Tags

Type your tag names separated by a space and hit enter

Time course of nitric oxide production after endotoxin challenge in mice.
Am J Physiol Endocrinol Metab 2004; 287(5):E912-8AJ

Abstract

Nitric oxide (NO) regulates numerous processes during endotoxemia and inflammation. However, the sequential changes in whole body (Wb) nitric oxide (NO) production during endotoxemia in vivo remain to be clarified. Male Swiss mice were injected intraperitoneally with saline (control group) or lipopolysaccharide (LPS group). After 0, 2, 4, 6, 9, 12, and 24 h, animals received a primed constant infusion of L-[guanidino-(15)N(2)-(2)H(2)]arginine, L-[ureido-(15)N]citrulline, L-[5-(15)N]glutamine, and L-[ring-(2)H(5)]phenylalanine in the jugular vein. Arterial blood was collected for plasma arginine (Arg), citrulline (Cit), glutamine (Gln), and phenylalanine (Phe) concentrations and tracer-to-tracee ratios. NO production was calculated as plasma Arg-to-Cit flux, Wb de novo Arg synthesis as plasma Cit-to-Arg flux, and Wb protein breakdown as plasma Phe flux. LPS reduced plasma Arg and Cit and increased Gln and Phe concentrations. Two peaks of NO production were observed at 4 and 12 h after LPS. Although LPS did not affect total Arg production, de novo Arg production decreased after 12 h. The second peak of NO production coincided with increased Wb Cit, Gln, and Phe production. In conclusion, the curve of NO production in both early and late phases of endotoxemia is not related to plasma Arg kinetics. However, because Wb Cit, Gln, and Phe fluxes increased concomitantly with the second peak of NO production, NO production is probably related to the catabolic phase of endotoxemia.

Authors+Show Affiliations

Division of Nutrition and Metabolism, University Hospital Clementino Fraga Filho, Federal University of Rio de Janeiro, 21941-590 Rio de Janeiro, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15265764

Citation

Braulio, Valeria B., et al. "Time Course of Nitric Oxide Production After Endotoxin Challenge in Mice." American Journal of Physiology. Endocrinology and Metabolism, vol. 287, no. 5, 2004, pp. E912-8.
Braulio VB, Ten Have GA, Vissers YL, et al. Time course of nitric oxide production after endotoxin challenge in mice. Am J Physiol Endocrinol Metab. 2004;287(5):E912-8.
Braulio, V. B., Ten Have, G. A., Vissers, Y. L., & Deutz, N. E. (2004). Time course of nitric oxide production after endotoxin challenge in mice. American Journal of Physiology. Endocrinology and Metabolism, 287(5), pp. E912-8.
Braulio VB, et al. Time Course of Nitric Oxide Production After Endotoxin Challenge in Mice. Am J Physiol Endocrinol Metab. 2004;287(5):E912-8. PubMed PMID: 15265764.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Time course of nitric oxide production after endotoxin challenge in mice. AU - Braulio,Valeria B, AU - Ten Have,Gabrie A M, AU - Vissers,Yvonne L J, AU - Deutz,Nicolaas E P, Y1 - 2004/07/20/ PY - 2004/7/22/pubmed PY - 2004/12/16/medline PY - 2004/7/22/entrez SP - E912 EP - 8 JF - American journal of physiology. Endocrinology and metabolism JO - Am. J. Physiol. Endocrinol. Metab. VL - 287 IS - 5 N2 - Nitric oxide (NO) regulates numerous processes during endotoxemia and inflammation. However, the sequential changes in whole body (Wb) nitric oxide (NO) production during endotoxemia in vivo remain to be clarified. Male Swiss mice were injected intraperitoneally with saline (control group) or lipopolysaccharide (LPS group). After 0, 2, 4, 6, 9, 12, and 24 h, animals received a primed constant infusion of L-[guanidino-(15)N(2)-(2)H(2)]arginine, L-[ureido-(15)N]citrulline, L-[5-(15)N]glutamine, and L-[ring-(2)H(5)]phenylalanine in the jugular vein. Arterial blood was collected for plasma arginine (Arg), citrulline (Cit), glutamine (Gln), and phenylalanine (Phe) concentrations and tracer-to-tracee ratios. NO production was calculated as plasma Arg-to-Cit flux, Wb de novo Arg synthesis as plasma Cit-to-Arg flux, and Wb protein breakdown as plasma Phe flux. LPS reduced plasma Arg and Cit and increased Gln and Phe concentrations. Two peaks of NO production were observed at 4 and 12 h after LPS. Although LPS did not affect total Arg production, de novo Arg production decreased after 12 h. The second peak of NO production coincided with increased Wb Cit, Gln, and Phe production. In conclusion, the curve of NO production in both early and late phases of endotoxemia is not related to plasma Arg kinetics. However, because Wb Cit, Gln, and Phe fluxes increased concomitantly with the second peak of NO production, NO production is probably related to the catabolic phase of endotoxemia. SN - 0193-1849 UR - https://www.unboundmedicine.com/medline/citation/15265764/Time_course_of_nitric_oxide_production_after_endotoxin_challenge_in_mice_ L2 - http://www.physiology.org/doi/full/10.1152/ajpendo.00540.2003?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -