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Molecular anatomy of interendothelial junctions in human blood-brain barrier microvessels.
Folia Histochem Cytobiol. 2004; 42(2):67-75.FH

Abstract

Immunogold cytochemical procedure was used to study the localization at the ultrastructural level of interendothelial junction-associated protein molecules in the human brain blood microvessels, representing the anatomic site of the blood-brain barrier (BBB). Ultrathin sections of Lowicryl K4M-embedded biopsy specimens of human cerebral cortex obtained during surgical procedures were exposed to specific antibodies, followed by colloidal gold-labeled secondary antibodies. All tight junction-specific integral membrane (transmembrane) proteins--occludin, junctional adhesion molecule (JAM-1), and claudin-5--as well as peripheral zonula occludens protein (ZO-1) were highly expressed. Immunoreactivity of the adherens junction-specific transmembrane protein VE-cadherin was of almost similar intensity. Immunolabeling of the adherens junction-associated peripheral proteins--alpha-catenin, beta-catenin, and p120 catenin--although positive, was evidently less intense. The expression of gamma-catenin (plakoglobin) was considered questionable because solitary immunosignals (gold particles) appeared in only a few microvascular profiles. Double labeling of some sections made possible to observe strict colocalization of the junctional molecules, such as occludin and ZO-1 or JAM-1 and VE-cadherin, in the interendothelial junctions. We found that in human brain microvessels, the interendothelial junctional complexes contain molecular components specific for both tight and adherens junctions. It is assumed that the data obtained can help us find the immunodetectable junctional molecules that can serve as sensitive markers of normal or abnormal function of the BBB.

Authors+Show Affiliations

New York State Office of Mental Retardation and Developmental Disabilities, Institute for Basic Research in Developmental Disabilities, Staten Island, New York 10314, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15253128

Citation

Vorbrodt, Andrzej W., and Danuta H. Dobrogowska. "Molecular Anatomy of Interendothelial Junctions in Human Blood-brain Barrier Microvessels." Folia Histochemica Et Cytobiologica, vol. 42, no. 2, 2004, pp. 67-75.
Vorbrodt AW, Dobrogowska DH. Molecular anatomy of interendothelial junctions in human blood-brain barrier microvessels. Folia Histochem Cytobiol. 2004;42(2):67-75.
Vorbrodt, A. W., & Dobrogowska, D. H. (2004). Molecular anatomy of interendothelial junctions in human blood-brain barrier microvessels. Folia Histochemica Et Cytobiologica, 42(2), 67-75.
Vorbrodt AW, Dobrogowska DH. Molecular Anatomy of Interendothelial Junctions in Human Blood-brain Barrier Microvessels. Folia Histochem Cytobiol. 2004;42(2):67-75. PubMed PMID: 15253128.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular anatomy of interendothelial junctions in human blood-brain barrier microvessels. AU - Vorbrodt,Andrzej W, AU - Dobrogowska,Danuta H, PY - 2004/7/16/pubmed PY - 2005/1/20/medline PY - 2004/7/16/entrez SP - 67 EP - 75 JF - Folia histochemica et cytobiologica JO - Folia Histochem. Cytobiol. VL - 42 IS - 2 N2 - Immunogold cytochemical procedure was used to study the localization at the ultrastructural level of interendothelial junction-associated protein molecules in the human brain blood microvessels, representing the anatomic site of the blood-brain barrier (BBB). Ultrathin sections of Lowicryl K4M-embedded biopsy specimens of human cerebral cortex obtained during surgical procedures were exposed to specific antibodies, followed by colloidal gold-labeled secondary antibodies. All tight junction-specific integral membrane (transmembrane) proteins--occludin, junctional adhesion molecule (JAM-1), and claudin-5--as well as peripheral zonula occludens protein (ZO-1) were highly expressed. Immunoreactivity of the adherens junction-specific transmembrane protein VE-cadherin was of almost similar intensity. Immunolabeling of the adherens junction-associated peripheral proteins--alpha-catenin, beta-catenin, and p120 catenin--although positive, was evidently less intense. The expression of gamma-catenin (plakoglobin) was considered questionable because solitary immunosignals (gold particles) appeared in only a few microvascular profiles. Double labeling of some sections made possible to observe strict colocalization of the junctional molecules, such as occludin and ZO-1 or JAM-1 and VE-cadherin, in the interendothelial junctions. We found that in human brain microvessels, the interendothelial junctional complexes contain molecular components specific for both tight and adherens junctions. It is assumed that the data obtained can help us find the immunodetectable junctional molecules that can serve as sensitive markers of normal or abnormal function of the BBB. SN - 0239-8508 UR - https://www.unboundmedicine.com/medline/citation/15253128/Molecular_anatomy_of_interendothelial_junctions_in_human_blood_brain_barrier_microvessels_ L2 - http://czasopisma.viamedica.pl/fhc/article/view/4660 DB - PRIME DP - Unbound Medicine ER -