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[Food effect on the physiological condition of the mussel Perna viridis (Bivalvia: Mytilidae), using the RNA/DNA ratio].
Rev Biol Trop. 2000 Dec; 48 Suppl 1:171-82.RB

Abstract

The green mussel, Perna viridis, became widespread in the northern coast of Sucre State since its arrival to Venezuela in 1993. RNA/DNA and Protein/DNA ratios were used to study the effect of starvation on its instantaneous growth. The mussels were collected in La Esmeralda and Chacopata, acclimatized in the laboratory for four weeks and maintained for another six weeks in two groups: one fed ad libitum and another without food (this later group was later fed for two additional weeks). Protein (colorimetric method), and nucleic acid concentrations (RNA and DNA, fluorometric method with ethidium bromide) were measured in adductor muscle, digestive gland and gills. The instantaneous growth was assessed using RNA/DNA and Protein/DNA rations. These indexes were always higher in the fed organisms. Animals from Chacopata were in better physiological condition that those from La Esmeralda during the abstinence time (six weeks). Muscle was the best tissue to determine instantaneous growth. The RNA/DNA ratio is a reliable index to determine the physiological condition and instantaneous growth of this species.

Authors+Show Affiliations

Departamento de Biología, Escuela Ciencias, Núcleo de Sucre, Universidad de Oriente, Cumaná, Venezuela.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

spa

PubMed ID

15266805

Citation

Bracho, M A., et al. "[Food Effect On the Physiological Condition of the Mussel Perna Viridis (Bivalvia: Mytilidae), Using the RNA/DNA Ratio]." Revista De Biologia Tropical, vol. 48 Suppl 1, 2000, pp. 171-82.
Bracho MA, Segnini de Bravo MI, Viñoles I, et al. [Food effect on the physiological condition of the mussel Perna viridis (Bivalvia: Mytilidae), using the RNA/DNA ratio]. Rev Biol Trop. 2000;48 Suppl 1:171-82.
Bracho, M. A., Segnini de Bravo, M. I., Viñoles, I., & Chung, K. S. (2000). [Food effect on the physiological condition of the mussel Perna viridis (Bivalvia: Mytilidae), using the RNA/DNA ratio]. Revista De Biologia Tropical, 48 Suppl 1, 171-82.
Bracho MA, et al. [Food Effect On the Physiological Condition of the Mussel Perna Viridis (Bivalvia: Mytilidae), Using the RNA/DNA Ratio]. Rev Biol Trop. 2000;48 Suppl 1:171-82. PubMed PMID: 15266805.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Food effect on the physiological condition of the mussel Perna viridis (Bivalvia: Mytilidae), using the RNA/DNA ratio]. AU - Bracho,M A, AU - Segnini de Bravo,M I, AU - Viñoles,I, AU - Chung,K S, PY - 2004/7/23/pubmed PY - 2005/3/11/medline PY - 2004/7/23/entrez SP - 171 EP - 82 JF - Revista de biologia tropical JO - Rev. Biol. Trop. VL - 48 Suppl 1 N2 - The green mussel, Perna viridis, became widespread in the northern coast of Sucre State since its arrival to Venezuela in 1993. RNA/DNA and Protein/DNA ratios were used to study the effect of starvation on its instantaneous growth. The mussels were collected in La Esmeralda and Chacopata, acclimatized in the laboratory for four weeks and maintained for another six weeks in two groups: one fed ad libitum and another without food (this later group was later fed for two additional weeks). Protein (colorimetric method), and nucleic acid concentrations (RNA and DNA, fluorometric method with ethidium bromide) were measured in adductor muscle, digestive gland and gills. The instantaneous growth was assessed using RNA/DNA and Protein/DNA rations. These indexes were always higher in the fed organisms. Animals from Chacopata were in better physiological condition that those from La Esmeralda during the abstinence time (six weeks). Muscle was the best tissue to determine instantaneous growth. The RNA/DNA ratio is a reliable index to determine the physiological condition and instantaneous growth of this species. SN - 0034-7744 UR - https://www.unboundmedicine.com/medline/citation/15266805/[Food_effect_on_the_physiological_condition_of_the_mussel_Perna_viridis__Bivalvia:_Mytilidae__using_the_RNA/DNA_ratio]_ DB - PRIME DP - Unbound Medicine ER -
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