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Integrated on-chip derivatization and electrophoresis for the rapid analysis of biogenic amines.
Electrophoresis. 2004 Jul; 25(14):2363-73.E

Abstract

We demonstrate the monolithic integration of a chemical reactor with a capillary electrophoresis device for the rapid and sensitive analysis of biogenic amines. Fluorescein isothiocyanate (FITC) is widely employed for the analysis of amino-group containing analytes. However, the slow reaction kinetics hinders the use of this dye for on-chip labeling applications. Other alternatives are available such as o-phthaldehyde (OPA), however, the inferior photophysical properties and the UV lambdamax present difficulties when using common excitation sources leading to a disparity in sensitivity. Consequently, we present for the first time the use of dichlorotriazine fluorescein (DTAF) as a superior in situ derivatizing agent for biogenic amines in microfluidic devices. The developed microdevice employs both hydrodynamic and electroosmotic flow, facilitating the creation of a polymeric microchip to perform both precolumn derivatization and electrophoretic analysis. The favorable photophysical properties of the DTAF and its fast reaction kinetics provide detection limits down to 1 nM and total analysis times (including on-chip mixing and reaction) of <60 s. The detection limits are two orders of magnitude lower than current limits obtained with both FITC and OPA. The optimized microdevice is also employed to probe biogenic amines in real samples.

Authors+Show Affiliations

Beard NP
Department of Chemistry, Imperial College London, Exhibition Road, South Kensington, London, UK.
Edel JB
No affiliation info available
deMello AJ
No affiliation info available

MeSH

Biogenic AminesElectrophoresis, CapillaryFluorescein-5-isothiocyanateFluorescent DyesMicrochip Analytical Procedures

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15274019

Citation

Beard, Nigel P., et al. "Integrated On-chip Derivatization and Electrophoresis for the Rapid Analysis of Biogenic Amines." Electrophoresis, vol. 25, no. 14, 2004, pp. 2363-73.
Beard NP, Edel JB, deMello AJ. Integrated on-chip derivatization and electrophoresis for the rapid analysis of biogenic amines. Electrophoresis. 2004;25(14):2363-73.
Beard, N. P., Edel, J. B., & deMello, A. J. (2004). Integrated on-chip derivatization and electrophoresis for the rapid analysis of biogenic amines. Electrophoresis, 25(14), 2363-73.
Beard NP, Edel JB, deMello AJ. Integrated On-chip Derivatization and Electrophoresis for the Rapid Analysis of Biogenic Amines. Electrophoresis. 2004;25(14):2363-73. PubMed PMID: 15274019.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Integrated on-chip derivatization and electrophoresis for the rapid analysis of biogenic amines. AU - Beard,Nigel P, AU - Edel,Joshua B, AU - deMello,Andrew J, PY - 2004/7/27/pubmed PY - 2005/1/27/medline PY - 2004/7/27/entrez SP - 2363 EP - 73 JF - Electrophoresis JO - Electrophoresis VL - 25 IS - 14 N2 - We demonstrate the monolithic integration of a chemical reactor with a capillary electrophoresis device for the rapid and sensitive analysis of biogenic amines. Fluorescein isothiocyanate (FITC) is widely employed for the analysis of amino-group containing analytes. However, the slow reaction kinetics hinders the use of this dye for on-chip labeling applications. Other alternatives are available such as o-phthaldehyde (OPA), however, the inferior photophysical properties and the UV lambdamax present difficulties when using common excitation sources leading to a disparity in sensitivity. Consequently, we present for the first time the use of dichlorotriazine fluorescein (DTAF) as a superior in situ derivatizing agent for biogenic amines in microfluidic devices. The developed microdevice employs both hydrodynamic and electroosmotic flow, facilitating the creation of a polymeric microchip to perform both precolumn derivatization and electrophoretic analysis. The favorable photophysical properties of the DTAF and its fast reaction kinetics provide detection limits down to 1 nM and total analysis times (including on-chip mixing and reaction) of <60 s. The detection limits are two orders of magnitude lower than current limits obtained with both FITC and OPA. The optimized microdevice is also employed to probe biogenic amines in real samples. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/15274019/Integrated_on_chip_derivatization_and_electrophoresis_for_the_rapid_analysis_of_biogenic_amines_ DB - PRIME DP - Unbound Medicine ER -