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Age-related changes of antioxidant enzyme activities, glutathione status and lipid peroxidation in rat erythrocytes after heat stress.
Life Sci. 2004 Aug 13; 75(13):1551-65.LS

Abstract

The aim of this study was to determine whether exposure to heat stress would lead to oxidative stress and whether this effect varied with different exposure periods. We kept 1-, 6- and 12-month-old male Wistar rats at an ambient temperature of either 22 degrees C or 40 degrees C for 3 and 7 days and measured glucose-6-phosphate dehydrogenase (G-6-PD), Cu,Zn-superoxide dismutase (Cu,Zn-SOD), catalase (CAT), selenium-dependent glutathione peroxidase (Se-GSH-Px) and glutathione-S-transferase (GST) activities and levels of thiobarbituric acid-reactive substances (TBARS), reduced glutathione (GSH) and oxidized glutathione (GSSG) in erythrocytes and determined GSH/GSSG ratio, total glutathione and the redox index. G-6-PD and CAT activities were found to be significantly increased in 1- and 6-month-old rats after 3 and 7 days of heat stress, but G-6-PD activities decreased in 12-month-old rats. Cu, Zn-SOD activity decreased in 1-month-old rats after heat stress, whereas it increased in 6- and 12-month-old rats. GST activity increased in all groups. GSH and total GSH levels and GSH/GSSG ratios decreased in 1- and 6-month-old rats but they increased in 12-month-old rats after heat stress. GSSG levels increased in 1- and 6-month-old rats but decreased in 12-month-old rats after heat stress. TBARS levels increased in all groups. Seven days of stress is more effective in altering enzyme activities and levels of GSH, GSSG and TBARS. When the effects of both heat stress and aging were examined together, it was interesting to note that they mostly influenced G-6-PD activity.

Authors+Show Affiliations

Department of Biochemistry, Faculty of Medicine, Akdeniz University, 07070 Antalya, Turkey.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15261761

Citation

Oztürk, Oğuz, and Saadet Gümüşlü. "Age-related Changes of Antioxidant Enzyme Activities, Glutathione Status and Lipid Peroxidation in Rat Erythrocytes After Heat Stress." Life Sciences, vol. 75, no. 13, 2004, pp. 1551-65.
Oztürk O, Gümüşlü S. Age-related changes of antioxidant enzyme activities, glutathione status and lipid peroxidation in rat erythrocytes after heat stress. Life Sci. 2004;75(13):1551-65.
Oztürk, O., & Gümüşlü, S. (2004). Age-related changes of antioxidant enzyme activities, glutathione status and lipid peroxidation in rat erythrocytes after heat stress. Life Sciences, 75(13), 1551-65.
Oztürk O, Gümüşlü S. Age-related Changes of Antioxidant Enzyme Activities, Glutathione Status and Lipid Peroxidation in Rat Erythrocytes After Heat Stress. Life Sci. 2004 Aug 13;75(13):1551-65. PubMed PMID: 15261761.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Age-related changes of antioxidant enzyme activities, glutathione status and lipid peroxidation in rat erythrocytes after heat stress. AU - Oztürk,Oğuz, AU - Gümüşlü,Saadet, PY - 2003/08/15/received PY - 2004/03/04/accepted PY - 2004/7/21/pubmed PY - 2004/8/26/medline PY - 2004/7/21/entrez SP - 1551 EP - 65 JF - Life sciences JO - Life Sci VL - 75 IS - 13 N2 - The aim of this study was to determine whether exposure to heat stress would lead to oxidative stress and whether this effect varied with different exposure periods. We kept 1-, 6- and 12-month-old male Wistar rats at an ambient temperature of either 22 degrees C or 40 degrees C for 3 and 7 days and measured glucose-6-phosphate dehydrogenase (G-6-PD), Cu,Zn-superoxide dismutase (Cu,Zn-SOD), catalase (CAT), selenium-dependent glutathione peroxidase (Se-GSH-Px) and glutathione-S-transferase (GST) activities and levels of thiobarbituric acid-reactive substances (TBARS), reduced glutathione (GSH) and oxidized glutathione (GSSG) in erythrocytes and determined GSH/GSSG ratio, total glutathione and the redox index. G-6-PD and CAT activities were found to be significantly increased in 1- and 6-month-old rats after 3 and 7 days of heat stress, but G-6-PD activities decreased in 12-month-old rats. Cu, Zn-SOD activity decreased in 1-month-old rats after heat stress, whereas it increased in 6- and 12-month-old rats. GST activity increased in all groups. GSH and total GSH levels and GSH/GSSG ratios decreased in 1- and 6-month-old rats but they increased in 12-month-old rats after heat stress. GSSG levels increased in 1- and 6-month-old rats but decreased in 12-month-old rats after heat stress. TBARS levels increased in all groups. Seven days of stress is more effective in altering enzyme activities and levels of GSH, GSSG and TBARS. When the effects of both heat stress and aging were examined together, it was interesting to note that they mostly influenced G-6-PD activity. SN - 0024-3205 UR - https://www.unboundmedicine.com/medline/citation/15261761/Age_related_changes_of_antioxidant_enzyme_activities_glutathione_status_and_lipid_peroxidation_in_rat_erythrocytes_after_heat_stress_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024320504004643 DB - PRIME DP - Unbound Medicine ER -