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Occurrence of tetracycline resistance genes tet(M) and tet(S) in bacteria from marine aquaculture sites.
FEMS Microbiol Lett. 2004 Aug 01; 237(1):147-56.FM

Abstract

Occurrence of tetracycline resistance genes encoding ribosomal protection proteins was examined in 151 tetracycline-resistant bacterial isolates from fish and seawater at coastal aquaculture sites in Japan and Korea. The tet(M) gene was detected in 34 Japanese and Korean isolates, which included Vibrio sp., Lactococcus garvieae, Photobacterium damsela subsp. piscicida, and unidentified Gram-positive bacteria. The majority of these bacterial isolates displayed high-level resistance with a minimum inhibitory concentrations (MICs) equal to or greater than 250 microg/ml of oxytetracycline and only four isolates had MICs less than 31.3 microg/ml. 16S rDNA RFLP typing of tet(M)-positive Vibrio isolates suggests that these are clonal populations of the same phylotype specific to a particular location. One Vibrio clone (phylotype III), however, is widely disseminated, being detected during different sampling years, at different locations, and in different fish species in both Japan and Korea. The tet(S) gene was detected in L. garvieae from yellowtail in Japan and in Vibrio sp. from seawater in Korea. This is the first report of tet(S) occurrence in Gram-negative facultative anaerobes. These results suggest that tet(M) and tet(S) genes are present in fish intestinal and seawater bacteria at aquaculture sites and could be an important reservoir of tetracycline resistance genes in the marine environment.

Authors+Show Affiliations

Center for Marine Environmental Studies (CMES), Ehime University, Matsuyama 790-8577, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15268950

Citation

Kim, Seok-Ryel, et al. "Occurrence of Tetracycline Resistance Genes tet(M) and tet(S) in Bacteria From Marine Aquaculture Sites." FEMS Microbiology Letters, vol. 237, no. 1, 2004, pp. 147-56.
Kim SR, Nonaka L, Suzuki S. Occurrence of tetracycline resistance genes tet(M) and tet(S) in bacteria from marine aquaculture sites. FEMS Microbiol Lett. 2004;237(1):147-56.
Kim, S. R., Nonaka, L., & Suzuki, S. (2004). Occurrence of tetracycline resistance genes tet(M) and tet(S) in bacteria from marine aquaculture sites. FEMS Microbiology Letters, 237(1), 147-56.
Kim SR, Nonaka L, Suzuki S. Occurrence of Tetracycline Resistance Genes tet(M) and tet(S) in Bacteria From Marine Aquaculture Sites. FEMS Microbiol Lett. 2004 Aug 1;237(1):147-56. PubMed PMID: 15268950.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Occurrence of tetracycline resistance genes tet(M) and tet(S) in bacteria from marine aquaculture sites. AU - Kim,Seok-Ryel, AU - Nonaka,Lisa, AU - Suzuki,Satoru, PY - 2004/03/17/received PY - 2004/04/28/revised PY - 2004/06/16/accepted PY - 2004/7/23/pubmed PY - 2005/2/16/medline PY - 2004/7/23/entrez SP - 147 EP - 56 JF - FEMS microbiology letters JO - FEMS Microbiol Lett VL - 237 IS - 1 N2 - Occurrence of tetracycline resistance genes encoding ribosomal protection proteins was examined in 151 tetracycline-resistant bacterial isolates from fish and seawater at coastal aquaculture sites in Japan and Korea. The tet(M) gene was detected in 34 Japanese and Korean isolates, which included Vibrio sp., Lactococcus garvieae, Photobacterium damsela subsp. piscicida, and unidentified Gram-positive bacteria. The majority of these bacterial isolates displayed high-level resistance with a minimum inhibitory concentrations (MICs) equal to or greater than 250 microg/ml of oxytetracycline and only four isolates had MICs less than 31.3 microg/ml. 16S rDNA RFLP typing of tet(M)-positive Vibrio isolates suggests that these are clonal populations of the same phylotype specific to a particular location. One Vibrio clone (phylotype III), however, is widely disseminated, being detected during different sampling years, at different locations, and in different fish species in both Japan and Korea. The tet(S) gene was detected in L. garvieae from yellowtail in Japan and in Vibrio sp. from seawater in Korea. This is the first report of tet(S) occurrence in Gram-negative facultative anaerobes. These results suggest that tet(M) and tet(S) genes are present in fish intestinal and seawater bacteria at aquaculture sites and could be an important reservoir of tetracycline resistance genes in the marine environment. SN - 0378-1097 UR - https://www.unboundmedicine.com/medline/citation/15268950/Occurrence_of_tetracycline_resistance_genes_tet_M__and_tet_S__in_bacteria_from_marine_aquaculture_sites_ DB - PRIME DP - Unbound Medicine ER -