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Glutamine kinetics and protein turnover in end-stage renal disease.
Am J Physiol Endocrinol Metab. 2005 Jan; 288(1):E37-46.AJ

Abstract

Alanine and glutamine constitute the two most important nitrogen carriers released from the muscle. We studied the intracellular amino acid transport kinetics and protein turnover in nine end-stage renal disease (ESRD) patients and eight controls by use of stable isotopes of phenylalanine, alanine, and glutamine. The amino acid transport kinetics and protein turnover were calculated with a three-pool model from the amino acid concentrations and enrichment in the artery, vein, and muscle compartments. Muscle protein breakdown was more than synthesis (nmol.min(-1).100 ml leg(-1)) during hemodialysis (HD) (169.8 +/- 20.0 vs. 125.9 +/- 21.8, P < 0.05) and in controls (126.9 +/- 6.9 vs. 98.4 +/- 7.5, P < 0.05), but synthesis and catabolism were comparable pre-HD (100.7 +/- 15.7 vs. 103.4 +/- 14.8). Whole body protein catabolism decreased by 15% during HD. The intracellular appearance of alanine (399.0 +/- 47.1 vs. 243.0 +/- 34.689) and glutamine (369.7 +/- 40.6 vs. 235.6 +/- 27.5) from muscle protein breakdown increased during dialysis (nmol.min(-1).100 ml leg(-1), P < 0.01). However, the de novo synthesis of alanine (3,468.9 +/- 572.2 vs. 3,140.5 +/- 467.7) and glutamine (1,751.4 +/- 82.6 vs. 1,782.2 +/- 86.4) did not change significantly intradialysis (nmol.min(-1).100 ml leg(-1)). Branched-chain amino acid catabolism (191.8 +/- 63.4 vs. -59.1 +/- 42.9) and nonprotein glutamate disposal (347.0 +/- 46.3 vs. 222.3 +/- 43.6) increased intradialysis compared with pre-HD (nmol.min(-1).100 ml leg(-1), P < 0.01). The mRNA levels of glutamine synthase (1.45 +/- 0.14 vs. 0.33 +/- 0.08, P < 0.001) and branched-chain keto acid dehydrogenase-E2 (3.86 +/- 0.48 vs. 2.14 +/- 0.27, P < 0.05) in the muscle increased during HD. Thus intracellular concentrations of alanine and glutamine are maintained during HD by augmented release of the amino acids from muscle protein catabolism. Although muscle protein breakdown increased intradialysis, the whole body protein catabolism decreased, suggesting central utilization of amino acids released from skeletal muscle.

Authors+Show Affiliations

Raj DS
Division of Nephrology, University of New Mexico Health Sciences Center, Albuquerque, NM 87131-5271, USA. draj@salud.unm.edu
Welbourne T
No affiliation info available
Dominic EA
No affiliation info available
Waters D
No affiliation info available
Wolfe R
No affiliation info available
Ferrando A
No affiliation info available

MeSH

AdultAlanineArteriesDiabetes Mellitus, Type 2FemaleGlutamic AcidHumansKidney Failure, ChronicMaleMiddle AgedModels, BiologicalMuscle, SkeletalPhenylalanineProteinsRenal DialysisVeins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15265763

Citation

Raj, Dominic S C., et al. "Glutamine Kinetics and Protein Turnover in End-stage Renal Disease." American Journal of Physiology. Endocrinology and Metabolism, vol. 288, no. 1, 2005, pp. E37-46.
Raj DS, Welbourne T, Dominic EA, et al. Glutamine kinetics and protein turnover in end-stage renal disease. Am J Physiol Endocrinol Metab. 2005;288(1):E37-46.
Raj, D. S., Welbourne, T., Dominic, E. A., Waters, D., Wolfe, R., & Ferrando, A. (2005). Glutamine kinetics and protein turnover in end-stage renal disease. American Journal of Physiology. Endocrinology and Metabolism, 288(1), E37-46.
Raj DS, et al. Glutamine Kinetics and Protein Turnover in End-stage Renal Disease. Am J Physiol Endocrinol Metab. 2005;288(1):E37-46. PubMed PMID: 15265763.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glutamine kinetics and protein turnover in end-stage renal disease. AU - Raj,Dominic S C, AU - Welbourne,Tomas, AU - Dominic,Elizabeth A, AU - Waters,Debra, AU - Wolfe,Robert, AU - Ferrando,Arny, Y1 - 2004/07/20/ PY - 2004/7/22/pubmed PY - 2005/3/3/medline PY - 2004/7/22/entrez SP - E37 EP - 46 JF - American journal of physiology. Endocrinology and metabolism JO - Am J Physiol Endocrinol Metab VL - 288 IS - 1 N2 - Alanine and glutamine constitute the two most important nitrogen carriers released from the muscle. We studied the intracellular amino acid transport kinetics and protein turnover in nine end-stage renal disease (ESRD) patients and eight controls by use of stable isotopes of phenylalanine, alanine, and glutamine. The amino acid transport kinetics and protein turnover were calculated with a three-pool model from the amino acid concentrations and enrichment in the artery, vein, and muscle compartments. Muscle protein breakdown was more than synthesis (nmol.min(-1).100 ml leg(-1)) during hemodialysis (HD) (169.8 +/- 20.0 vs. 125.9 +/- 21.8, P < 0.05) and in controls (126.9 +/- 6.9 vs. 98.4 +/- 7.5, P < 0.05), but synthesis and catabolism were comparable pre-HD (100.7 +/- 15.7 vs. 103.4 +/- 14.8). Whole body protein catabolism decreased by 15% during HD. The intracellular appearance of alanine (399.0 +/- 47.1 vs. 243.0 +/- 34.689) and glutamine (369.7 +/- 40.6 vs. 235.6 +/- 27.5) from muscle protein breakdown increased during dialysis (nmol.min(-1).100 ml leg(-1), P < 0.01). However, the de novo synthesis of alanine (3,468.9 +/- 572.2 vs. 3,140.5 +/- 467.7) and glutamine (1,751.4 +/- 82.6 vs. 1,782.2 +/- 86.4) did not change significantly intradialysis (nmol.min(-1).100 ml leg(-1)). Branched-chain amino acid catabolism (191.8 +/- 63.4 vs. -59.1 +/- 42.9) and nonprotein glutamate disposal (347.0 +/- 46.3 vs. 222.3 +/- 43.6) increased intradialysis compared with pre-HD (nmol.min(-1).100 ml leg(-1), P < 0.01). The mRNA levels of glutamine synthase (1.45 +/- 0.14 vs. 0.33 +/- 0.08, P < 0.001) and branched-chain keto acid dehydrogenase-E2 (3.86 +/- 0.48 vs. 2.14 +/- 0.27, P < 0.05) in the muscle increased during HD. Thus intracellular concentrations of alanine and glutamine are maintained during HD by augmented release of the amino acids from muscle protein catabolism. Although muscle protein breakdown increased intradialysis, the whole body protein catabolism decreased, suggesting central utilization of amino acids released from skeletal muscle. SN - 0193-1849 UR - https://www.unboundmedicine.com/medline/citation/15265763/Glutamine_kinetics_and_protein_turnover_in_end_stage_renal_disease_ DB - PRIME DP - Unbound Medicine ER -