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Different cytokine profiles in patients with chronic and acute reactive arthritis.
Rheumatology (Oxford). 2004 Oct; 43(10):1300-4.R

Abstract

OBJECTIVE

Analysis of cytokine production in patients with acute and chronic reactive arthritis (AcReA/ChrReA) in order to search for new treatment possibilities.

METHODS

Cytokine production by peripheral blood and synovial fluid mononuclear cells (PBMCs/SFMCs) of 28 patients with AcReA, 27 patients with ChrReA, 26 patients with rheumatoid arthritis (RA) and 31 healthy controls was analysed by enzyme-linked immunosorbent assay (ELISA) and flow-cytometry. Production of tumour necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma) and interleukin (IL)-10 was measured by ELISA, while the percentages of TNF-alpha-, IFN-gamma- and IL-4-positive CD3+ cells were determined in the same groups of patients and healthy subjects using flow cytometry.

RESULTS

Spontaneous TNF-alpha production observed in PBMCs of ChrReA, but not of AcReA, patients was significantly higher (P<0.001) than in healthy controls. The percentages of TNF-alpha-positive CD3+ blood cells in ChrReA exceeded that of RA patients and healthy controls (P<0.05 and P<0.001, respectively). Also, the percentages of IFN-gamma-positive CD3+ cells were significantly higher in peripheral blood and synovial fluid of ChrReA patients (P<0.05 and P<0.05, respectively) as compared with AcReA. In ChrReA spontaneous IL-10 production in PBMCs was similar to that observed in healthy controls, while in RA and AcReA the production of IL-10 was significantly increased (P<0.05 and P<0.05, respectively). IL-4 production was low in all study groups with no significant differences detected.

CONCLUSIONS

High production of TNF-alpha and IFN-gamma detected in ChrReA supports the possible use of anti-TNF-alpha treatment in ChrReA.

Authors+Show Affiliations

Department of Rheumatology, Vilnius University Institute of Experimental and Clinical Medicine, Zygimantu 9, LT-2600, Vilnius, Lithuania. irena.butrimiene@santa.ltNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15266062

Citation

Butrimiene, I, et al. "Different Cytokine Profiles in Patients With Chronic and Acute Reactive Arthritis." Rheumatology (Oxford, England), vol. 43, no. 10, 2004, pp. 1300-4.
Butrimiene I, Jarmalaite S, Ranceva J, et al. Different cytokine profiles in patients with chronic and acute reactive arthritis. Rheumatology (Oxford). 2004;43(10):1300-4.
Butrimiene, I., Jarmalaite, S., Ranceva, J., Venalis, A., Jasiuleviciute, L., & Zvirbliene, A. (2004). Different cytokine profiles in patients with chronic and acute reactive arthritis. Rheumatology (Oxford, England), 43(10), 1300-4.
Butrimiene I, et al. Different Cytokine Profiles in Patients With Chronic and Acute Reactive Arthritis. Rheumatology (Oxford). 2004;43(10):1300-4. PubMed PMID: 15266062.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Different cytokine profiles in patients with chronic and acute reactive arthritis. AU - Butrimiene,I, AU - Jarmalaite,S, AU - Ranceva,J, AU - Venalis,A, AU - Jasiuleviciute,L, AU - Zvirbliene,A, Y1 - 2004/07/20/ PY - 2004/7/22/pubmed PY - 2004/11/2/medline PY - 2004/7/22/entrez SP - 1300 EP - 4 JF - Rheumatology (Oxford, England) JO - Rheumatology (Oxford) VL - 43 IS - 10 N2 - OBJECTIVE: Analysis of cytokine production in patients with acute and chronic reactive arthritis (AcReA/ChrReA) in order to search for new treatment possibilities. METHODS: Cytokine production by peripheral blood and synovial fluid mononuclear cells (PBMCs/SFMCs) of 28 patients with AcReA, 27 patients with ChrReA, 26 patients with rheumatoid arthritis (RA) and 31 healthy controls was analysed by enzyme-linked immunosorbent assay (ELISA) and flow-cytometry. Production of tumour necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma) and interleukin (IL)-10 was measured by ELISA, while the percentages of TNF-alpha-, IFN-gamma- and IL-4-positive CD3+ cells were determined in the same groups of patients and healthy subjects using flow cytometry. RESULTS: Spontaneous TNF-alpha production observed in PBMCs of ChrReA, but not of AcReA, patients was significantly higher (P<0.001) than in healthy controls. The percentages of TNF-alpha-positive CD3+ blood cells in ChrReA exceeded that of RA patients and healthy controls (P<0.05 and P<0.001, respectively). Also, the percentages of IFN-gamma-positive CD3+ cells were significantly higher in peripheral blood and synovial fluid of ChrReA patients (P<0.05 and P<0.05, respectively) as compared with AcReA. In ChrReA spontaneous IL-10 production in PBMCs was similar to that observed in healthy controls, while in RA and AcReA the production of IL-10 was significantly increased (P<0.05 and P<0.05, respectively). IL-4 production was low in all study groups with no significant differences detected. CONCLUSIONS: High production of TNF-alpha and IFN-gamma detected in ChrReA supports the possible use of anti-TNF-alpha treatment in ChrReA. SN - 1462-0324 UR - https://www.unboundmedicine.com/medline/citation/15266062/Different_cytokine_profiles_in_patients_with_chronic_and_acute_reactive_arthritis_ L2 - https://academic.oup.com/rheumatology/article-lookup/doi/10.1093/rheumatology/keh323 DB - PRIME DP - Unbound Medicine ER -