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Preparation of recombinant proteins in milk.
Methods Mol Biol. 2004; 267:485-94.MM

Abstract

Using transgenic animals as the source of recombinant proteins has several specific advantages. Large amounts of proteins can be obtained, essentially from milk. These proteins are often properly processed. They are in a number of cases correctly folded, assembled, cleaved, glycosylated, gamma-carboxylated, and so on. Purification of recombinant proteins from milk is not a particularly difficult task. The level of expression of foreign genes in milk cannot be predicted in all cases and appropriate vectors must be used. Generation of transgenic mice is popular but their production is quite limited. Transgenesis in larger animals, rabbits and farm animals, is achieved essentially by a few companies. Some recombinant proteins may be found in blood circulation and alter animal health. Milk from transgenic animals has become a quite attractive alternative to other sources of recombinant proteins.

Authors+Show Affiliations

Biologie du Développement et Reproduction, Institute National de la Recherche Agronomique, France.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15269445

Citation

Houdebine, Louis-Marie. "Preparation of Recombinant Proteins in Milk." Methods in Molecular Biology (Clifton, N.J.), vol. 267, 2004, pp. 485-94.
Houdebine LM. Preparation of recombinant proteins in milk. Methods Mol Biol. 2004;267:485-94.
Houdebine, L. M. (2004). Preparation of recombinant proteins in milk. Methods in Molecular Biology (Clifton, N.J.), 267, 485-94.
Houdebine LM. Preparation of Recombinant Proteins in Milk. Methods Mol Biol. 2004;267:485-94. PubMed PMID: 15269445.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation of recombinant proteins in milk. A1 - Houdebine,Louis-Marie, PY - 2004/7/23/pubmed PY - 2004/10/8/medline PY - 2004/7/23/entrez SP - 485 EP - 94 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol. Biol. VL - 267 N2 - Using transgenic animals as the source of recombinant proteins has several specific advantages. Large amounts of proteins can be obtained, essentially from milk. These proteins are often properly processed. They are in a number of cases correctly folded, assembled, cleaved, glycosylated, gamma-carboxylated, and so on. Purification of recombinant proteins from milk is not a particularly difficult task. The level of expression of foreign genes in milk cannot be predicted in all cases and appropriate vectors must be used. Generation of transgenic mice is popular but their production is quite limited. Transgenesis in larger animals, rabbits and farm animals, is achieved essentially by a few companies. Some recombinant proteins may be found in blood circulation and alter animal health. Milk from transgenic animals has become a quite attractive alternative to other sources of recombinant proteins. SN - 1064-3745 UR - https://www.unboundmedicine.com/medline/citation/15269445/Preparation_of_recombinant_proteins_in_milk_ L2 - https://dx.doi.org/10.1385/1-59259-774-2:485 DB - PRIME DP - Unbound Medicine ER -