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Expression, purification, crystallization and preliminary X-ray diffraction data of methylmalonate-semialdehyde dehydrogenase from Bacillus subtilis.
Acta Crystallogr D Biol Crystallogr. 2004 Aug; 60(Pt 8):1435-7.AC

Abstract

Methylmalonate-semialdehyde dehydrogenase from Bacillus subtilis was cloned and overexpressed in Escherichia coli. Suitable crystals for X-ray diffraction experiments were obtained by the hanging-drop vapour-diffusion method using ammonium sulfate as precipitant. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 195.2, b = 192.5, c = 83.5 A, and contain one tetramer per asymmetric unit. X-ray diffraction data were collected to 2.5 A resolution using a synchrotron-radiation source. The crystal structure was solved by the molecular-replacement method.

Authors+Show Affiliations

Dubourg H
Laboratoire de Cristallographie et Modélisation des Matériaux Minéraux et Biologiques, UMR 7036 CNRS-UHP, Groupe Biocristallographie, Faculté des Sciences et Techniques, BP 239, 54506 Vandoeuvre-lès-Nancy, France.
Stines-Chaumeil C
No affiliation info available
Didierjean C
No affiliation info available
Talfournier F
No affiliation info available
Rahuel-Clermont S
No affiliation info available
Branlant G
No affiliation info available
Aubry A
No affiliation info available

MeSH

Aldehyde OxidoreductasesBacillus subtilisCrystallizationCrystallography, X-RayGene ExpressionMethylmalonate-Semialdehyde Dehydrogenase (Acylating)

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15272169

Citation

Dubourg, Hélène, et al. "Expression, Purification, Crystallization and Preliminary X-ray Diffraction Data of Methylmalonate-semialdehyde Dehydrogenase From Bacillus Subtilis." Acta Crystallographica. Section D, Biological Crystallography, vol. 60, no. Pt 8, 2004, pp. 1435-7.
Dubourg H, Stines-Chaumeil C, Didierjean C, et al. Expression, purification, crystallization and preliminary X-ray diffraction data of methylmalonate-semialdehyde dehydrogenase from Bacillus subtilis. Acta Crystallogr D Biol Crystallogr. 2004;60(Pt 8):1435-7.
Dubourg, H., Stines-Chaumeil, C., Didierjean, C., Talfournier, F., Rahuel-Clermont, S., Branlant, G., & Aubry, A. (2004). Expression, purification, crystallization and preliminary X-ray diffraction data of methylmalonate-semialdehyde dehydrogenase from Bacillus subtilis. Acta Crystallographica. Section D, Biological Crystallography, 60(Pt 8), 1435-7.
Dubourg H, et al. Expression, Purification, Crystallization and Preliminary X-ray Diffraction Data of Methylmalonate-semialdehyde Dehydrogenase From Bacillus Subtilis. Acta Crystallogr D Biol Crystallogr. 2004;60(Pt 8):1435-7. PubMed PMID: 15272169.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression, purification, crystallization and preliminary X-ray diffraction data of methylmalonate-semialdehyde dehydrogenase from Bacillus subtilis. AU - Dubourg,Hélène, AU - Stines-Chaumeil,Claire, AU - Didierjean,Claude, AU - Talfournier,François, AU - Rahuel-Clermont,Sophie, AU - Branlant,Guy, AU - Aubry,André, Y1 - 2004/07/21/ PY - 2004/03/29/received PY - 2004/05/21/accepted PY - 2004/7/24/pubmed PY - 2005/2/19/medline PY - 2004/7/24/entrez SP - 1435 EP - 7 JF - Acta crystallographica. Section D, Biological crystallography JO - Acta Crystallogr D Biol Crystallogr VL - 60 IS - Pt 8 N2 - Methylmalonate-semialdehyde dehydrogenase from Bacillus subtilis was cloned and overexpressed in Escherichia coli. Suitable crystals for X-ray diffraction experiments were obtained by the hanging-drop vapour-diffusion method using ammonium sulfate as precipitant. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 195.2, b = 192.5, c = 83.5 A, and contain one tetramer per asymmetric unit. X-ray diffraction data were collected to 2.5 A resolution using a synchrotron-radiation source. The crystal structure was solved by the molecular-replacement method. SN - 0907-4449 UR - https://www.unboundmedicine.com/medline/citation/15272169/Expression_purification_crystallization_and_preliminary_X_ray_diffraction_data_of_methylmalonate_semialdehyde_dehydrogenase_from_Bacillus_subtilis_ DB - PRIME DP - Unbound Medicine ER -