Tags

Type your tag names separated by a space and hit enter

Differential effects of NMDA and AMPA/kainate receptor antagonists on superoxide production and MnSOD activity in rat brain following intrahippocampal injection.
Brain Res Bull. 2004 Jul 30; 64(1):85-93.BR

Abstract

The involvement of NMDA and AMPA/kainate receptors in the induction of superoxide radical production in the rat brain was examined after injection of kainate, non-NMDA receptor agonist, kainate plus 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), selective AMPA/kainate receptor antagonist, or kainate plus 2-amino-5-phosphonopentanoic acid (APV), selective NMDA receptor antagonist. Competitive glutamate receptor antagonists were injected with kainate unilaterally into the CA3 region of the rat hippocampus. We investigated superoxide production and mitochondrial MnSOD activity after injection. The measurements took place at different times (5, 15 min, 2, 48 h and 7 days) in the ipsi- and contralateral hippocampus, forebrain cortex, striatum, and cerebellum homogenates. Used glutamate antagonists APV and CNQX both expressed sufficient neuroprotection in sense of decreasing superoxide production and increasing MnSOD levels, but with differential effect in mechanisms and time dynamics. Our findings suggest that NMDA and AMPA/kainate receptors are differentially involved in superoxide production. Following intrahippocampal antagonists injection they, also, interpose different neuroprotection effect on the induction of MnSOD activity in distinct brain regions affected by the injury, which are functionally connected via afferents and efferents. It suggests that MnSOD protects the cells in these regions from superoxide-induced damage and therefore may limit the retrograde and anterograde spread of neurotoxicity.

Authors+Show Affiliations

Department of Physiology and Biochemistry, Faculty of Biology, University of Belgrade, p.f. 52, Studentski trg 16, 11000 Belgrade, Serbia and Montenegro. lira@ibiss.bg.ac.yuNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15275961

Citation

Radenovic, L, et al. "Differential Effects of NMDA and AMPA/kainate Receptor Antagonists On Superoxide Production and MnSOD Activity in Rat Brain Following Intrahippocampal Injection." Brain Research Bulletin, vol. 64, no. 1, 2004, pp. 85-93.
Radenovic L, Selakovic V, Kartelija G, et al. Differential effects of NMDA and AMPA/kainate receptor antagonists on superoxide production and MnSOD activity in rat brain following intrahippocampal injection. Brain Res Bull. 2004;64(1):85-93.
Radenovic, L., Selakovic, V., Kartelija, G., Todorovic, N., & Nedeljkovic, M. (2004). Differential effects of NMDA and AMPA/kainate receptor antagonists on superoxide production and MnSOD activity in rat brain following intrahippocampal injection. Brain Research Bulletin, 64(1), 85-93.
Radenovic L, et al. Differential Effects of NMDA and AMPA/kainate Receptor Antagonists On Superoxide Production and MnSOD Activity in Rat Brain Following Intrahippocampal Injection. Brain Res Bull. 2004 Jul 30;64(1):85-93. PubMed PMID: 15275961.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential effects of NMDA and AMPA/kainate receptor antagonists on superoxide production and MnSOD activity in rat brain following intrahippocampal injection. AU - Radenovic,L, AU - Selakovic,V, AU - Kartelija,G, AU - Todorovic,N, AU - Nedeljkovic,M, PY - 2004/04/07/received PY - 2004/05/31/revised PY - 2004/06/01/accepted PY - 2004/7/28/pubmed PY - 2004/10/16/medline PY - 2004/7/28/entrez SP - 85 EP - 93 JF - Brain research bulletin JO - Brain Res Bull VL - 64 IS - 1 N2 - The involvement of NMDA and AMPA/kainate receptors in the induction of superoxide radical production in the rat brain was examined after injection of kainate, non-NMDA receptor agonist, kainate plus 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), selective AMPA/kainate receptor antagonist, or kainate plus 2-amino-5-phosphonopentanoic acid (APV), selective NMDA receptor antagonist. Competitive glutamate receptor antagonists were injected with kainate unilaterally into the CA3 region of the rat hippocampus. We investigated superoxide production and mitochondrial MnSOD activity after injection. The measurements took place at different times (5, 15 min, 2, 48 h and 7 days) in the ipsi- and contralateral hippocampus, forebrain cortex, striatum, and cerebellum homogenates. Used glutamate antagonists APV and CNQX both expressed sufficient neuroprotection in sense of decreasing superoxide production and increasing MnSOD levels, but with differential effect in mechanisms and time dynamics. Our findings suggest that NMDA and AMPA/kainate receptors are differentially involved in superoxide production. Following intrahippocampal antagonists injection they, also, interpose different neuroprotection effect on the induction of MnSOD activity in distinct brain regions affected by the injury, which are functionally connected via afferents and efferents. It suggests that MnSOD protects the cells in these regions from superoxide-induced damage and therefore may limit the retrograde and anterograde spread of neurotoxicity. SN - 0361-9230 UR - https://www.unboundmedicine.com/medline/citation/15275961/Differential_effects_of_NMDA_and_AMPA/kainate_receptor_antagonists_on_superoxide_production_and_MnSOD_activity_in_rat_brain_following_intrahippocampal_injection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0361923004001388 DB - PRIME DP - Unbound Medicine ER -