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Chromophore conformation and the evolution of tertiary structural changes in photoactive yellow protein.
Structure. 2004 Jun; 12(6):1039-45.S

Abstract

We use time-resolved crystallography to observe the structural progression of a bacterial blue light photoreceptor throughout its photocycle. Data were collected from 10 ns to 100 ms after photoactivation of the E46Q mutant of photoactive yellow protein. Refinement of transient chromophore conformations shows that the spectroscopically distinct intermediates are formed via progressive disruption of the hydrogen bond network to the chromophore. Although structural change occurs within a few nanoseconds on and around the chromophore, it takes milliseconds for a distinct pattern of tertiary structural change to fully progress through the entire molecule, thus generating the putative signaling state. Remarkably, the coupling between the chromophore conformation and the tertiary structure of this small protein is not tight: there are leads and lags between changes in the conformation of the chromophore and the protein tertiary structure.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637, USA. smander@midway.uchicago.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15274923

Citation

Anderson, Spencer, et al. "Chromophore Conformation and the Evolution of Tertiary Structural Changes in Photoactive Yellow Protein." Structure (London, England : 1993), vol. 12, no. 6, 2004, pp. 1039-45.
Anderson S, Srajer V, Pahl R, et al. Chromophore conformation and the evolution of tertiary structural changes in photoactive yellow protein. Structure. 2004;12(6):1039-45.
Anderson, S., Srajer, V., Pahl, R., Rajagopal, S., Schotte, F., Anfinrud, P., Wulff, M., & Moffat, K. (2004). Chromophore conformation and the evolution of tertiary structural changes in photoactive yellow protein. Structure (London, England : 1993), 12(6), 1039-45.
Anderson S, et al. Chromophore Conformation and the Evolution of Tertiary Structural Changes in Photoactive Yellow Protein. Structure. 2004;12(6):1039-45. PubMed PMID: 15274923.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chromophore conformation and the evolution of tertiary structural changes in photoactive yellow protein. AU - Anderson,Spencer, AU - Srajer,Vukica, AU - Pahl,Reinhard, AU - Rajagopal,Sudarshan, AU - Schotte,Friedrich, AU - Anfinrud,Philip, AU - Wulff,Michael, AU - Moffat,Keith, PY - 2004/02/26/received PY - 2004/04/01/revised PY - 2004/04/05/accepted PY - 2004/7/28/pubmed PY - 2004/8/28/medline PY - 2004/7/28/entrez SP - 1039 EP - 45 JF - Structure (London, England : 1993) JO - Structure VL - 12 IS - 6 N2 - We use time-resolved crystallography to observe the structural progression of a bacterial blue light photoreceptor throughout its photocycle. Data were collected from 10 ns to 100 ms after photoactivation of the E46Q mutant of photoactive yellow protein. Refinement of transient chromophore conformations shows that the spectroscopically distinct intermediates are formed via progressive disruption of the hydrogen bond network to the chromophore. Although structural change occurs within a few nanoseconds on and around the chromophore, it takes milliseconds for a distinct pattern of tertiary structural change to fully progress through the entire molecule, thus generating the putative signaling state. Remarkably, the coupling between the chromophore conformation and the tertiary structure of this small protein is not tight: there are leads and lags between changes in the conformation of the chromophore and the protein tertiary structure. SN - 0969-2126 UR - https://www.unboundmedicine.com/medline/citation/15274923/Chromophore_conformation_and_the_evolution_of_tertiary_structural_changes_in_photoactive_yellow_protein_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0969212604001480 DB - PRIME DP - Unbound Medicine ER -