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Nickel-responding T cells are CD4+ CLA+ CD45RO+ and express chemokine receptors CXCR3, CCR4 and CCR10.
Br J Dermatol. 2004 Jul; 151(1):32-41.BJ

Abstract

BACKGROUND

Whereas T lymphocytes are widely accepted as effector cells determining the pathogenesis of allergic contact dermatitis, contradictory results have been found regarding the roles of different T-cell subsets. The use of various experimental models, involving long-term cultured T-cell lines or clones, may explain these contradictory results.

OBJECTIVE

To investigate the involvement of distinct T-cell subsets in patients with nickel contact allergy.

METHODS

Different T-cell subsets were directly isolated from peripheral blood mononuclear cells (PBMCs) of nickel-allergic patients, and their proliferative capacity, type-1 or type-2 cytokine secretion [measured by interferon (IFN)-gamma or interleukin (IL)-5 release] and phenotypical marker expression were analysed after stimulation with nickel.

RESULTS

Only CD4+ CLA+ CD45RO+ and not CD8+ T cells proliferate and produce both type-1 (IFN-gamma) and type-2 (IL-5) cytokines in response to nickel. Moreover, cells expressing the marker CLA in combination with CD4, CD45RO or CD69 are increased after nickel-specific stimulation. Interestingly, in addition, CD45RA+ CLA+ cells showed an increased frequency after allergen-specific stimulation. Analysis of nickel-reactive T cells for expression of distinct chemokine receptors showed that both proliferative capacity and cytokine production are restricted to subsets expressing CXCR3, CCR4 but not CCR6. Fluorescence-activated cell sorting analysis of chemokine receptors expressed on nickel-stimulated T cells confirmed these results; a subset of T cells expressing CLA and CXCR3, CCR4 and, most importantly, CCR10 increased in response to allergen, while these CLA+ nickel-reactive T cells were all negative for CCR6.

CONCLUSIONS

These findings demonstrate that freshly isolated nickel-reactive T cells can be characterized as CD4+ CLA+ memory T cells which express the chemokine receptors CXCR3, CCR4 and CCR10, but not CCR6.

Authors+Show Affiliations

Department of Dermatology, VU University Medical Centre, PO Box 7057, 1007 MB Amsterdam, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15270870

Citation

Moed, H, et al. "Nickel-responding T Cells Are CD4+ CLA+ CD45RO+ and Express Chemokine Receptors CXCR3, CCR4 and CCR10." The British Journal of Dermatology, vol. 151, no. 1, 2004, pp. 32-41.
Moed H, Boorsma DM, Stoof TJ, et al. Nickel-responding T cells are CD4+ CLA+ CD45RO+ and express chemokine receptors CXCR3, CCR4 and CCR10. Br J Dermatol. 2004;151(1):32-41.
Moed, H., Boorsma, D. M., Stoof, T. J., von Blomberg, B. M., Bruynzeel, D. P., Scheper, R. J., Gibbs, S., & Rustemeyer, T. (2004). Nickel-responding T cells are CD4+ CLA+ CD45RO+ and express chemokine receptors CXCR3, CCR4 and CCR10. The British Journal of Dermatology, 151(1), 32-41.
Moed H, et al. Nickel-responding T Cells Are CD4+ CLA+ CD45RO+ and Express Chemokine Receptors CXCR3, CCR4 and CCR10. Br J Dermatol. 2004;151(1):32-41. PubMed PMID: 15270870.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nickel-responding T cells are CD4+ CLA+ CD45RO+ and express chemokine receptors CXCR3, CCR4 and CCR10. AU - Moed,H, AU - Boorsma,D M, AU - Stoof,T J, AU - von Blomberg,B M E, AU - Bruynzeel,D P, AU - Scheper,R J, AU - Gibbs,S, AU - Rustemeyer,T, PY - 2004/7/24/pubmed PY - 2004/10/20/medline PY - 2004/7/24/entrez SP - 32 EP - 41 JF - The British journal of dermatology JO - Br. J. Dermatol. VL - 151 IS - 1 N2 - BACKGROUND: Whereas T lymphocytes are widely accepted as effector cells determining the pathogenesis of allergic contact dermatitis, contradictory results have been found regarding the roles of different T-cell subsets. The use of various experimental models, involving long-term cultured T-cell lines or clones, may explain these contradictory results. OBJECTIVE: To investigate the involvement of distinct T-cell subsets in patients with nickel contact allergy. METHODS: Different T-cell subsets were directly isolated from peripheral blood mononuclear cells (PBMCs) of nickel-allergic patients, and their proliferative capacity, type-1 or type-2 cytokine secretion [measured by interferon (IFN)-gamma or interleukin (IL)-5 release] and phenotypical marker expression were analysed after stimulation with nickel. RESULTS: Only CD4+ CLA+ CD45RO+ and not CD8+ T cells proliferate and produce both type-1 (IFN-gamma) and type-2 (IL-5) cytokines in response to nickel. Moreover, cells expressing the marker CLA in combination with CD4, CD45RO or CD69 are increased after nickel-specific stimulation. Interestingly, in addition, CD45RA+ CLA+ cells showed an increased frequency after allergen-specific stimulation. Analysis of nickel-reactive T cells for expression of distinct chemokine receptors showed that both proliferative capacity and cytokine production are restricted to subsets expressing CXCR3, CCR4 but not CCR6. Fluorescence-activated cell sorting analysis of chemokine receptors expressed on nickel-stimulated T cells confirmed these results; a subset of T cells expressing CLA and CXCR3, CCR4 and, most importantly, CCR10 increased in response to allergen, while these CLA+ nickel-reactive T cells were all negative for CCR6. CONCLUSIONS: These findings demonstrate that freshly isolated nickel-reactive T cells can be characterized as CD4+ CLA+ memory T cells which express the chemokine receptors CXCR3, CCR4 and CCR10, but not CCR6. SN - 0007-0963 UR - https://www.unboundmedicine.com/medline/citation/15270870/Nickel_responding_T_cells_are_CD4+_CLA+_CD45RO+_and_express_chemokine_receptors_CXCR3_CCR4_and_CCR10_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0007-0963&date=2004&volume=151&issue=1&spage=32 DB - PRIME DP - Unbound Medicine ER -