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Isolation of a clone encoding a second dragline silk fibroin. Nephila clavipes dragline silk is a two-protein fiber.
J Biol Chem 1992; 267(27):19320-4JB

Abstract

Spider dragline silk is a unique protein fiber possessing both high tensile strength and high elasticity. A partial cDNA clone for one dragline silk protein (Spidroin 1) was previously isolated. However, the predicted amino acid sequence could not account for the amino acid composition of dragline silk. We have isolated a partial cDNA clone for another dragline silk protein (Spidroin 2), demonstrating that dragline silk is composed of multiple proteins. The amino acid sequence exhibits an entirely different repetitive motif than Spidroin 1. Spidroin 2 is predicted to consist of linked beta-turns in proline-rich regions which alternate with beta-sheet regions composed of polyalanine segments. This structure for Spidroin 2 provides a model for dragline silk structure and function.

Authors+Show Affiliations

Department of Molecular Biology, University of Wyoming, Laramie 82071.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

1527052

Citation

Hinman, M B., and R V. Lewis. "Isolation of a Clone Encoding a Second Dragline Silk Fibroin. Nephila Clavipes Dragline Silk Is a Two-protein Fiber." The Journal of Biological Chemistry, vol. 267, no. 27, 1992, pp. 19320-4.
Hinman MB, Lewis RV. Isolation of a clone encoding a second dragline silk fibroin. Nephila clavipes dragline silk is a two-protein fiber. J Biol Chem. 1992;267(27):19320-4.
Hinman, M. B., & Lewis, R. V. (1992). Isolation of a clone encoding a second dragline silk fibroin. Nephila clavipes dragline silk is a two-protein fiber. The Journal of Biological Chemistry, 267(27), pp. 19320-4.
Hinman MB, Lewis RV. Isolation of a Clone Encoding a Second Dragline Silk Fibroin. Nephila Clavipes Dragline Silk Is a Two-protein Fiber. J Biol Chem. 1992 Sep 25;267(27):19320-4. PubMed PMID: 1527052.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation of a clone encoding a second dragline silk fibroin. Nephila clavipes dragline silk is a two-protein fiber. AU - Hinman,M B, AU - Lewis,R V, PY - 1992/9/25/pubmed PY - 1992/9/25/medline PY - 1992/9/25/entrez SP - 19320 EP - 4 JF - The Journal of biological chemistry JO - J. Biol. Chem. VL - 267 IS - 27 N2 - Spider dragline silk is a unique protein fiber possessing both high tensile strength and high elasticity. A partial cDNA clone for one dragline silk protein (Spidroin 1) was previously isolated. However, the predicted amino acid sequence could not account for the amino acid composition of dragline silk. We have isolated a partial cDNA clone for another dragline silk protein (Spidroin 2), demonstrating that dragline silk is composed of multiple proteins. The amino acid sequence exhibits an entirely different repetitive motif than Spidroin 1. Spidroin 2 is predicted to consist of linked beta-turns in proline-rich regions which alternate with beta-sheet regions composed of polyalanine segments. This structure for Spidroin 2 provides a model for dragline silk structure and function. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/1527052/Isolation_of_a_clone_encoding_a_second_dragline_silk_fibroin__Nephila_clavipes_dragline_silk_is_a_two_protein_fiber_ L2 - http://www.jbc.org/cgi/pmidlookup?view=long&pmid=1527052 DB - PRIME DP - Unbound Medicine ER -