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[Effect of BMP-7 on the transdifferentiation of cultured human tubular epithelial cell induced by TGF-beta1].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2004 Jun; 26(3):274-8.ZY

Abstract

OBJECTIVE

To observe the effect of bone morphogenetic protein-7 (BMP-7) on the transdifferentiation of cultured human tubular epithelial cell (HKC) induced by TGF-beta1 and to elucidate its possible mechanism.

METHODS

The cultured HKC cells were divided into 5 groups: serum-free group (negative control); single TGF-beta1 treated group (positive control); single BMP-7 treated group; combined TGF-beta1 and BMP-7 treated group; and BMP-7 pre-treated group. Expression of keratin of HKC cells was assessed by indirect enzyme immunohistochemistry (IEI), expression of alpha-smooth muscle actin (alpha-SMA) and E-cadherin by immunohistological method, percentage of alpha-SMA positive HKC cells by flow cytometry, and mRNA expression of alpha-SMA, TGF-beta1, and TGF-beta type II receptor by reverse transcription PCR.

RESULTS

The expression of alpha-SMA and the percentage of alpha-SMA positive HKC cells markedly increased after having been treated by TGF-beta1 while the expression of E-cadherin and keratin decreased. In the group pre-treated with BMP-7 (50 ng/ml) and then added with TGF-beta1 (8 ng/ml), expression of alpha-SMA was significantly lower than in the positive control group, while expression of E-cadherin and keratin significantly higher than in the positive control group. Measurement of the percentage of alpha-SMA positive HKC found significant deference between the combined TGF-beta1 and BMP-7 treated group and the positive control group (9.7% vs 19.8%; 5.8% vs 19.8%; P < 0.05). Significant difference existed between the BMP-7 (50 ng/ml) pre-treated group and the positive control group (8.7% vs 19.8%, P < 0.05). mRNA expression of alpha-SMA was measured by RT-PCR and the results showed that it significantly decreased in the group treated or pre-treated with BMP-7 (50 ng/ml) (15% and 12% of the results in the positive control group, respectively). The mRNA expression levels of both TGF-beta1 and its type II receptor significantly decreased (28% and 19%; 47% and 36%, compared with the positive control group, respectively).

CONCLUSION

Transdifferentiation of cultured renal epithelial cell induced by TGF-beta1 can be inhibittd by certain levels of BMP-7, cultured together with TGF-beta1 or pretreated. BMP-7 can prevent and inhibit the mRNA expression of TGF-beta1 and its type II receptor, which may be an important mechanism by which BMP-7 inhibit the transdifferentiation of renal tubular epithelial cell.

Authors+Show Affiliations

Tan XY
Department of Nephrology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
Zheng FL
No affiliation info available
Yang JH
No affiliation info available
Duan L
No affiliation info available
Li Y
No affiliation info available
Zhou QG
No affiliation info available

MeSH

ActinsBone Morphogenetic Protein 7Bone Morphogenetic ProteinsCell DifferentiationCells, CulturedEpithelial CellsHumansKidney TubulesPolymerase Chain ReactionRNA, MessengerTransforming Growth Factor betaTransforming Growth Factor beta1

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

15266829

Citation

Tan, Xiao-yue, et al. "[Effect of BMP-7 On the Transdifferentiation of Cultured Human Tubular Epithelial Cell Induced By TGF-beta1]." Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae, vol. 26, no. 3, 2004, pp. 274-8.
Tan XY, Zheng FL, Yang JH, et al. [Effect of BMP-7 on the transdifferentiation of cultured human tubular epithelial cell induced by TGF-beta1]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2004;26(3):274-8.
Tan, X. Y., Zheng, F. L., Yang, J. H., Duan, L., Li, Y., & Zhou, Q. G. (2004). [Effect of BMP-7 on the transdifferentiation of cultured human tubular epithelial cell induced by TGF-beta1]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae, 26(3), 274-8.
Tan XY, et al. [Effect of BMP-7 On the Transdifferentiation of Cultured Human Tubular Epithelial Cell Induced By TGF-beta1]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2004;26(3):274-8. PubMed PMID: 15266829.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of BMP-7 on the transdifferentiation of cultured human tubular epithelial cell induced by TGF-beta1]. AU - Tan,Xiao-yue, AU - Zheng,Fa-lei, AU - Yang,Ji-hong, AU - Duan,Lin, AU - Li,Yan, AU - Zhou,Qiu-gen, PY - 2004/7/23/pubmed PY - 2004/8/25/medline PY - 2004/7/23/entrez SP - 274 EP - 8 JF - Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae JO - Zhongguo Yi Xue Ke Xue Yuan Xue Bao VL - 26 IS - 3 N2 - OBJECTIVE: To observe the effect of bone morphogenetic protein-7 (BMP-7) on the transdifferentiation of cultured human tubular epithelial cell (HKC) induced by TGF-beta1 and to elucidate its possible mechanism. METHODS: The cultured HKC cells were divided into 5 groups: serum-free group (negative control); single TGF-beta1 treated group (positive control); single BMP-7 treated group; combined TGF-beta1 and BMP-7 treated group; and BMP-7 pre-treated group. Expression of keratin of HKC cells was assessed by indirect enzyme immunohistochemistry (IEI), expression of alpha-smooth muscle actin (alpha-SMA) and E-cadherin by immunohistological method, percentage of alpha-SMA positive HKC cells by flow cytometry, and mRNA expression of alpha-SMA, TGF-beta1, and TGF-beta type II receptor by reverse transcription PCR. RESULTS: The expression of alpha-SMA and the percentage of alpha-SMA positive HKC cells markedly increased after having been treated by TGF-beta1 while the expression of E-cadherin and keratin decreased. In the group pre-treated with BMP-7 (50 ng/ml) and then added with TGF-beta1 (8 ng/ml), expression of alpha-SMA was significantly lower than in the positive control group, while expression of E-cadherin and keratin significantly higher than in the positive control group. Measurement of the percentage of alpha-SMA positive HKC found significant deference between the combined TGF-beta1 and BMP-7 treated group and the positive control group (9.7% vs 19.8%; 5.8% vs 19.8%; P < 0.05). Significant difference existed between the BMP-7 (50 ng/ml) pre-treated group and the positive control group (8.7% vs 19.8%, P < 0.05). mRNA expression of alpha-SMA was measured by RT-PCR and the results showed that it significantly decreased in the group treated or pre-treated with BMP-7 (50 ng/ml) (15% and 12% of the results in the positive control group, respectively). The mRNA expression levels of both TGF-beta1 and its type II receptor significantly decreased (28% and 19%; 47% and 36%, compared with the positive control group, respectively). CONCLUSION: Transdifferentiation of cultured renal epithelial cell induced by TGF-beta1 can be inhibittd by certain levels of BMP-7, cultured together with TGF-beta1 or pretreated. BMP-7 can prevent and inhibit the mRNA expression of TGF-beta1 and its type II receptor, which may be an important mechanism by which BMP-7 inhibit the transdifferentiation of renal tubular epithelial cell. SN - 1000-503X UR - https://www.unboundmedicine.com/medline/citation/15266829/[Effect_of_BMP_7_on_the_transdifferentiation_of_cultured_human_tubular_epithelial_cell_induced_by_TGF_beta1]_ DB - PRIME DP - Unbound Medicine ER -
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