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[Antimicrobial activity of organic extracts isolated from Aplysina fistularis (Demospongiae: Aplysinidae)].
Rev Biol Trop. 2000 Dec; 48 Suppl 1:199-206.RB

Abstract

Organic extracts of the sponge Aplysina fistularis (Pallas 1766) were tested for antimicrobial activity against Gram positive bacteria (Staphylococcus aureus) and Gram negative bacteria (Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa). The minimal inhibitory concentration (MIC) and toxic activity of extract were determined. Susceptibility trials of organic fractions obtained by VLC: Hexane, EtOAc and CHCl3 showed that EtOAc fraction has antibacterial activity against E. coli, while CHCl3 fraction inhibited E. coli and S. aureus growth. The later refractioning of EtOAc fraction and the biodirected assays showed that fractions F12 and F13 of EtOAc/Hex and EtOAc F14 were bioactive against Gram positive and Gram negative bacteria. Only EtOAc/MeOH Sf2 from subfractionig of EtOAc F14 produced inhibition for E. coli and S. aureus. In Sf2 EtOAc/MeOH, MIC was moderate for S. aureus (MIC > 256 g/ml). F4 CHCl3/MeOH produced a high inhibition in S. aureus (MIC = 0.125 g/ml) and for E. coli (MIC > 16 g/ml). F10 CHCl3/MeOH showed a moderate activity against S. aureus (MIC > 128 g/ml) and low activity against E. coli (MIC = 512 g/ml). F10 CHCL3/MeOH did no present toxic activity against Artemia salina. The fractiorts F4 CHCL3/MeOH and Sf2 EtOAc/MeOH were toxic for this organism when the concentration was higher than 100 microg/ml. LC50 in both cases was 548.4 and 243.4 microg/ml respectively. Secondary metabolites of medium polarity obtained from A. fistularis have a wide spectrum of anti bacterial activity. Toxicity analysis suggests that only F10 CHCL3/MeOH has potential as an antimicrobial agent for clinical use.

Authors+Show Affiliations

Ecuela de Medicina, Núcleo de Anzoátegui, Pto. La Cruz, Universidad de Oriente, Cumaná, Venezuela.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

spa

PubMed ID

15266809

Citation

Morales, T, et al. "[Antimicrobial Activity of Organic Extracts Isolated From Aplysina Fistularis (Demospongiae: Aplysinidae)]." Revista De Biologia Tropical, vol. 48 Suppl 1, 2000, pp. 199-206.
Morales T, Cubero J, Lanz Z, et al. [Antimicrobial activity of organic extracts isolated from Aplysina fistularis (Demospongiae: Aplysinidae)]. Rev Biol Trop. 2000;48 Suppl 1:199-206.
Morales, T., Cubero, J., Lanz, Z., Gómez-Guiñán, Y., & Segnini-Bravo, M. I. (2000). [Antimicrobial activity of organic extracts isolated from Aplysina fistularis (Demospongiae: Aplysinidae)]. Revista De Biologia Tropical, 48 Suppl 1, 199-206.
Morales T, et al. [Antimicrobial Activity of Organic Extracts Isolated From Aplysina Fistularis (Demospongiae: Aplysinidae)]. Rev Biol Trop. 2000;48 Suppl 1:199-206. PubMed PMID: 15266809.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Antimicrobial activity of organic extracts isolated from Aplysina fistularis (Demospongiae: Aplysinidae)]. AU - Morales,T, AU - Cubero,J, AU - Lanz,Z, AU - Gómez-Guiñán,Y, AU - Segnini-Bravo,M I, PY - 2004/7/23/pubmed PY - 2005/3/11/medline PY - 2004/7/23/entrez SP - 199 EP - 206 JF - Revista de biologia tropical JO - Rev Biol Trop VL - 48 Suppl 1 N2 - Organic extracts of the sponge Aplysina fistularis (Pallas 1766) were tested for antimicrobial activity against Gram positive bacteria (Staphylococcus aureus) and Gram negative bacteria (Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa). The minimal inhibitory concentration (MIC) and toxic activity of extract were determined. Susceptibility trials of organic fractions obtained by VLC: Hexane, EtOAc and CHCl3 showed that EtOAc fraction has antibacterial activity against E. coli, while CHCl3 fraction inhibited E. coli and S. aureus growth. The later refractioning of EtOAc fraction and the biodirected assays showed that fractions F12 and F13 of EtOAc/Hex and EtOAc F14 were bioactive against Gram positive and Gram negative bacteria. Only EtOAc/MeOH Sf2 from subfractionig of EtOAc F14 produced inhibition for E. coli and S. aureus. In Sf2 EtOAc/MeOH, MIC was moderate for S. aureus (MIC > 256 g/ml). F4 CHCl3/MeOH produced a high inhibition in S. aureus (MIC = 0.125 g/ml) and for E. coli (MIC > 16 g/ml). F10 CHCl3/MeOH showed a moderate activity against S. aureus (MIC > 128 g/ml) and low activity against E. coli (MIC = 512 g/ml). F10 CHCL3/MeOH did no present toxic activity against Artemia salina. The fractiorts F4 CHCL3/MeOH and Sf2 EtOAc/MeOH were toxic for this organism when the concentration was higher than 100 microg/ml. LC50 in both cases was 548.4 and 243.4 microg/ml respectively. Secondary metabolites of medium polarity obtained from A. fistularis have a wide spectrum of anti bacterial activity. Toxicity analysis suggests that only F10 CHCL3/MeOH has potential as an antimicrobial agent for clinical use. SN - 0034-7744 UR - https://www.unboundmedicine.com/medline/citation/15266809/[Antimicrobial_activity_of_organic_extracts_isolated_from_Aplysina_fistularis__Demospongiae:_Aplysinidae_]_ L2 - https://medlineplus.gov/antibiotics.html DB - PRIME DP - Unbound Medicine ER -