized conditions, the HPLC retention time and CE migration time for BAB was 9.18 and 5.08 min, respectively. Calibration curves of peak area versus concentration gave correlation coefficients of 0.9996 for HPLC and 0.9994 for CE. The detection limits for HPLC and CE were 1.6 mg/L and 0.2 mg/L, respectively. Average recoveries at three concentration levels (50, 100, 200 mg/L for HPLC: 20, 40, 100 mg/L for CE) were 99.94 +/- 1.5, 99.64 +/- 1.3 and 99.61 +/- 0.4% for HPLC and 120.47 +/- 2.6, 102.06 +/- 8.7 and 103.05 +/- 3.0% for CE, respectively. Although both methods were shown to be suitable for the determination of BAB in commercial disinfectant compounds, CE provided analysis with less solvent purchase/disposal and better column efficiency, whereas HPLC provided superior precision. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/15250425/Analysis_of_benzyldimethyldodecylammonium_bromide_in_chemical_disinfectants_by_liquid_chromatography_and_capillary_electrophoresis_ DB - PRIME DP - Unbound Medicine ER -