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Expression, purification, crystallization and preliminary X-ray diffraction data of methylmalonate-semialdehyde dehydrogenase from Bacillus subtilis.
Acta Crystallogr D Biol Crystallogr. 2004 Aug; 60(Pt 8):1435-7.AC

Abstract

Methylmalonate-semialdehyde dehydrogenase from Bacillus subtilis was cloned and overexpressed in Escherichia coli. Suitable crystals for X-ray diffraction experiments were obtained by the hanging-drop vapour-diffusion method using ammonium sulfate as precipitant. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 195.2, b = 192.5, c = 83.5 A, and contain one tetramer per asymmetric unit. X-ray diffraction data were collected to 2.5 A resolution using a synchrotron-radiation source. The crystal structure was solved by the molecular-replacement method.

Authors+Show Affiliations

Laboratoire de Cristallographie et Modélisation des Matériaux Minéraux et Biologiques, UMR 7036 CNRS-UHP, Groupe Biocristallographie, Faculté des Sciences et Techniques, BP 239, 54506 Vandoeuvre-lès-Nancy, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15272169

Citation

Dubourg, Hélène, et al. "Expression, Purification, Crystallization and Preliminary X-ray Diffraction Data of Methylmalonate-semialdehyde Dehydrogenase From Bacillus Subtilis." Acta Crystallographica. Section D, Biological Crystallography, vol. 60, no. Pt 8, 2004, pp. 1435-7.
Dubourg H, Stines-Chaumeil C, Didierjean C, et al. Expression, purification, crystallization and preliminary X-ray diffraction data of methylmalonate-semialdehyde dehydrogenase from Bacillus subtilis. Acta Crystallogr D Biol Crystallogr. 2004;60(Pt 8):1435-7.
Dubourg, H., Stines-Chaumeil, C., Didierjean, C., Talfournier, F., Rahuel-Clermont, S., Branlant, G., & Aubry, A. (2004). Expression, purification, crystallization and preliminary X-ray diffraction data of methylmalonate-semialdehyde dehydrogenase from Bacillus subtilis. Acta Crystallographica. Section D, Biological Crystallography, 60(Pt 8), 1435-7.
Dubourg H, et al. Expression, Purification, Crystallization and Preliminary X-ray Diffraction Data of Methylmalonate-semialdehyde Dehydrogenase From Bacillus Subtilis. Acta Crystallogr D Biol Crystallogr. 2004;60(Pt 8):1435-7. PubMed PMID: 15272169.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression, purification, crystallization and preliminary X-ray diffraction data of methylmalonate-semialdehyde dehydrogenase from Bacillus subtilis. AU - Dubourg,Hélène, AU - Stines-Chaumeil,Claire, AU - Didierjean,Claude, AU - Talfournier,François, AU - Rahuel-Clermont,Sophie, AU - Branlant,Guy, AU - Aubry,André, Y1 - 2004/07/21/ PY - 2004/03/29/received PY - 2004/05/21/accepted PY - 2004/7/24/pubmed PY - 2005/2/19/medline PY - 2004/7/24/entrez SP - 1435 EP - 7 JF - Acta crystallographica. Section D, Biological crystallography JO - Acta Crystallogr D Biol Crystallogr VL - 60 IS - Pt 8 N2 - Methylmalonate-semialdehyde dehydrogenase from Bacillus subtilis was cloned and overexpressed in Escherichia coli. Suitable crystals for X-ray diffraction experiments were obtained by the hanging-drop vapour-diffusion method using ammonium sulfate as precipitant. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 195.2, b = 192.5, c = 83.5 A, and contain one tetramer per asymmetric unit. X-ray diffraction data were collected to 2.5 A resolution using a synchrotron-radiation source. The crystal structure was solved by the molecular-replacement method. SN - 0907-4449 UR - https://www.unboundmedicine.com/medline/citation/15272169/Expression_purification_crystallization_and_preliminary_X_ray_diffraction_data_of_methylmalonate_semialdehyde_dehydrogenase_from_Bacillus_subtilis_ DB - PRIME DP - Unbound Medicine ER -