Tags

Type your tag names separated by a space and hit enter

Development of pancreatic islets in pancreatic polypeptide-overexpressing mice.
Int J Mol Med. 2004 Aug; 14(2):201-5.IJ

Abstract

Recently we produced pancreatic polypeptide transgenic (PPTG) mice and found that PP was overexpressed in pancreatic islets. The present study examines development of four islet hormones in PPTG mice at embryonic days (ED) 15, 17, and 19, and in adult animals. Adult PPTG mice showed massive aggregation of PP-positive cells and glucagon-positive cells seen at the central area of the islets. Confocal laser microscopic study showed that three islet hormones (insulin, glucagon and PP) were completely overlapped in islets of PPTG mice. Overlapping of somatostatin/glucagon and somatostatin/PP were also increased at the peripheral area of the islets in adult PPTG mice compared to wild-type mice. In prenatal development of pancreatic islets of PPTG mice, somatostatin/glucagon overlapping cells appeared at ED 15, two days earlier than in wild-type mice. Differentiation of these somatostatin/glucagon double-positive cells into single-positive cells was disturbed in the PPTG mice during perinatal to postnatal periods. Differentiation of glucagon/insulin-double positive cells into single-positive cells was disturbed remarkably in postnatal development of the islets of PPTG mice. The present results suggest that early and overexpression of PP may engender the early appearance of somatostatin producing cells; however, that may disturb differentiation of multihormonal immature endocrine cells into single hormonal mature endocrine cells.

Authors+Show Affiliations

Division of Diabetes, Digestive and Kidney Diseases, Department of Clinical Molecular Medicine, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15254766

Citation

Yuzuriha, Hideki, et al. "Development of Pancreatic Islets in Pancreatic Polypeptide-overexpressing Mice." International Journal of Molecular Medicine, vol. 14, no. 2, 2004, pp. 201-5.
Yuzuriha H, Inui A, Asakawa A, et al. Development of pancreatic islets in pancreatic polypeptide-overexpressing mice. Int J Mol Med. 2004;14(2):201-5.
Yuzuriha, H., Inui, A., Asakawa, A., Ueno, N., Sakamaki, R., Shinfuku, N., Kasuga, M., & Fujimiya, M. (2004). Development of pancreatic islets in pancreatic polypeptide-overexpressing mice. International Journal of Molecular Medicine, 14(2), 201-5.
Yuzuriha H, et al. Development of Pancreatic Islets in Pancreatic Polypeptide-overexpressing Mice. Int J Mol Med. 2004;14(2):201-5. PubMed PMID: 15254766.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of pancreatic islets in pancreatic polypeptide-overexpressing mice. AU - Yuzuriha,Hideki, AU - Inui,Akio, AU - Asakawa,Akihiro, AU - Ueno,Naohiko, AU - Sakamaki,Ruka, AU - Shinfuku,Naotaka, AU - Kasuga,Masato, AU - Fujimiya,Mineko, PY - 2004/7/16/pubmed PY - 2005/3/5/medline PY - 2004/7/16/entrez SP - 201 EP - 5 JF - International journal of molecular medicine JO - Int J Mol Med VL - 14 IS - 2 N2 - Recently we produced pancreatic polypeptide transgenic (PPTG) mice and found that PP was overexpressed in pancreatic islets. The present study examines development of four islet hormones in PPTG mice at embryonic days (ED) 15, 17, and 19, and in adult animals. Adult PPTG mice showed massive aggregation of PP-positive cells and glucagon-positive cells seen at the central area of the islets. Confocal laser microscopic study showed that three islet hormones (insulin, glucagon and PP) were completely overlapped in islets of PPTG mice. Overlapping of somatostatin/glucagon and somatostatin/PP were also increased at the peripheral area of the islets in adult PPTG mice compared to wild-type mice. In prenatal development of pancreatic islets of PPTG mice, somatostatin/glucagon overlapping cells appeared at ED 15, two days earlier than in wild-type mice. Differentiation of these somatostatin/glucagon double-positive cells into single-positive cells was disturbed in the PPTG mice during perinatal to postnatal periods. Differentiation of glucagon/insulin-double positive cells into single-positive cells was disturbed remarkably in postnatal development of the islets of PPTG mice. The present results suggest that early and overexpression of PP may engender the early appearance of somatostatin producing cells; however, that may disturb differentiation of multihormonal immature endocrine cells into single hormonal mature endocrine cells. SN - 1107-3756 UR - https://www.unboundmedicine.com/medline/citation/15254766/Development_of_pancreatic_islets_in_pancreatic_polypeptide_overexpressing_mice_ L2 - http://www.spandidos-publications.com/ijmm/14/2/201 DB - PRIME DP - Unbound Medicine ER -