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Substance P (NK1) and somatostatin (sst2A) receptor immunoreactivity in NTS-projecting rat dorsal horn neurones activated by nociceptive afferent input.
J Chem Neuroanat. 2004 Jul; 27(4):251-66.JC

Abstract

Spinal neurones that receive inputs from primary afferent fibres and have axons projecting supraspinally to the medulla oblongata may represent a pathway through which nociceptive and non-nociceptive peripheral stimuli are able to modulate cardiorespiratory reflexes. Expression of the neurokinin-1 (NK1) receptor is believed to be an indicator of lamina I cells that receive nociceptive inputs from substance P releasing afferents, and similarly, sst2A receptor expression may be a marker for neurones receiving somatostatinergic inputs. In this study, immunoreactivity for these two receptors was investigated in rat spinal neurones retrogradely labelled by injections of cholera toxin B or Fluorogold into the nucleus of the solitary tract (NTS). In addition, nociceptive activation of these labelled cells was studied by immunodetection of Fos protein in response to cutaneous and visceral noxious chemical stimuli. NK1 and sst2A receptors in lamina I were localised to mainly separate populations of retrogradely labelled cells with fusiform, flattened and pyramidal morphologies. Examples of projection neurones expressing both receptors were, however observed. With visceral stimulation, many retrogradely labelled cells expressing c-fos were immunoreactive for the NK1 receptor, and a smaller population was sst2A positive. In contrast, with cutaneous stimulation, only NK1 positive retrogradely labelled cells showed c-fos expression. These data provide evidence that lamina I neurones receiving noxious cutaneous and visceral stimuli via NK1 receptor activation project to NTS and so may be involved in coordinating nociceptive and cardiorespiratory responses. Moreover, a subpopulation of projection neurones that respond to visceral stimuli may receive somatostatinergic inputs of peripheral, local or supraspinal origins.

Authors+Show Affiliations

Institute for Cardiovascular Research, School of Medicine, Worsley Building, University of Leeds, Leeds LS2 9JT, UK.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15261332

Citation

Gamboa-Esteves, Filomena O., et al. "Substance P (NK1) and Somatostatin (sst2A) Receptor Immunoreactivity in NTS-projecting Rat Dorsal Horn Neurones Activated By Nociceptive Afferent Input." Journal of Chemical Neuroanatomy, vol. 27, no. 4, 2004, pp. 251-66.
Gamboa-Esteves FO, McWilliam PN, Batten TF. Substance P (NK1) and somatostatin (sst2A) receptor immunoreactivity in NTS-projecting rat dorsal horn neurones activated by nociceptive afferent input. J Chem Neuroanat. 2004;27(4):251-66.
Gamboa-Esteves, F. O., McWilliam, P. N., & Batten, T. F. (2004). Substance P (NK1) and somatostatin (sst2A) receptor immunoreactivity in NTS-projecting rat dorsal horn neurones activated by nociceptive afferent input. Journal of Chemical Neuroanatomy, 27(4), 251-66.
Gamboa-Esteves FO, McWilliam PN, Batten TF. Substance P (NK1) and Somatostatin (sst2A) Receptor Immunoreactivity in NTS-projecting Rat Dorsal Horn Neurones Activated By Nociceptive Afferent Input. J Chem Neuroanat. 2004;27(4):251-66. PubMed PMID: 15261332.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Substance P (NK1) and somatostatin (sst2A) receptor immunoreactivity in NTS-projecting rat dorsal horn neurones activated by nociceptive afferent input. AU - Gamboa-Esteves,Filomena O, AU - McWilliam,Peter N, AU - Batten,Trevor F C, PY - 2003/10/07/received PY - 2004/02/17/revised PY - 2004/04/06/accepted PY - 2004/7/21/pubmed PY - 2004/10/19/medline PY - 2004/7/21/entrez SP - 251 EP - 66 JF - Journal of chemical neuroanatomy JO - J. Chem. Neuroanat. VL - 27 IS - 4 N2 - Spinal neurones that receive inputs from primary afferent fibres and have axons projecting supraspinally to the medulla oblongata may represent a pathway through which nociceptive and non-nociceptive peripheral stimuli are able to modulate cardiorespiratory reflexes. Expression of the neurokinin-1 (NK1) receptor is believed to be an indicator of lamina I cells that receive nociceptive inputs from substance P releasing afferents, and similarly, sst2A receptor expression may be a marker for neurones receiving somatostatinergic inputs. In this study, immunoreactivity for these two receptors was investigated in rat spinal neurones retrogradely labelled by injections of cholera toxin B or Fluorogold into the nucleus of the solitary tract (NTS). In addition, nociceptive activation of these labelled cells was studied by immunodetection of Fos protein in response to cutaneous and visceral noxious chemical stimuli. NK1 and sst2A receptors in lamina I were localised to mainly separate populations of retrogradely labelled cells with fusiform, flattened and pyramidal morphologies. Examples of projection neurones expressing both receptors were, however observed. With visceral stimulation, many retrogradely labelled cells expressing c-fos were immunoreactive for the NK1 receptor, and a smaller population was sst2A positive. In contrast, with cutaneous stimulation, only NK1 positive retrogradely labelled cells showed c-fos expression. These data provide evidence that lamina I neurones receiving noxious cutaneous and visceral stimuli via NK1 receptor activation project to NTS and so may be involved in coordinating nociceptive and cardiorespiratory responses. Moreover, a subpopulation of projection neurones that respond to visceral stimuli may receive somatostatinergic inputs of peripheral, local or supraspinal origins. SN - 0891-0618 UR - https://www.unboundmedicine.com/medline/citation/15261332/Substance_P__NK1__and_somatostatin__sst2A__receptor_immunoreactivity_in_NTS_projecting_rat_dorsal_horn_neurones_activated_by_nociceptive_afferent_input_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S089106180400047X DB - PRIME DP - Unbound Medicine ER -